of farUV: 180-240nm. 1. Near UV CD: 240n-320nm‚ Aromatic amino acids and disulphide bonds. 2. Visible CD: d-d transition in some metal protein complexes for eg Cu (II) prion. Principles of Chromatography Substances present in a mixture are allowed to distribute themselves between two phases: the stationary phase (fixed) and the mobile phase. As the mobile phase flows over the stationary phase‚ components of the mixture experience many transfers
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Chromatography is a process used to separate mixtures. The word chromatography is derived from the Greek words ‘khroma’ and ‘graphein’ meaning ‘color’ and ‘to write’ or to represent. Although there are a couple different types of chromatography‚ in each case a substance is placed onto or into a medium and a solvent is passed through the test substance. In chromatography science‚ the solvent is called the mobile phase or the carrier fluid and the medium is called the stationary phase. There are four
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Gas Chromatography Purpose: The purpose of the gas chromatography lab is to find out how different substances interact with the surface of a solid. Chromatography is a separation technique that depends on the relative distribution of the components of a mixture between a mobile phase and a solid stationary phase. Chromatography measures the tendency of a substance to interact with the surface of a solid or to remain in a mobile phase. When doing a chromatography lab the mobile phase has to
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Paper Chromatography Chromatography comes from the Greek word khromatos (color) and graphein (to write); i.e. paper chromatography is literally color writing on paper. This method tests the purity of compounds and identifies substances. This analytical process‚ despite having been replaced by the success of thin layer chromatography‚ still stands as a valuable teaching tool‚ and is nevertheless very common. This method is very useful because not only is it a relatively quick process‚ but also
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Distillation Column In the the Chemical Enginneering Lab at CSULB there is a total reflux eight-stage glass binary distillation column at atmospheric pressure. The components that need to be separated are 1-propanol and 2-propanol. These two compounds are isomers with fairly close boiling points. They are separated based on their physical propertis. With the
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complex themes and often tradition-breaking attributes‚ making them key components of the feminist movement‚ and The Broken Column is no exception. Frida Kahlo’s The Broken Column expresses her self-reflection on her own body and past experiences as well as challenging classically held ideas about the male gaze and the role of female artists in the sublime. The Broken Column‚ an oil on Masonite painting at 39.8 by 30.6 centimeters‚ provides a detailed self-reflection on the impact of her injuries
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Financial Analysis: Dr. Pepper Snapple Group vs. Coca-Cola Analyzing and comparing the financial statements of Coca-Cola (KO) and Dr. Pepper Snapple Group (DPS) for the year 2010 will expose the strengths and weaknesses of Dr. Pepper Snapple group compared to Coca-Cola. Liquidity ratios are used to determine a business’s ability to pay off its short-term debt obligations. The first liquidity ratio I used in my analysis is the current ratio. Coca-Cola has a current ratio of 1.17 and DPS has a
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DR. Pepper Current marketing factor Brand History Dr Pepper was created at Morrison¡¯s Old Corner Drug store in Waco Texas in 1885‚ making it the oldest soft drink in the United States. It was first created by Charles Alderton‚ a pharmacist‚ when he mixed several fruit flavored carbonated beverages. After creating a flavor he liked his boss test-tasted it and decided to serve it at their soda fountain. Popularity grew and soon other soda fountain operators wanted to sell it so Morrison began
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Community Outreach & Education Program CHROMATOGRAPHY (Adapted from: Forensic Science Activities. University of Colorado Boulder Hughes Initiative. .) DESCRIPTION: Students will use paper chromatography to separate ink molecules and identify the pen used on an unknown sample of handwriting. Students will graph and analyze data they collect using paper chromatography. PURPOSE/GOAL: Students will be able to: • Gain understanding of the purpose of chromatography. • Measure and graph pigment separation
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Introduction Paper chromatography is a method using a chromatography paper to identify the many mixtures of one simple color. The word chromatography comes from the Greek words‚ "chroma‚" meaning color‚ and "grafein‚" meaning to write. The process of this experiment is to place a colored dot‚ such as marker ink‚ onto the chromatography paper‚ one centimeter away from the triangular tip. Next dip your chromatography paper into the solvent‚ not allowing it to touch the colored dot. Let it sit for
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