Organic Chem II Lab 2/15/15 Friedel-Crafts Acylation of Ferrocene and Column Chromatography Introduction: The intention of this lab is to analyze the formation of acetylferrocene using column chromatography. The Friedel-Crafts acylation reaction created acetylferrocene and diacetylferrocene‚ using phosphoric acid as a catalyst for the reaction between acetic anhydride and ferrocene (once applying heat). During column chromatography‚ a solution is passed through a filtration system of silica‚ sand‚
Premium Solvent Acetic anhydride Chemistry
Column and Thin Layer Chromatography: The Separation of Spinach Pigment Abstract Spinach extract was separated into fractions containing compounds of similar polarity by column chromatography. Based on solid-liquid phase partitioning‚ this separation technique exploited the different polarity of the compounds in the spinach extract. Three fractions with different colors were obtained. The extract and its fractions were analyzed using thin layer chromatography (TLC). The TLC results showed that
Free Chromatography Thin layer chromatography
Maksin Organic Chemistry Laboratory 1 Column and Thin Layer Chromatography: The Separation of Spinach Pigments Russ Hoburg 02/15/2012 Abstract The main goal in doing the column and thin layer chromatography was to separate spinach extract into its components based on polarity and then to analyze the components. The separation of the spinach extract was done using the column chromatography with the wet/slurry packing method. Alumina was inserted into the column to act as the stationary phase. After
Premium Psychology Family Management
Column and Thin layer Chromatography of Red Siling Labuyo Marian Angelu Ramos‚ Rose Ann Refuela‚ Leomarie Duanne Sanchez‚ Paula Lynne Santos‚ Geraline Sarmiento‚ Jon Carlo Semana Group number Seven‚ 2E- Pharmacy‚ Faculty of Pharmacy‚ University of Santo Tomas ABSTRACT Chromatography is the separation of mixtures into their constituents. It relies on the differential solubilities or absorptivities of the components to be separated with respect to two phases‚ one of which is stationary and the
Free Chromatography Thin layer chromatography
COLUMN AND THIN LAYER CHROMATOGRAPHY Maria Janine B. Abarientos‚ Kuia B. Allarde‚ Aliana Keshia P. Andino Mary Viadelle E. Andrada and Nina Marian Robelea G. Ang Group 1 2C Pharmacy Organic Chemistry Laboratory ABSTRACT In this experiment‚ the techniques column and thin layer chromatography was used to separate and determine the purity of the colored components of siling labuyo and malunggay leaves. The results obtained relied on differential solubilities and adsorptivities of the components to
Premium Chromatography Thin layer chromatography
Chromatography (Greek for ‘colour writing’) is used to describe various methods applied to separate mixtures (referred to as the sample of the experiment) with great accuracy to analyze them. By using chromatography we can manipulate these to move at different speeds through the system‚ thus separating them. Chromatography is necessary in chemical industries‚ as well as bio processing companies. Chromatography can be: 1. analytical: used to measure ratios of analytes(substance in simpler forms)
Premium Chromatography High performance liquid chromatography
Background Information and Research Paper chromatography uses selective adsorption on a strip of paper. It is used for separating and identifying mixtures that are or can be colored‚ especially pigments. This can also be used in secondary or primary colors in ink experiments. Is it used for Contaminants in rainwater‚ Analysis of narcotics‚ and Detection of substances in urine. Purpose • The purpose of this lab is to see the separation of dye on the candy. Data and Observations Marker Color
Premium Color Pigment Purple
Liquid Chromatography Purpose: The purpose of this lab was to separate substances based on their polarity by using liquid chromatography. Data Table: Red Dye Blue Dye Run#1 Run#2 Run#3 Run#1 Run#2 Run#3 Start of Band(mL) 1.50 2.20 1.00 2.70 3.00 2.00 End of Band(mL) 2.70 3.00 2.00 6.40 5.50 6.00 Beaker Eluant Observations 1 H2O White powder 2 5%isopropyl Red powder 3 28%isopropyl Blue powder 4 70%isopropyl Oily residual Calculations: W = Vend – Vstart
Premium Chemical polarity Atom Oxygen
The Red Hot Chili Peppers Nick Gregory General Purpose: To Inform Specific Purpose: To Inform the audience of the history and achievements of the Red Hot Chili Peppers Central Idea: There is more to The Red Hot Chili Peppers than just a few hit songs INTRODUCTION I. Attention Getter: The Red hot Chili Peppers are more than just your average band. Currently they have won six Grammy awards and sold over 55 million albums worldwide. II. Preview: Tonight I am going to explain briefly the history
Premium
The goal of this lab was to create and purify DHFR from BL21 ecoli using column chromatography. To know if we isolate DHFR their will be one band at 53 KD or above 50 KD and in our desalted eluate page should have the highestOD₂₈₀ which translates to the highest enzyme activity .Column chromatography is a method used to purify individual chemical compounds from mixtures of compounds. The main advantage of column chromatography is the relatively low-cost and disposability of the stationary phase used
Premium Chromatography High performance liquid chromatography Chemistry