Compilation of “Dehydration of Methylcyclohexanol” Blog entries: A common Sophomore Organic Chemistry laboratory experiment that has great potential for further research is the acid catalyzed dehydration of simple alcohols. The classic dehydration of 2-methylcyclohexanol experiment that was introduced in Journal of Chemical Education in 1967 Taber(1967)JCE:44‚p620. The rather simple procedure of distilling an alcohol with an aqueous acid has spawned several investigations that have resulted
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Aliivibrio fischeri (A. fischeri) lux operon from the re-isolated plasmids after transforming the E. coli with the plasmid library. There are multiple steps for this experiment starting with the isolation of A. fischeri chDNA. Using Qiagen DNeasy Mini Spin Column Kit‚ three different samples of 100 uL of isolated A. fischeri chDNA solution each were obtained. Next‚ to determine the purity and concentration of the isolated A. fischeri chDNA samples‚ UV spectrophotometry was performed using two different spectrophotometers
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CHM130 Lab 9 Chromatography Name: Karlee Rose A. Data Table (12 points) Paper # Color Source Solvent Distance Component Moves Distance Solvent Moves Rf value 1 Yellow M&M Candies 0.1% Salt Solution 28.88mm 42mm 0.69 2 Yellow Reese’s Pieces 0.1% Salt Solution 16.95mm 32mm 0.53 3 Purple Grape Soda 0.1% Salt Solution 32.15mm 51mm 0.63 4 Purple Grape Koolaid 0.1% Salt Solution 12.12mm 31mm 0.39 5 Red Easter Egg Dye 0.1% Salt Solution 1.18mm 7mm 0.17 6 Red Dry Erase Marker
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powdered and preserved in a refrigerator at -80°C. Five hundred gm from the powdered seeds were defatted with hexane‚ then extracted with methanol (MeOH - 70%) four times‚ then concentrated under vacuum to yield 37 g. Polyamide and sephadex LH-20 chromatography columns were used to fraction the MeOH (70%) extract of Cajanus cajan. Seven flavonoid fractions were collected and identified as follows: apigenin‚ isorhamnetin‚ luteolin‚ orientin‚ quercetin‚ quercetin-3-O-B-D-glucopyranoside‚ vitexin. The pharmacological
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Another way to improve this procedure would be to add a second 15 mL portion of NaCl to the separatory funnel to make sure the aqueous layer was fully removed. Additionally‚ column chromatography could have been used in this procedure in order to purify the product. To further improve this procedure; a color change titration technique could have been used in order to quickly determine if the product was formed before running an
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|Thesis Summary | | | | | |
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Experiment 9 Thin Layer Chromatography Experiment: 9 Title: Thin Layer Chromatography Introduction: Chemical analysis is conducted on specific analytes (specific substance of interest in a mixture)‚ however it is often found that these analytes must be separated for the chemical analysis to conduct their analysis. Chromatography is a set up of laboratory a technique that is used to separate a chemical mixture. This technique is very useful as it allows us to follow the course of
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heating it. • Always wear department approved eye goggles at all times. • Long pants and closed toe shoes are required. Table of Reagents Reagent Mol. Wt. (g/mol) Bp ( ͦ C) Mp ( ͦ C) Density (g/ml) Acetanilide 135.17 304 113-115 1.219 Fluorene
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Lab Report: Understanding Photosynthesis Gen Biology Lab Abstract: This lab was called photosynthesis: understanding photosynthesis. It is a highly complex process that needs to be broken down in many steps to understand how it works. This lab covers the big components in photosynthesis including carbon dioxide intake‚ light consumption‚ and varying pigmentation. Introduction: Photosynthesis is a huge concept to learn and understand in the field of biology. Plants have their own special
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BIOLOGY 1010L – BOTANY Laboratory Module 8. Plant Physiology (2): Plant Pigment Paper Chromatography All organisms need energy for their metabolic processes. They also need “food” to produce that energy. Plants are autotrophs (self-feeders). Plants produce their food through a process called Photosynthesis. The food that they produce is the sugar glucose. Animals and other organisms are heterotrophs (other- feeders). They must consume other organisms (plants) in order to eventually get their
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