CHM 510 LABORATORY REPORT Experiment 1: Gas Chromatography (GC): Optimization of Flow Rate and Column Temperature Name: AFIQ B. ANWAR Student No.: 2012621072 (AS2253A) Date of experiment: Date of report submission: Lecturer’s Name: PN. HALIZA Gas Chromatography (GC): Optimization of Flow Rate and Column Temperature INTRODUCTION The main purpose of the experiment is to investigate the effects of column temperature and flow rate on the separation of methyl esters compounds
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Other Important Topics in Column Design Outline Sub-Flowsheets Thermodynamic model (Fluid Package) selection Solver selection Sub-flowsheets HYSYS has a multi-flowsheet architecture. A big flowsheet can be split up into smaller sub-flowsheets. Each sub-flowsheet has its own streams‚ operations‚ PFD and an independent fluid package. The column in HYSYS is a sub-flowsheet where independent operations are possible H83 PS1/H84 CFL Templates A template
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exchange Chromatography Ion exchange chromatography is a process for separating proteins and other molecules in a solution based on differences in net charge. Ion Exchange Chromatography relies on charge-charge interactions between the proteins in your sample and the charges immobilized on the resin of your choice. Ion exchange chromatography can be subdivided into cation exchange chromatography‚ in which positively charged ions bind to a negatively charged resin; and anion exchange chromatography‚ in
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favorite candies? Chromatography can solve that for you. Chromatography is a separation technique used by scientists for separating both organic and inorganic compounds. There are four different types of chromatography: thin layer‚ liquid‚ gas‚ and paper‚ but for this lab paper chromatography will be used. Who invented chromatography? A Russian botanist named Mikhail Semyonovich Tsvet invented chromatography in 1901 while doing research on plant pigments. Why is chromatography so important? This
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SINGAPORE Chemical Engineering Process Laboratory II Experiment B2 Chromatography for Protein Purification Name Matric No. Group : : : Date of Expt. : GRADE : A. Learning objectives 1. 2. 3. 4. Establish chromatographic assay to determine protein concentrations in a mixture. Appreciate the importance of resolution in protein chromatography. Understand the tension between purity and yield in protein chromatography. Understand the importance of mass balance closure in protein purification
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Gas chromatography (GC) is a chromatography technique where the separation of individual components (analytes) from a sample relies on their differing distribution between a mobile and stationary phase. The mobile phase carries the analytes through the stationary phase. In GC‚ it’s an inert gas (usually helium or nitrogen). The gas must be inert‚ so it won’t react with the sample to give a false reading. The stationary phase is a substance fixed in place to which the sample adsorbs because
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Plant Pigment Chromatography VII. Analysis (Questions) 1. What factors are involved in the separation of pigments? Factors that are involved in the separation of pigments can include pigment solubility‚ the attraction between the pigments and paper and the size of each pigment particle. Because of these factors the results were as they were. Beta-carotene traveled the furthest because it forms no hydrogen bonds to the chromatography paper and is slightly soluble in the solvent. Contrastingly
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Data and Conclusions: The purpose of this experiment was to learn how to use distillation and gas chromatography to separate and identify different compounds from a given mixture. There are several kinds of distillation methods. However‚ the method that we used in this experiment was fractional distillation. This method is used when trying to separate two different volatile compounds whose boiling points differ by 40-50°C or more. If the boiling points are too close‚ this method
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Chromatography • • • Separation based on polarity of compounds Two potential phases for a compound to exist in: mobile and stationary Partitioning of compounds between mobile phase and stationary phase occurs: o Compounds that are less polar move more in the mobile phase‚ those that are more polar “stick” more on the stationary phase o These polarity differences cause compounds move at different rates and therefore can be separated 1. Mobile Phase: the phase the moves; can be gas or
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Review Bubble column reactors Nigar Kantarcia‚ Fahir Borakb‚ Kutlu O. Ulgena‚* aDepartment of Chemical Engineering‚ Bog˘azic¸i University‚ 34342 Bebek-Istanbul‚ Turkey bDepartment of Chemical Engineering‚ Yeditepe University‚ 34755 Kadikoy-Istanbul‚ Turkey Received 31 August 2004; accepted 26 October 2004 Abstract Bubble columns are intensively used as multiphase contactors and reactors in chemical‚ biochemical and petrochemical industries. They provide several advantages during operation
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