CHEM 3152-003 Experiment 2: Fractional Distillation and Gas Chromatography Introduction: The purpose of this experiment was to separate a mixture of ethyl acetate (EtOAc) and toluene by using the method of fractional distillation and to analyze the fractions using gas chromatography (GC). ethyl acetate toluene Distillation is a method known as separating or purifying a liquid or mixture by vaporization and condensation. In a simple distillation‚ the liquids being separated boil below 150 0C
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It was concluded that the product created was paracetamol when a thin layer chromatography test was performed and observed under ultraviolet light (which is discussed further down the page). An unknown substance was treated with acetic anhydrate and resulted with paracetamol. A compound that behaves in this matter is 4-aminophenol and is widespread in the industrial production of this drug. Upon these observations was the build up of “Chemical A” decided. When 4-aminophenol is t treated with
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Standing over fifty-eight feet high is the Marble Column from the Temple of Artemis at Sardis‚ known as one of the seven wonders of the world. The marble column that stands tall at the Metropolitan Museum of Art is different from the rest found at the site of the temple because the capital is slightly smaller than the rest. This indicates that it did not belong to the outer colonnade. The columns at the Temple of Artemis are found to be ionic orders‚ being one of the three different types of orders
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distillation and gas chromatography based on their difference in boiling points. The boiling point of hexane is 69 degrees Celsius and the boiling point of toluene is 110 degrees Celsius. Three fractions were collected for both simple and fractional distillation. The first fraction was hexane since it had the lower boiling point. The second fraction was a mixture of toluene and hexane. The third fraction was toluene since it had the high boiling point of 110 degrees Celsius. Gas chromatography was also preformed
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he backbone (vertebral column) is the central axis of the skeleton. It extends from the base of the skull to the bottom of the pelvis. It consists of 26 individual bones grouped into five separate regions; The vertebrae‚ twelve thoracic vertebrae‚ five lumbar vertebrae‚ one sacral bone and one coccygeal bone. (Cinnamon L. VanPutte‚ Jennifer L. Regan‚ Andrew F. Russo‚ McGraw-Hill‚ 2010.) The Vertebral column also host major curvatures; The sacral and thoracic both concaving anteriorly and the lumbar
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foreground the composition‚ wearing a flowing white skirt and a corset of white straps that wrap around her shoulders and upper body. Kahlo’s breasts are exposed and the front of her upper body is bisected‚ revealing that “her spinal column is replaced by a broken Doric column.” In addition‚ several nails protrude from the skin on her face‚ arms‚ torso‚ and legs. In the background‚ a blue sky with white clouds hangs over the cracked‚ fissured landscape where Frida stands. Unlike The Two Fridas‚ this
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This lab is an opportunity to observe‚ study‚ and learn about ecosystems and how the environment sustains life but in a much simpler and smaller scale which is called Eco Columns. The environmental conditions in the Eco Column could sustain life for several weeks‚ therefore over the course of the several weeks we will observe and study the interconnections between each ecosystems. A self sustaining requires components such as energy flow and chemical cycle. This experiment will evaluate our knowledge
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Gas Chromatography: Purifying alcohol Introduction The goal of this lab is to understand the principles of chromatography by purifying alcohol using fractional distillation. Running standards with gas chromatography we were able to see and calibrate our data to find not only how much ethanol our alcohol attained but also what a mixed unknown sample contained. Chromatography is a way of being able to separate substances in solution that can help not only identify the analytes (the studied
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ANALYSIS OF MOUTHWASH Background Information: Commercial mouthwashes are mixtures of water‚ alcohol‚ dyes‚ flavorings‚ and other compounds. In this experiment‚ you will use gas chromatography to determine the alcohol content of the mouthwash. Since the volumes of alcohol and water are not additive when mixed‚ a calibration curve must be used. It will provide the correction factor needed for alcohol-water mixtures. The calibration curve will be prepared by placing a known amount of alcohol
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Fluorescent Protein (rGFP) From E. coli strain‚ BL21(DE3)‚ Using Ni2+-Agarose Affinity Chromatography Abstract: The purpose of these series of experiments was to express and purify recombinant Green Fluorescent Protein (rGFP) from the E. coli strain‚ BL21(DE3) by beginning with its purification via a Ni2+-agarose affinity chromatography column. The His6 tag of the rGFP bound to the Ni2+-agarose column and washes and elutions were obtained‚ with elution 3 containing the most amount of fluorescence
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