al.Qaimare Rifqa Shawer Lab report 5 21-3-2013 Adsorption Chromatography Introduction: The technique of adsorption chromatography may be illustrated by a description of how two dyes are separated when a solution of the dyes is passed through a column of powdered silica contained in a vertical glass tube‚ it’s a method used to separate solids or liquids. At the first the two dyes are adsorbed at the top of the column‚ giving this region a nearly dark blue color. However‚ when the flow
Premium Chromatography Separation process Green
NOV 09 How product giving is a ‘slam dunk’ for retailers By ELLEN DAVIS‚ VP AND NRF SPOKESPERSON | Published: NOVEMBER 9‚ 2011 Be the first to comment | This entry was posted in Holidays‚ Loss Prevention‚ Retail Companies‚ Sustainability Donating unwanted products to organizations that help people in times of need is undoubtedly a win-win for retailers. But as many companies have found‚ getting the right products to the right people isn’t always easy. Recipients have to be vetted‚ distribution
Premium Donation
Introduction In order to understand what HPLC‚ that is‚ High Performance Chromatography‚ it is necessary to understand the meaning of the term chromatography itself. Chromatography Chromatography is defined as a procedure by which inert materials and drug principles encountered in pharmaceuticals preparations are separated by fractional extraction‚ adsorption‚ or ion exchange on a porous solid. Chromatography is particularly useful as a means of separating and purifying complex and closely
Premium Chromatography High performance liquid chromatography
Analysis of Ethanol in Moonshine | Using the GC-MS | Jennifer Greene | ------------------------------------------------- 4 May 2012 ------------------------------------------------- 4 May 2012 Introduction: In this experiment gas chromatography is applied to separate the water-ethanol mixture. This method is often used in the determination of alcohol in blood or urine. One obvious application is when law enforcement agencies need to determine whether or not someone is inebriated. In
Premium Gas chromatography Chromatography Ethanol
Experiment 5 Analysis of Plant Pigment Purpose: Chromatography is used for separation. In this case the separation of plant pigments. After placing the plant sample in the column the separation will be easy to see and the samples will be analyzed using VIS spectrophotometry. This is used to isolate and identify the different lipids causing the different pigmentations. Introduction: Lipids are chemically diverse substances found in cells. Lipids are water-insoluble‚ structural components
Premium Chromatography High performance liquid chromatography
Column Chromatography ________________________________________________ You have already performed two chromatography experiments: gas chromatography and thin layer chromatography. All chromatography experiments involve passing a mixture of analytes through a system that includes a mobile phase and a stationary phase. The partitioning of the analytes between these two phases determines the rate at which they pass through the system‚ and (in theory) allows them to be separated from one another. Column
Premium Thin layer chromatography Chromatography Silica gel
from Tomato Paste Goal / Purpose To extract the carotenoid pigments from canned tomato paste and separate them by column chromatography to obtain a solution that contains lycopene. Then record the ultraviolet-visible spectrum of this solution and analyze it for evidence of isomerization. Calculation List of Materials 4.0g tomato paste screw cap vial Chromatography column 25ml buret 50ml flask ring stamd and clamp 50% mixture of acetone and low boiling petroleum ether 5ml extraction
Premium Lycopene Chromatography Solvent
is due to the structure of disperse red 9 being more symmetrical than that of disperse blue 3 and having more nonpolar bonds. Disperse blue 3 is more polar because it has a hydroxide bond and has a larger dipole. The principle behind using column chromatography is that it separates compounds based on polarity. The alumina serves to allow for a purer separation than TLC plates because it has a more polar surface than silica gel does. The less polar dye moves first because it is not as soluble in the
Premium Chromatography Thin layer chromatography Separation process
protein capture by Protein A affinity chromatography helps to remove a large majority of the impurities whicn include viruses and media components. At Stage 3‚ prior to cation exchange chromatography‚ the purified material in the eluted stream is then subjected to freezing‚ thawing and pooling. Stage 4 involves the Solvent/Detergent (S/D) viral inactivation which is the first dedicated viral clearance step. At Stage 5‚ the product undergoes cation exchange chromatography which aids in removing the S/D reagents
Premium DNA Molecular biology Protein
Title of experiment 3: Gel Filtration Chromatography of LDH INTRODUCTION Gel filtration chromatography is a type of column chromatography in which separated protein‚ peptides and amino acids on their molecular size. The stationary phase consists of beads containing pores. The mobile phase is the solvent that is found both around the beads and in the pores of the stationary phase matrix. As the sample is passes through the column‚ the molecule that are larger than the pores will not retarded by
Premium Chromatography Protein High performance liquid chromatography