Determining the Unknown Microorganism from a Mixed Culture Mylah Ferland‚ Rivier University MICRO 206‚ March 16th‚ 2016 Introduction: An unknown bacterium experiment‚ this experiment was done to discover what bacterium was in a mixed culture sample. This experiment worked with enteric bacteria which are members of the Enterobacteriaceae and live in the intestinal tract (Willey et al.‚ 2014). Also found in Willey et al (2014) is some characteristics of enterics such as they all degrade sugars by
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charcoal agar * Borrelia burgdorferi SEM * Campylobacter jejuni colonies and microscopy * Campylobacter jejuni SEM * Capnocytophaga sp. * Chryseobacterium indologenes on Mueller-Hinton * Chryseobacterium indologenes colonies * Citrobacter freundii colonies on MacConkey * Escherichia coli on blood agar | * Escherichia coli on MacConkey *
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Lahela Correa 12/08/2009 Microbiology 140 Matthew Tuthill Unknown Lab Report Introduction There are many reasons for knowing the identity of microorganisms. The reasons range from knowing the causative agent of a disease in a patient‚ so as to know how it can be treated‚ to knowing the correct microorganism to be used for making certain foods or antibiotics. This study was done by applying all of the methods that I have been learned so far in the microbiology laboratory class for the identification
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Abstract Because water serves as vehicles in the transmission of many pathogens‚ coliforms are commonly used as indicators to monitor the quality of water. I hypothesized that Lake Thoreau would have more fecal coliforms because it is a more stagnant environment meaning more animals can stop their and release their feces‚ and also the animals that reside there continue to reproduce‚ leaving no exit for their excretions. By using the MPN test‚ I was able to detect the coliforms from the fermentation
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Citrate Test: Some bacteria can use citrate as a source of carbon. To test if the unknown bacteria uses citrate as a source of carbon‚ Simmon’s citrate agar was used as the medium on which the bacteria was grown. The Simmon’s citrate agar consists of sodium citrate as the source of carbon‚ ammonium dihydrogen phosphate as the source of nitrogen along with pH indicator such as bromothymol blue. Procedure: The Citratase activity was detected by inoculating the unknown bacteria on the slant surface
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Identification of Proteus vulgaris from an Unknown Sample Lakhram Bhisham March 31‚ 2016 01:447:390 General Microbiology TA: Jennifer Goff ABSTRACT This report delineates how unknown #405 was identified as Proteus vulgaris out of a possible seven species of Enterobacteriaceae by applying various tests that are able to distinguish between members of the family. Inevitably‚ the results for many tests are identical across multiple species‚ which is expected due to the organisms’ evolutionary relatedness
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Introduction Although bacteria is microscopic in size‚ it is largely important in the healthcare field‚ environmental work‚ food preparation‚ as well as many other industries. In particular‚ it is essential that healthcare workers be able to identify the species of bacteria invading a human reservoir in order to prescribe the correct antibiotic that will kill that species. For the purpose of bacteria identification‚ numerous tests have been devised to find out the exact species in question. However
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Pure Culture Techniques In this first lab‚ you will be learning some very fundamental and important techniques. As is the case with most things‚ shorts cuts usually get you in trouble. This is especially true in Microbiology. The techniques you will be learning tonight‚ if mastered correctly‚ will make your life and learning experience in Microbiology much easier‚ if you don’t pay attention and practice these techniques incorrectly‚ well then……? Today you will be learning the following techniques:
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The Results from the Gram positive tests indicated that the unknown #4 was Streptococcus pyogenes. All seven tests on the unknown matched S. pyogenes perfectly. The blood agar plate proved the unknown to be β hemolytic‚ meaning the unknown bacteria was capable of complete hemolysis. This test separated the unknown into the β Streptococcus group‚ narrowing the possible bacteria to S. aureus or S. pyogenes. The Catalase test was used to determine if the unknown could break down hydrogen peroxide
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Introduction To date‚ the bacterial resistance to antibiotics is one of the most important global health threat introduced by the World Health Organization (WHO) and most annual mortality due to hospital infections occurs because of this challenge (1). According to the US Centers for Disease Control and Prevention (CDC) report‚ carbapenem-resistant Enterobacteriaceae (CRE) are one of the three main antibiotic resistance threats. (2). Enterobacteriaceae are Gram-negative bacilli which cause a wide
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