the input of two ATPs to produce two pyruvates‚ two NADHs‚ and 4 ATPs. The NADHs are synthesised when NAD+‚ delivered by B vitamins‚ become bound to hydrogen and energised electrons1. Following glycolysis is the Krebs cycle and electron transport chain respectively. The Krebs cycle uses the two pyruvates produced in glycolysis to make 2 ATPs per glucose and energy. This occurs through the oxidation of a pyruvate‚ wherein one of the three carbons will bond to oxygen and leave the cell as carbon dioxide
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water potential solutions and detergents) to analyse where various enzymes in the mitochondria work as well as the fact that ETC enzymes are imbedded in the inner mitochondrial membrane‚ away from the matrix (where the link reaction and the Krebs cycle take place). Oxidative phosphorylation did not take place without the outer membrane
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The Building Blocks of Life Worksheet Part 1: Mitosis and Meiosis Short-Answer Response Why are the process of mitosis and meiosis both important to a living organism? The process of mitosis and meiosis are both important to a living organism because without them a cell would not be able to reproduce. Mitosis is an asexual process used to replace old and dead cells with new ones by dividing into two identical daughter cells. Meiosis‚ however‚ is the step that makes sexual reproduction possible
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Pyruvate (c3) Carbon is lost here d/t expire- Acetyl group (c2) then goes to Kreb cycle in the mitochondria. Glucose(c6) – Pyruvate (c3)- Acetyl group + COA = Acetyl CoA –(c2) (looses Co2 ( (joined with a chemical bond) As soon as it enters the Matrix) – Goes into the Kreb cycle then into ETC. DECARBOXYLATION = Removing of C02 from Pyruvate to produce
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Protocol for Lab 5 – Aerobic Respiration Part 1 Isolation of Mitochondria from Cauliflower - Weigh 50g of rosettes cut from fresh cauliflower head. - Cut rosettes and place it on ice - Prepare juice extractor by placing ice and an empty 150 ml beaker into the right compartment. - Collect pulp from left compartment and record total volume of the extract. Approx. 20ml - Filter the pulp using six layered cheese cloth and collect it in a beaker sitting on ice. - Place two 50 ml test tubes
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Introduction: Cytochrome c oxidase plays a vital role in cellular respiration by accepting e- from cytochrome c and transferring them to an acceptor oxygen molecule in the final step of electron transfer chain. Carbon monoxide and cyanide are few of the inhibitor of this enzyme. 4 Fe2+ -cyt c + 8H+ + O2 4 Fe3+ -cyt c + 2H2O + 4H+ [out] Cytochrome c oxidase locates to the inner membrane which separates the mitochondrial matrix from the intermembrane space. However‚ Potato tubes can
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The first step in the steroidogenesis takes place within the mitochondria where CHOL becomes a substrate . The transport of free CHOL from the outer mitochondrial membrane to the inner mitochondrial membrane is facilitated by the steroidogenic acute regulatory protein (StAR). Within the mitochondria CHOL is converted to pregnenolone (PREG) by the side-chain cleavage enzyme known as CYP11A1. The conversion to PREG is the rate-limiting step and the following intermediate steps are taken place in the
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12. Briefly discuss the role of the electron transport chain in cellular respiration. In your explanation‚ discuss and identify key molecules involved in this process and explain their relationship which each other in terms of how they contribute to the main cellular purpose of this process. Electron transport chain plays a key role of in the production of oxygen in cellular respiration. ETC uses many enzymes located on the inner mitochondrial membrane during the production of oxygen. The source
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Every physical action we take requires some sort of energy that our body produces. The most important energy molecule in the cell that our body uses is adenosine triphosphate (ATP). ATP is “a high-energy molecule that stores a considerable amount of energy within the chemical bonds that hold the molecule together” (Exercise Physiology: Tennis Physiology). ATP results from energy being produced from either an aerobic system or anaerobic system. The production of ATP can come from systems such as ATP-PC
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Abstract: In this Lab we used the chemical DPIP to detect the rate of succinate broken down by the mitochondrial solution. We detected the amount of DPIP in the solution with a spectrophotometer and measuring the absorbance of light at the 600nm range. DPIP is a useful chemical to use in this experiment because it goes from a blue color when oxidized to a colorless liquid (Ogura‚ 281)‚ this is due to the hydrogen ions and electrons released during the transitional step between succinate and fumarate
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