of farUV: 180-240nm. 1. Near UV CD: 240n-320nm‚ Aromatic amino acids and disulphide bonds. 2. Visible CD: d-d transition in some metal protein complexes for eg Cu (II) prion. Principles of Chromatography Substances present in a mixture are allowed to distribute themselves between two phases: the stationary phase (fixed) and the mobile phase. As the mobile phase flows over the stationary phase‚ components of the mixture experience many transfers
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pigments using chromatography‚ calculate Rf values using the collected data‚ and study photosynthesis with isolated chloroplasts. Light energy Light energy Background Information (Activity A): In photosynthesis‚ plant cells convert light energy into chemical energy that is stored in sugars and other organic compounds. It is an endergonic and anaerobic reaction. Critical to the process is chlorophyll‚ the primary photosynthetic pigment in chloroplasts. The chemical equation for photosynthesis is: 36 ATP
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Chromatography Abstract Paper chromatography is one of the methods under chromatography‚ it can use in identifying unknown compounds using known compound and it can also use as a separation technique based on the differences in affinities of components of the mixture to a stationary phase and a mobile phase. In the experiment‚ the stationary phase was the filter paper onto which the dye samples were dropped onto while the mobile phase was the solvent mixture containing ethanol and water which
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Photosynthesis depends on an interaction between two sets of reactions: the light reactions and the Calvin cycle. Chlorophyll and the other molecules responsible for the light reactions are built into the thylakoid membranes. The enzymes that catalyze the Calvin cycle are located in the stroma. Beginning with the absorption of light by chlorophyll‚ the light reactions convert light energy into chemical energy in the form of ATP and NADPH. The ATP provides the energy‚ and the NADPH supplies the electrons
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WHAT IS PHOTOSYNTHESIS? 3 Every day the total amount of energy that is captured through photosynthesis all across our planet is equal to approximately 135 terawatts. Now to put this number into perspective the entire human race consumes approximately 15 terawatts of energy daily. This means that there is 9 times more energy being absorbed every day through photosynthesis‚ as there is energy being consumed by all of mankind (Bridges 2008). In order to fully understand photosynthesis
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Chromatography • • • Separation based on polarity of compounds Two potential phases for a compound to exist in: mobile and stationary Partitioning of compounds between mobile phase and stationary phase occurs: o Compounds that are less polar move more in the mobile phase‚ those that are more polar “stick” more on the stationary phase o These polarity differences cause compounds move at different rates and therefore can be separated 1. Mobile Phase: the phase the moves; can be gas or
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[pic] Photosynthesis Outline Terms and Vocabulary Autotroph Photosynthesis Photon Pigment Chloroplast Reaction Center Electron Donor Molecule Electron Transport System Ferredoxin NADPH RuBP PGA Cuticle Stomata Rubisco Photorespiration C4 Pathway Concepts 1. What are two Adaptations of plant leaves for capturing light? 2. Describe the properties of Light Energy in terms of energy and wavelength. 3. Why are Pigments important for Photosynthesis? 4. What
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Truesdell AP Biology Period 5 2 October 2013 CHAPTER 7 STUDY GUIDE AN OVERVIEW OF PHOTOSYNTHESIS Autotrophs are plants that can feed themselves by making their own food. Photo-autotrophs are organisms that produce organic molecules from inorganic molecules using the energy of light. Producers are when plants make their own organic molecules. A redox reaction is oxidation and reduction. Photosynthesis takes water and carbon dioxide to make glucose and oxygen. Cellular respiration takes glucose
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Figure 1: Affinity chromatography of fumarase with the Ni2+-NTA-agarose column. Extract (9.9 mL) containing yeast (3.76g) in extraction buffer containing 0.1% Igapel CA-630 and protease inhibitors were pumped through Ni2+-NTA-agarose column. Fractions were collected by 1.5 mL portions by use of wash buffer (20.0 mL)‚ imidazole elution buffer (26.3 mL)‚ and wash buffer (10.0 mL)‚ again. Absorption readings were taken for all fractions with a Cary50 set at 280nm. The fumarase activity was determined
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Photosynthesis and the Amount of Light _______________________________________________________________________ I. Introduction Photosynthesis the process where plants use sunlight (energy) to synthesize foods forming the products carbohydrates and water (H20 + CO2 + Light CH2O + O2). For photosynthesis to take place they need water‚ carbon dioxide and light and chloroplasts. Light is absorbed inside he thylakoid
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