Analysis and Separation of Organic Acids in White Wine Using High Performance Liquid Chromatography Atis‚ Arnelson Arwin G. and Gaitos‚ Gerald M. Institute of Chemistry‚ University of the Philippines‚ Diliman‚ Quezon City Performed: December 9‚ 2011 Submitted: December 15‚ 2011 RESULTS AND DISCUSSION solutions containing increasing amounts of the organic acid. These solutions were contained in flasks numbered 5‚ 6‚ 7‚ 8‚ and 9. (Refer to
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Introduction Paper chromatography is a method using a chromatography paper to identify the many mixtures of one simple color. The word chromatography comes from the Greek words‚ "chroma‚" meaning color‚ and "grafein‚" meaning to write. The process of this experiment is to place a colored dot‚ such as marker ink‚ onto the chromatography paper‚ one centimeter away from the triangular tip. Next dip your chromatography paper into the solvent‚ not allowing it to touch the colored dot. Let it sit for
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techniques associated with thin layer and column chromatography. • Determine the effects of the stationary phase in separating a mixture by chromatography. • Determine the effects of the mobile phase in separating a mixture by chromatography. • Conduct a separation using column chromatography. In the first week‚ the students will determine the best solvent system for separating a methylene blue/sodium fluorescein mixture by thin layer chromatography using silica gel and alumina as the stationary
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Chromatography serves mainly as a tool for the examination and separation of mixtures of chemical substances. Chromatography is using a flow of solvent or gas to cause the components of a mixture to migrate differently from a narrow starting point in a specific medium‚ in the case of this experiment‚ filter paper. It is used for the purification and isolation of various substances. There are two phases in chromatography: 1. Stationary Phase – a solid that does not move. In this experiment was the
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Paper chromatography is an important separation technique that depends upon differences in how strongly the dyes are adsorbed onto the paper (stationary phase) and how soluble the dyes are in the developing solvent (mobile phase). In paper chromatography‚ a small amount of the mixture to be separated is placed close to the edge of a piece of paper. The edge of the paper is then immersed in a developing solution. As the developing solution ascends up the paper by capillary action‚ the. components
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Column Chromatography of Plant Pigments Jaybee Balilea‚ Sharmaine Baysic‚ Maria Anjelette Patricia Belen 3Bio-7‚ Department of Biological Sciences‚ University of Santo Tomas‚ Manila‚ Philippines Abstract Column Chromatography is a form of solid-liquid adsorption chromatography and depends on the essential principles as does in thin layer chromatography. It was used in this experiment in separating and analyzing the different components of Capsicum frutescens (siling labuyo) with the use
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How Chromatography Helps The Human Race Chromatography is the separation of a mixture by passing it in solution or suspension or as a vapor (as in gas chromatography) through a medium in which the components move at different rates. “Chromatography is done by making of a mixture move past the solids‚ or across the surface of a solid‚ like paper. The mixture is poured onto a solid surface. As the different components of the liquid run down the solid‚ some of them move more slowly than other. A component
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Gabriel Alizaidy Liquid Chromatography September 14‚ 2009 Objective: To separate the components of unsweetened‚ grape flavored Kool-Aid. Procedure: Using two syringes‚ inject different concentrations of alcohol to extract red and blue dyes‚ and artificial flavoring. Data: Conclusion: Different dyes are shown when separated by different concentrations of isopropyl alcohol. Discussion of Theory: Chromatography‚ resolution and selectivity played major roles in making the experiment work
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Unversity Visca‚ Babay City‚ Leyte Name: Millama‚ Engene O. Course & Year: BS Biotech-2 Lab Schedule: W (1-4) Group 5 Date Performed: Dec. 12‚ 2012 Date Submitted: Jan. 9‚ 2013 Separation of Photosynthetic Pigment by Paper Chromatography I. INTRODUCTION There are different types of components in plant pigments. The most important and abundant chemical pigment found in plants is chlorophyll. This pigment exists in two forms; chlorophyll a and chlorophyll b. Chlorophyll a
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OBJECTIVES: The objective of this experiment was to extract plant leaf pigments and determining them by using the Rf values obtained from the paper chromatography technique. The hypothesis of the experiment was that all of the five listed pigments would be present in the extracted plant leaf according to the Rf values. PROCEDURE/APPARATUS: The equipments used were a 18 x 150 mm test tube with stopper‚ graduated cylinders‚ Erlenmeyer flask‚ mortar and pestle‚ metric ruler‚ tall jar‚ acetone‚ tiny
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