Thin Layer and Column Chromatography of Extracted Total Lipids of Chicken Egg Yolk Aegan Matthew V. Amican‚ Karen Gem B. Ares‚ Ruvie Ann A. Ballester‚ Mark Joseph S. Barcelona‚* Katherine Carmen Isabel G. Calleja‚ Christelle Venus F. Capuno‚ Group 1‚ 2DPH‚ University of Santo Tomas Abstract Lipids are one of the major constituents of foods‚ and are important in our diet for a number of reasons. They are a major source of energy and provide essential lipid nutrients. This experiment determined
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Extraction Important Peaks Concluded Compound C-5 #1 -CH‚ C=O cyclopentanone C-5 #2 -OH‚ -CH 1-hexanol D-4 #1‚2 C=O‚ -CH Cannot conclude Preparative Gas Chromatography was used to separate volatile compounds‚ which can then be identified. The injected sample becomes vaporized and was carried through the mobile column. It is usually carried by an inert gas. The gases were moved through the stationary column. The polarity of the components to the column would cause the separation of the components
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corresponding anthocyanidins using paper chromatography‚ and spectrophotometric analysis procedures. Based on experimental data the blueberries contained the most amount of anthocyanins at 1.92 x 10-6 g‚ followed by blackberries at 1.19 x 10-6 g‚ finally followed by raspberries at 5.84 x 10-7 g. The paper chromatography data also supports the idea that blueberries contained the most anthocyanidins‚ since it contained the most streaks on the chromatography paper. The main conclusion that can be
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Plant Pigment Chromatography VII. Analysis (Questions) 1. What factors are involved in the separation of pigments? Factors that are involved in the separation of pigments can include pigment solubility‚ the attraction between the pigments and paper and the size of each pigment particle. Because of these factors the results were as they were. Beta-carotene traveled the furthest because it forms no hydrogen bonds to the chromatography paper and is slightly soluble in the solvent. Contrastingly
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Ink Chromatography Lab Background Information Paper chromatography is an analytical method that is used for separating and identifying mixtures of substances into their smaller parts. Paper chromatography works because the ink used contains several dyes (pigments) that when place on porous paper are dissolved in a solvent by capillary action. When the pigments (solutes) are dissolved in the solvent (water & alcohol mixture) they move through the paper at different rates depending on their
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CHROMATOGRAPHY LAB Name ______________________ Date ________________ Lab # ________ Partner _____________________ Purpose: To separate a mixture into its components. To learn the technique of chromatography. Materials: filter paper 2.0 ml of vinegar Felt marker pens ruler 400 ml beaker pencil 10 ml graduated cylinder paper clip Water - H2O Safety: Wear goggles and apron during the entire lab. Clean up all materials
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------------------------------------------------- Paper Paper is a thin material produced by pressing together moist fibers‚ typically cellulose pulp derived from wood‚ rags or grasses‚ and drying them into flexible sheets. Paper is a versatile material with many uses. Whilst the most common is for writing and printing upon‚ it is also widely used as a packaging material‚ in many cleaning products‚ in a number of industrial and construction processes‚ and even as a food ingredient – particularly in Asian cultures. Paper‚ and the
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How to Write an Academic Paper Using Method Instead of Madness Jim Janossy 10/31/2005 Writing papers is‚ for many students‚ a difficult‚ trying‚ and intimidating task. This is truly unfortunate because typically at least half of the courses students take in college use paper-writing as a form of academic exercise. Expressing yourself well in writing is often a crucial skill in the professional world. Yet whereas being articulate and knowledgeable in written composition in the workplace is a prized
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Some interesting sites are listed below. Note that some of these sites go into much more depth than is reasonable for this course. http://en.wikipedia.org/wiki/Chromatography http://ull.chemistry.uakron.edu/analytical/Chromatography/ http://orgchem.colorado.edu/hndbksupport/TLC/TLC.html this is for TLC – similar to paper http://users.rcn.com/jkimball.ma.ultranet/BiologyPages/C/Chromatography_paper.html http://jchemed.chem.wisc.edu/JCESoft/Programs/CPL/Sample/modules/paprchrom/paprchromdesc
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History Main article: History of paper Hemp wrapping paper‚ China‚ circa 100 BCE. The oldest known archaeological fragments of the immediate precursor to modern paper date to 2nd century BC in China. The pulp papermaking process is ascribed to Cai Lun‚ a 2nd century AD Han court eunuch.[1] With paper an effective substitute for silk in many applications‚ China could export silk in greater quantity‚ contributing to a Golden Age. Paper spread from China through the Middle East to medieval Europe
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