amber or non-amber‚ and storage temperatures (4‚ 25 and 40°C) on the stability of 12 extemporaneously prepared Phenytoin oral suspension was evaluated. The concentration of Phenytoin oral suspension was evaluated using high performance liquid chromatography (HPLC)‚ in which the retention time of Phenytoin was detected at 6.3 minutes. Phenytoin oral suspension was found to be stable up to two weeks‚ retaining minimal of 76.5% of Phenytoin. Degradation could have taken place as a lower concentration
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(β-sitosterol) and tocopherols (γ-tocopherol). Gas chromatography (GC) was used to determine the fatty acid composition of the triacylglycerol‚ sterol esters and phospholipids where it showed that 80% of the triglyceride composition was trilinolein (31.3–32.2%)‚ oleo dilinolein (31.0–34.0%) and palmitoyl dilinolein (14.9–22.3%). Palmitic acid and oleic acids were the major fatty acids of the
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bodies (IBs) are extracted from these E coli by adding Bugbuster lysis solution and lysozyme. Polyacrylamide gel electrophoresis (SDS-PAGE) is carried out to test the existence of IBs. Afterward‚ IBs are dissolved in 8M urea and poured into IMAC chromatography in peptide purification step. Optimization of each step is tested throughout the experiment. Table 7 below shows the experimental matrix for the experiment.
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Unit 4 Assessment 2-Benzoic Acid Synthesis Synthesis and Investigation of Benzoic Acid Our aims: Create benzoic acid using benzaldehyde and hydrogen peroxide. Then remove some impurities from the benzoic acid crystals. Apparatus: Titration Pipette (25 cm3) Burette (50 cm3) Retort stand Clamp Conical flask (250 cm3) Volumetric flask (250 cm3) and stopper White tile Beakers (250 cm3) Dropping pipette Filter funnel Deionised water Phenolphthalein indicator Volumetric flask
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Amino acids are important building blocks; they play an essential role in the formation of proteins and as intermediates in metabolism. Amino acids can serve as cell signaling molecules and can regulate gene expression. An optimal amount of amino acids is essential for health. There are over 500 amino acids‚ however only 20 are commonly found. Amino acids all have an amine functional group and a carboxylic acid functional group. They differentiate from each other in their side chains. Only 10 of
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EXPERIMENT #4: STATE OF MATTER SLEUTH Calculations To find the molecular weight of the unknown salt: The unknown salt that was assigned is unknown salt in vial #2‚ which was weighed to 0.12 g mass. Initial Volume of NaOH | Final Volume of NaOH | Volume of NaOH needed | 46.6 mL | 34.8 mL | 46.6-34.8=11.8 mL 46.6-34.8=0.0118 L | 1) Number of moles of NaOH used=0.0118 L of NaOH0.1 M of NaOH 1) Number of moles of NaOH used=0.00118 mol 2) The number of moles of NaOH used is equal
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Lab 2: Polar Bonding Student Name: Emerald Dillard Course ID/Section: BIOL 1408-55426 Date: June 13‚ 2015 Answer the questions and report your data in this fillable PDF using the observations and results you recorded in your lab manual while performing the experiments. Save the completed PDF file with your last name and lab number and submit the report as directed by your instructor. (For example: jones_lab2.pdf) Consider the Concepts 1. Explain‚ in one to two complete sentences‚ why a nonpolar
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an enzyme can create different pigments compared to a pigment that is resulted from having no mutations. If enzymes determine the pigment in the eye of Drosophila and we test different Drosophila with different eye pigments and run them through chromatography then there will be a difference in the pigments present between each Drosophila. Procedures: The equipment that is needed to conduct this experiment is a 15x20 cm rectangle of Whatman No. 1 filter paper‚ an etherizer‚ ether‚ a vial of fruit flies
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bp). SDS-PAGE analysis of auto-induced positive clone cell lysate revealed the expression of the cfp-wzb fusion protein (experimental: 44.3 kDa). Using a hexahistidine tag‚ successful fusion protein purification was achieved via nickel affinity chromatography and confirmed by SDS-PAGE. Finally‚ para-nitrophenyl phosphate assay of tyrosine phosphatase activity allowed for determination of wzb kinetic properties such as Km (6.34 mM) and Vmax (0.0644 µmol/min/mg). Gaining an experimental competency in
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source of antioxidant‚ has been demanded due to its biological and physiochemical properties. Therefore‚ there is a need to develop the form of Lycopene supplement. Lycopene was extracted from tomato wastes by using acetone and purified by column chromatography. The extract was characterized by UV-Vis spectrophotometer. An average recovery of 40 mg lycopene / kg tomato wastes was obtained. Lyco-cookies were prepared by using common ingredients containing 3 mg lycopene per serving. Introduction
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