Title: Preparation and Assay of Phenolase and Peroxidase from Sweet and Irish Potato Aim To design and conduct an experiment to demonstrate the presence of enzyme activity in the preparation provided. To examine the effect of the inhibitors provided. To test whether the other phenolic substrates provided can be oxidized by the enzyme preparation. To test for the presence of peroxidase activity in the enzyme preparation. To test the effect of the inhibitor provided on peroxidase activity
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regulators and food producers to protect consumers from natural food toxins‚ consumption of small levels of these materials is unavoidable. The countries across the globe mostly prefer GC-MS and LC-MS (Gas/liquid chromatography-Mass spectrometry) for detecting food toxins. In chromatography‚ the difference in the chemical properties among molecules in a mixture promotes separation of the molecules. On the other hand‚ mass spectrometer captures‚ ionizes‚ accelerates‚ deflects‚ and detects the ionized
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Aspirin Background Aspirin Aspirin‚ or acetylsalicylic acid (ASA) is a salicylate drug‚ and is generally used as an analgesic (something that relieves pain without producing anaesthesia or loss of consciousness) for minor aches and pains‚ to reduce fever (an antipyretic)‚ and also as an anti-inflammatory drug. Aspirin works by prohibiting biological substances such as prostaglandins. Many different prostaglandins exist in the human body each serving a plethora of physical functions.
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Vitiligo Vitiligo is a skin condition‚ and sometimes is referred to as the Michael Jackson Disease. It is a skin condition in which there is a loss of pigment from areas of skin. That then results in irregular white patches of skin. Vitiligo occurs when the melanin forming cells‚ also called melanocytes fail to produce melanin. Melanin is the dark pigment that is in the epidermis that gives us our skin color. When the melanocytes fail to produce melanin‚ white patches of skin appear on the skin. Although
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oleracea and Musa acuminata‚ which use different wavelengths of light to display various pigments of color. The light energy for photosynthesis calls for an array of light to be absorbed by the plants. In this experiment‚ the different wavelengths of light can be traced and graphed by extracting chlorophyll from these two plants and placing them into a spectrophotometer‚ which identifies what wavelengths of light a pigment absorbs. It was found that Musa acuminata‚ commonly referred to as banana palm‚ showed
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of cellular pigment. Albinism is a form of hypopigmentary congenital disorder‚ characterized by a partial hypomelanism or total lack of melanin pigment in the eyes‚ skin‚ and hair. Albinism results from inheritance of recessive alleles. Also another term used for albinism is an albino. Albinism is hereditary; it is not an infectious disease and cannot be transmitted through contact blood transfusions or other rectors. Many types of albinism exist‚ all of which involve lack of pigment in varying degrees
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Pure Appl. Chem.‚ Vol. 78‚ No. 8‚ pp. 1477–1491‚ 2006. doi:10.1351/pac200678081477 © 2006 IUPAC Carotenoids and other pigments as natural colorants* Alan Mortensen Chr. Hansen A/S‚ Color Research‚ Development and Application‚ Bøge Allé 10-12‚ DK-2970 Hørsholm‚ Denmark Abstract: Food colorants may be classified into synthetic‚ nature-identical‚ inorganic‚ and natural colorants. Natural colorants for food are made from renewable sources. Most often‚ the colorants are extracted from plant
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Stereochemistry of Dihydroxylation Reactions Question: Can the stereochemical outcome of dihydroxylation reactions be determined by thin layer chromatography? Overview: In this experiment‚ you will perform two reactions that transform alkenes into diols. While similar in functional group transformation‚ these reactions may lead to stereochemically different products. The two possible products are diastereomers of one another‚ and therefore have different physical chracteristics. After
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supernatant is to be removed and labeled accordingly in a separate vessel. Supernatant is the liquid above the sediment after a solution has been run through a centrifuge. The now centrifuged solution should be ran through a device that achieves column chromatography‚ such as an automated fraction collector. These devices sort a solution based on particle size. Other methods to extract proteins (which can be many different
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Hypothesis Organic solvent and high temperature will destroy the cell membrane and make it permeable to the red pigment. The higher the concentration of the alcohol‚ the greater will be this effect. Principle Beetroot is the material used in this experiment to demonstrate the effects of high temperature and chemicals on the permeability of the cell membranes. Beetroot contains a red pigment called betalain‚ which is located in the large central vacuole of the beetroot cells. The vacuole is enclosed
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