homogenate was made by adding the buffer to the liver of the quail after one hour centrifuge at 12300 RPM materializing at 4°C. Precipitation protein was working by ammonium sulfate; it loaded directly to column 2’‚ 5’ ADP Sepharose 4B affinity chromatography‚ which has been a high affinity to NADP+ substrate. The flow rate of elution was 23 ml/hr. Taking 18 fractions‚ checking activity for each fraction at 340 nm. The activity found in fraction tube six to fourteen. This process was materializing
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for epimers and isobars Iltaf Shah1‚ Ricky James1‚ James Barker2‚ Andrea Petroczi1 and Declan P Naughton1* Abstract Background: Recently‚ the accuracies of many commercially available immunoassays for Vitamin D have been questioned. Liquid chromatography tandem mass spectrometry (LC- MS/MS) has been shown to facilitate accurate separation and quantification of the major circulating metabolite 25-hydroxyvitamin-D3 (25OHD3) and 25hydroxyvitamin-D2 (25OHD2) collectively termed as 25OHD. However
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Manufacturing of Aerogel Aerogel was found by Steven Kistler in 1931‚ but its application cannot be in the market and consumer because there is no item that can use the aerogel. The 1980s the aerogel have a place to be a raw material in existing and new product after they discover a new research about aerogel. They have been considered as a new development material that better than polyurethane foam in refrigerators‚ and as a heat insulator for windows. Aerogel is produced by removing all the
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this experiment‚ a mixture of two compounds‚ cyclohexane and toluene‚ was separated into fractions by the techniques of simple and fractional distillation. The individual fractions that were gathered from the distillation were analyzed using gas chromatography (GC) and used to compare the efficiencies of the two different distillation techniques. The ultimate goal of this experiment was to determine whether simple or fractional distillation was the more efficient means of separating volatile compounds
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Using this reaction and its product‚ a redox experiment was performed to familiarize students with important organic chemistry laboratory techniques including proper mixing in an oxidation reaction‚ extraction‚ infrared spectroscopy‚ and gas chromatography. Reactants cyclohexanol and sodium hypochlorite (NaOCl) undergo a redox reaction and when combined with the catalyst acetic acid (HOAc) and solvent water (H2O)‚ form the product cyclohexanone along with sodium chloride (NaCl) and water. In the
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The product was then purified by flash chromatography and analyzed by IR‚ 1H NMR and 13C NMR. The synthesis of piperonylontitrile was successful. There was a yield of a pale yellow crystal of 36%. The average recovery is 51% with there being a range from 0-94 %1. A reason for a smaller yield than
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Chemistry of Spices This page intentionally left blank Chemistry of Spices Edited by Villupanoor A. Parthasarathy Indian Institute of Spices Research Calicut‚ Kerala‚ India Bhageerathy Chempakam Indian Institute of Spices Research Calicut‚ Kerala‚ India and T. John Zachariah Indian Institute of Spices Research Calicut‚ Kerala‚ India CABI is a trading name of CAB International CABI Head Office Nosworthy Way Wallingford Oxfordshire OX10 8DE UK Tel: +44 (0)1491 832111 Fax:
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binds the nucleoside inhibitor site. When it binds this site‚ it stabilizes the inactive T state and blocks the catalytic site which needs to be open for enzyme activity to occur. The glycogen phosphorylase b was purified with hydrophobic column chromatography and the concentration was determined with a Bradford Assay. The kinetics of glycogen phosphorylase b were studied by finding a molar extinction coefficient‚ 0.1617 mM cm-1 A-1‚ for
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MIXTURES‚ SOLUTIONS‚ SUSPENSIONS AND COLLOIDS MIXTURES A mixture is a combination made up of two or more different substances which are mixed but are not chemically bonded. There are also types of mixtures such as homogeneous mixtures and heterogeneous mixtures. SOLUTIONS A solution is defined as a homogeneous mixture composed of a solute; a substance dissolved into another substance known as a solvent. They can also be defined as groups of molecules that are mixed up completely in even
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Food Chemistry journal homepage: www.elsevier.com/locate/foodchem Analytical Methods Determination of volatile N-nitrosamines in meat products by microwave-assisted extraction coupled with dispersive micro solid-phase extraction and gas chromatography – Chemical ionisation mass spectrometry Man-Chun Huang‚ Hsin-Chang Chen‚ Ssu-Chieh Fu‚ Wang-Hsien Ding ⇑ Department of Chemistry‚ National Central University‚ Chung-Li 320‚ Taiwan a r t i c l e i n f o Article history: Received 21
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