Experiment 10 Thin Layer Chromatography Purpose! To identify how many components are in a mixture and the identity of a compound in a mixture by comparing the Rf of a known compound with the Rf of an unknown compound. Intodection. In this experiment the thin-layer chromatography the (TLC) will be used to determine the composition of various over-the-counter analgesic. We will be given two commercially prepared TLC plates with a flexible backing and a silica-gel coating with a fluorescent
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Matter Lab Perform Paper Chromatography Lab Turn-in: 1) Changes: Physical or Chemical Lab - Completed Procedure / Observations‚ questions‚ and a written description of most important concept that you learned in the discussion section. 2) Special Physical Properties of Matter Lab – Completed Procedure / Observations‚ questions‚ and a written description of most important concept that you learned in the discussion section. 3) Paper Chromatography Lab – Completed Procedure / Observations
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composition that was found out in the analysis. The % composition of the yield was found out by using the % composition of the products and a gas chromatography by measuring the peak areas for each gas chromatogram. The operations used in this lab in their order of usage were: Simple distillation‚ mixing‚ washing liquids‚ drying liquids‚ gas chromatography. 2. Procedure The volume of 20 mmol of 2-methylcyclohexanol was calculated and transferred into a round-bottom flask. Then 0.7 mL of 85 % phosphoric
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Cited: 1. Armstrong G.A.‚ J.E. Hearst. 1996. Genetics and molecular biology of carotenoid pigment biosynthesis. Journal of the Federation of American Societies for Experimental Biology.10: 228-237. 2 3. Bohlmann J.‚ G. Meyer-Gauen‚ R. Croteau. 1998. Plant terpenoid synthases: Molecular biology and phylogenetic analysis. National Academy of
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such as activity and protein assay were employed to determine the presence and purity of LDH. The cells were initially disrupted and proteins were solubilized. LDH was purified from the ammonium sulfate precipitated protein mixture by affinity chromatography and its activity was studied by spectrophotometric determination of NADH at 340 nm. From Pierce BCA assay of crude homogenate‚ initial protein concentration was shown to be 100 mg/ml. The final protein concentration of the pooled affinity sample
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Beam Chopping corrects for flame emission but not for scattering. Can distinguish the signal of the flame from the atomic line at the same wavelength. The higher the absorbance the higher the transmittance. Intro to IR Near IR- (0.78 to 2.8 micrometers) Mid IR- (2.5- 50 micrometers) Far IR- (50-1000 micrometers) Most common region used is Mid IR btwn 2.5- 15 micrometers Mid IR: based on diffracting gratings but now current instrumentation are of Fourier transform type Far-IR: useful
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extraction with 10 mM formic acid solution and precipitation at low temperature by centrifugation. The utilise of C-18 reversed-phase columns were slightly more polar performed to achieve a better separation of acrylamide. Eluent system of liquid chromatography used a time programme gradient in order to obtain a better selectivity. The mobile phase was a mixture of 10 mM formic acid and methanol. The performance parameters of precision‚ accuracy and recovery were conducted to assure that the analytical
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Colorado Northwestern Community College Science of Biology Mrs. Farrow Lab 3 – Slime Time Submitted by Chase Kenemer 22 February 2015 Abstract Polar solvents dissolve‚ or pick-up‚ polar substances and non-polar solvents dissolve‚ or pick-up‚ non-polar substances. In the conducted experiment‚ the polarity of molecules and their properties are explored. The results of using two solvents on both polar and non-polar inks‚ further verify this to be true. The student conducted the experiment
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material (recycled). Heat energy is produced as a by-product b/c so it is used to make steam in the exchanger saves money + energy 4) The product is analysed to monitor purity. Products made from synthesis Basic organics Petrochemicals Pigments‚ paint‚ dyes Pharmaceutical Speciality chemicals Innovations in green chemistry 1B Regulations are put in place for the storing and transporting of chemicals. Hazard symbols and sealed containers prevent harm from
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which is glycerol is filtered off by a separation process and an impure biodiesel will be obtained. The biodiesel obtained is still contained a little amount of glycerol and other impurities. Hence‚ to get pure biodiesel‚ high-performance liquid chromatography (HLPC) is used to further purify the biodiesel. The biodiesel yield can be calculated relative to the initial amount of Pongomia Pinnata oil by weight and the biodiesel purity is determined
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