I. Experiment 3: SEPARATION TECHNIQUES II. Objectives: * To learn different methods and techniques of separating components of mixtures * To appreciate the importance of such separation techniques * To apply different methods and techniques in separating components of mixtures III. Results and Observations Separation of components with different solubilities * AMMONIUM CHLORIDE‚ SODIUM CHLORIDE‚ SAND the wt. of mixture with evaporating dish after 1st heating is?? g. And after
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The poly-A tail is in the 3’UTR. The stop codon is always upstream of the terminator More than one of a-d are false 2. Immunoprecipitation is based on a similar principle as: a) b) c) d) e) SDS-PAGE Ion exchange chromatography Size exclusion chromatography Affinity chromatography PCR 3. How is a Western blot different from a Southern Blot‚ as we covered in class? a) b) c) d) e) f) A Western blot uses a gel. A Western blot uses a membrane. A Western blot uses an antibody. A Western blot uses
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(e.g.‚ ClO2-‚ and ClO-) in biological fluids. These biomarkers can be used to indirectly measure chlorine dioxide exposure. The concentration of residual chlorite ion in vegetables and eggs treated with sodium chlorite was determined by UV-ion chromatography (Suzuki et al. 1997). Sodium chlorite was extracted with water and cleaned-up using C18 cartridge. The detection
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flocculation of the green alga Botryococcus braunii. Lett Appl Microbiol 1998;27:14 – 8. Li HB‚ Chen F‚ Zhang TY‚ Yang FQ‚ Xu GQ. Preparative isolation and purification of lutein from the microalga Chlorella vulgaris by high-speed counter-current chromatography. J Chromatogr A 2001;905:151 – 5. 514 E. Molina Grima et al. / Biotechnology Advances 20 (2003) 491–515 Lim GB‚ Lee SY‚ Lee EK‚ Haam SJ‚ Kim WS. Separation of astaxanthin from red yeast Phaffia rhodozyma by supercritical carbon dioxide extraction
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Today in lab I will separate mixtures of compounds into their constituent components using chromatography paper and an eluting solvent of salt water as well as rubbing alcohol. Materials and Methods: In this lab using the bag of FD&C dyes‚ bag of M&M’s‚ 2 packs of Kool-Aid ‚ and a pack of Clover Valley store brand food coloring I am going to place a drop of each dye at the bottom of the chromatography paper. For the experiment with the rubbing alcohol I also placed a drop of each dye‚ but used
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use Gas Chromatography to distinguish between two enantiomers of carvone from caraway oil and spearment oil and to find the 2 carvone’s optical activity as well as percent carvone in spearment and caraway oil. It was found that S-carvone had an optical activity of 0.0047 and R-carvone had an optical activity of 0.516 and that spearment oil is 59% carvone and caraway oil is 100% carvone. Backround: Gas Chromatography separates organic samples much in the same way as column chromatography. The only
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Name: Date: Course: Lesson: Unit: Chromatography Lab INTRODUCTION: In 2 to 3 complete sentences‚ explain the purpose of this lab – what are you going to learn in this lab? (2 points) HYPOTHESIS: Answer the following in complete sentences: (2 points) 1. List one extensive and one intensive property of marker ink. 2. Define mixture. MATERIALS: coffee filter Tape rubbing alcohol water 3 different color magic markers (not permanent) 3 identical tall‚ narrow
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Partition equilibrium of a solute between two immiscible solvents Two-dimensional thin-layer chromatography 2 2 3 6 Chapter 65 Quantitative methods of analysis Detection of end point in acid-alkali titration 8 Chapter 66 Instrumental analytical methods More about infrared spectroscopy More about mass spectrometry 12 15 Chapter 67 Contribution of analytical chemistry to our society Gas chromatography-mass spectrometry 32 PART XV ANALYTICAL CHEMISTRY Chapter 63 Detecting the presence
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Briefing Thin-layer chromatography (TLC) is an extremely valuable analytical technique. It provides a rapid separation of compounds‚ and gives an indication of the number and nature of the components of a mixture. TLC can also be used to identify compounds by comparison with known samples‚ to check the purity of a compound‚ or to monitor the progress of a reaction‚ an extraction‚ or a purification procedure. This experiment will introduce you to the mechanics of TLC‚ and the chemical principles
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Forensic Science: Analytical Techniques Unit 6 Project Student: Steve Gregory Kaplan University CJ385-01 Professor: Al Luckas Forensic Chemistry and Trace Evidence Analysis Author S. Gregory October 31‚ 2011 Forensic scientists are governed by a process that is known as the scientific method. The scientific method adheres to strict guidelines which will ensure the careful and systematic collection‚ organization‚ and analysis of
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