References: 1) E. Stahl‚ Thin Layer Chromatography‚ Springer-Verlag‚ New York‚ 1969 2) Camag Scientific‚ Inc.‚ 515 Cornelius Harnett Drive‚ Wilmington‚ NC 28401‚ phone 800-3343909‚ fax 919-343-1834 3) Desaga GmbH‚ P.O. Box 101969‚ D-69009 Heidelberg‚ Germany‚ phone 0-62-21-83590‚ fax 0-62-21-840887
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Column and Thin Layer Chromatography Beverly Abstract: Plant pigments were separated and concentrated from a crude spinach extract through the use of column chromatography and an eluatropic series of hexanes‚ hexane/acetone‚ and methanol. The pigments were analyzed using thin layer chromatography with a 30% ethyl acetate/hexane developing solvent. Introduction: Chromatography is a technique used to separate a mixture of two or more components based on
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Thin Layer Chromatography of the Unknown Analgesic Jessica Bajao*‚ Phoebe Abalos‚ Kevin Antiga‚ Carmelus Aseneta 3-Biology 2 College of Science‚ University of Santo Tomas‚ Manila‚ Philippines Abstract The group used five different analgesics in this experiment: aspirin‚ acetaminophen‚ ibuprofen‚ caffeine‚ and mefenamic acid. Six analgesics were spotted on the TLC plate including the unknown. After the development of the TLC plate‚ it was placed under ultraviolet light for the spots resulted
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Experiment 4 – Liquid Phase Chromatography I. Objectives This experiment’s goal is to explore one-dimensional and two-dimensional paper chromatography. II. Schematic Diagram of the Procedure PAPER CHROMATOGRAPHY Wash leaves‚ cut them into smaller pieces; in a mortar macerate them in circular motion Add 8mL ethyl alcohol to extract pigments‚ continue macerating until finely grounded Transfer extract to evaporating dish‚ allow to conc‚ don’t let extract to dry out Concentration
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distillation and gas chromatography based on their difference in boiling points. The boiling point of hexane is 69 degrees Celsius and the boiling point of toluene is 110 degrees Celsius. Three fractions were collected for both simple and fractional distillation. The first fraction was hexane since it had the lower boiling point. The second fraction was a mixture of toluene and hexane. The third fraction was toluene since it had the high boiling point of 110 degrees Celsius. Gas chromatography was also preformed
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Gas Chromatography Analysis of Product Mixtures Gas Chromatography Guidelines‚ Handout. Introduction Gas chromatography is a technique used to analyze chemical compounds that can be vaporized and separated in a gas phase column. Once separated‚ the analyzed substance is passed through a detector and data is obtained. The samples that we are going to analyze are: the EtOAc from Simple distillation‚ the Fraction 1‚ Fraction 2‚ and Fraction 3 from the Fractional Distillation. Experimental Procedure
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CHEM 3152-003 Experiment 2: Fractional Distillation and Gas Chromatography Introduction: The purpose of this experiment was to separate a mixture of ethyl acetate (EtOAc) and toluene by using the method of fractional distillation and to analyze the fractions using gas chromatography (GC). ethyl acetate toluene Distillation is a method known as separating or purifying a liquid or mixture by vaporization and condensation. In a simple distillation‚ the liquids being separated boil below 150 0C
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Candy Chromatography: What makes those colors? The point of doing this project is to use paper chromatography to see which dyes are used in the coatings of your favorite colored candies. Chromatography is a technique for separating mixtures. Chromatography can be used to separate specific components from a complex mixture‚ based on molecular size or other chemical properties. It can also be used to identify chemicals‚ for example crime scene samples like blood‚ drugs‚ or explosive residue. Highly
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Purpose 1. To prepare a dry-packed sample of product mixture of ferrocene and Acetylferrocene 2. To separate and purify the components in the product mixture by column chromatography. 3. To check the purity of the components by thin-layer chromatography (TLC). 4. To calculate the yield of acetylferrocene and the percent recovery of unreacted ferrocene. Table of Quantity showing various physical properties Type of substance Molecular Formula Molecular Weight (g/mol) Density(g/cm3) M.P
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The purpose of column chromatography is to separate one compound from another by the differing polarities in the compound. This technique is important in organic chemistry because certain experiments may require the separation of compounds in solution to be used for chemical synthesis and/or analysis of a particular desired product by isolating it. Fluorene is to be separated from 9-fluorenone using the technique of column chromatography. The success of separation and purity is determined with analyzation
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