Community Outreach & Education Program CHROMATOGRAPHY (Adapted from: Forensic Science Activities. University of Colorado Boulder Hughes Initiative. .) DESCRIPTION: Students will use paper chromatography to separate ink molecules and identify the pen used on an unknown sample of handwriting. Students will graph and analyze data they collect using paper chromatography. PURPOSE/GOAL: Students will be able to: • Gain understanding of the purpose of chromatography. • Measure and graph pigment separation
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Chromatography Abstract Paper chromatography is one of the methods under chromatography‚ it can use in identifying unknown compounds using known compound and it can also use as a separation technique based on the differences in affinities of components of the mixture to a stationary phase and a mobile phase. In the experiment‚ the stationary phase was the filter paper onto which the dye samples were dropped onto while the mobile phase was the solvent mixture containing ethanol and water which
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Natural dyes are dyes or colorants derived from plants‚ invertebrates‚a or minerals. The majority of natural dyes are vegetable dyes from plant sources – roots‚ berries‚ bark‚ leaves‚ and wood — and other organic sources such as fungi and lichens. Archaeologists have found evidence of textile dyeing dating back to the Neolithic period. In China‚ dyeing with plants‚ barks and insects has been traced back more than 5‚000 years.[1] The essential process of dyeing changed little over time. Typically
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techniques associated with thin layer and column chromatography. • Determine the effects of the stationary phase in separating a mixture by chromatography. • Determine the effects of the mobile phase in separating a mixture by chromatography. • Conduct a separation using column chromatography. In the first week‚ the students will determine the best solvent system for separating a methylene blue/sodium fluorescein mixture by thin layer chromatography using silica gel and alumina as the stationary
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Analysis of Food Colorings by Paper Chromatography Introduction Paper Chromatography one method for testing the purity of compounds and identifying substances. This is a useful technique because it is relatively quick and requires small quantities of material. Separations in paper chromatography involve the same principles as those in thin layer chromatography. The substances are distributed between a stationary phase and a mobile phase. The stationary phase is usually
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to dye 3. 1 package RIT powder dye or 1/2 bottle RIT liquid dye 4. Rubber bands‚ string‚ or 2-inch wide strips of cloth for tying Instructions: 1. Gather the shirt into accordion-like folds‚ then fold the gathered fabric in half. Band tightly with cloth strips every 2 or 3 inches. 2. Before starting to dye‚ put on rubber gloves and prepare the dye bath. Dissolve 1 package RIT powder dye or 1/2 cup RIT liquid dye in about 2 gallons very hot water. For dark or bright colors‚ prepare dye bath
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Candy Chromatography Background info: Paper chromatography is a logical technique used to separate works of a solution. Three examples of how we apply this technique to real-life would be: contaneminants in water‚ separation of plant pigmentation‚ and analysis of narcotics. Source: http://www.discoveriesinmedicine.com/Bar-Cod/Chromatography.html#b Purpose: To find out why candies are different colors. * Materials: Candy with a colored coating‚ like Skittles® or M&Ms® (4 different
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PAPER and COLUMN CHROMATOGRAPHY REPORT 1. 2. The unknown code is an amino acid‚ Glycine. To justify the reason is because the retention factor is the same exact number as the Glycine. The data of the unknown shows everything to be exact with the data of the Glycine 3. The mobile phase is the more polar during the capillary action of the experiment. As soon as the paper touches the mobile phase‚ the solvent rises to the amino acids. This is where you can find polarity of the amino acids.
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The earliest surviving examples of pre-Colombian tie-dye in Peru date from 500 to 800A.D. Their designs include small circles and lines‚ with bright colors including red‚ yellow‚ blue‚ and green.[4] Shibori includes a form of tie-dye that originated in Japan. It has been practiced there since at least the eighth century. Shibori includes a number of labor-intensive resist techniques including stitching elaborate patterns and tightly gathering the stitching before dyeing‚ forming intricate designs
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GAS CHROMATOGRAPHY EXPERIMENT The purpose of this experiment is for the student: 1) to learn the general theoretical aspects of gas chromatography as a separation method‚ 2) to learn how to operate gas chromatographs specific to COD‚ 3) to become familiar with using the gas chromatograph (GC) to qualitatively identify components of mixtures‚ 4) to be introduced to and to interpret the quantitative data available via gas chromatography‚ 5) to gain insight into how the GC technique is used
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