CHM 510 LABORATORY REPORT Experiment 1: Gas Chromatography (GC): Optimization of Flow Rate and Column Temperature Name: AFIQ B. ANWAR Student No.: 2012621072 (AS2253A) Date of experiment: Date of report submission: Lecturer’s Name: PN. HALIZA Gas Chromatography (GC): Optimization of Flow Rate and Column Temperature INTRODUCTION The main purpose of the experiment is to investigate the effects of column temperature and flow rate on the separation of methyl esters compounds
Premium Chromatography Gas chromatography
Colouration of Silk with Natural Dyes Dr. Rajashree Phukon‚ Registration ID- AB/565 ‚ Assist. Prof.‚ Sibsagar Girls’ College‚ P.O.Sivasagar‚ Assam‚ Pin-785640‚ e-mail ID:rajashreephukon@yahoo.com and Mr Diganta Kr. Borah‚ Research Scholar‚ Department of Economics‚ Dibrugarh University‚ Assam‚ India‚ e-mail ID: digoborah@yahoo.in. ABSTRACT The study was undertaken with an aim to develop the dyeing conditions of four different natural dyes which are easily available
Premium Dye
Gas chromatography (GC) is a chromatography technique where the separation of individual components (analytes) from a sample relies on their differing distribution between a mobile and stationary phase. The mobile phase carries the analytes through the stationary phase. In GC‚ it’s an inert gas (usually helium or nitrogen). The gas must be inert‚ so it won’t react with the sample to give a false reading. The stationary phase is a substance fixed in place to which the sample adsorbs because
Premium Temperature Thermodynamics Gas
SINGAPORE Chemical Engineering Process Laboratory II Experiment B2 Chromatography for Protein Purification Name Matric No. Group : : : Date of Expt. : GRADE : A. Learning objectives 1. 2. 3. 4. Establish chromatographic assay to determine protein concentrations in a mixture. Appreciate the importance of resolution in protein chromatography. Understand the tension between purity and yield in protein chromatography. Understand the importance of mass balance closure in protein purification
Premium Chromatography High performance liquid chromatography Protein
Abstract Finding the melting point of an organic substance is a practical and efficient way for scientists to identify an unknown substance or determine a known substance’s level of purity. When organic substances are mixed together in varying degrees they take on a melting characteristic that is lower and broader than in its pure form. This property was manipulated in the lab to observe the various melting points of Naphthalene and Biphenyl when the percentage of composition was altered. A
Premium Liquid Temperature Solid
Skin Dye made the dye bath turn into a light yellow color; However‚ this color did not look like the color of the onion skin. The Carrot Tops Dye had not changed the color of the dyed bath but stayed clear throughout the experiment. On the other hand‚ in Picture 2‚ the Zinger tea and Chamomile tea had deeper color in their dyed baths: orange and dark red‚ respectively. None of the yarn samples were the same color as the dyed baths‚ except for the yarn sample that was dyed with the onion dye. As shown
Premium Water Recyclable materials Recycling
Data and Conclusions: The purpose of this experiment was to learn how to use distillation and gas chromatography to separate and identify different compounds from a given mixture. There are several kinds of distillation methods. However‚ the method that we used in this experiment was fractional distillation. This method is used when trying to separate two different volatile compounds whose boiling points differ by 40-50°C or more. If the boiling points are too close‚ this method
Premium Distillation Evaporation Water
The purpose of this lab was to use gas chromatography to determine the relative amounts of the three components of gas products from the dehydration reaction of 1- and 2-Butanol with sulfuric acid and the dehydrobromination reaction of 1- and 2-Bromobutane with potassium tert-butoxide. E1 reactions are two-step unimolecular reactions. The first step of an E1 reaction occurs when a leaving group‚ which consists of a weak base‚ leaves an organic compound‚ leaving an intermediate carbocation. In the
Premium Alcohol Oxygen Chemistry
Chromatography • • • Separation based on polarity of compounds Two potential phases for a compound to exist in: mobile and stationary Partitioning of compounds between mobile phase and stationary phase occurs: o Compounds that are less polar move more in the mobile phase‚ those that are more polar “stick” more on the stationary phase o These polarity differences cause compounds move at different rates and therefore can be separated 1. Mobile Phase: the phase the moves; can be gas or
Premium Chromatography Solubility Gas
9.1 Orbitals and Theories of Chemical Bonding 1. Which one of the statements concerning valence bond (VB) and molecular orbital (MO) bond theories is correct? a) MO theory predicts that electrons are localized between pairs of atoms. b) In VB theory‚ bonding electrons are delocalized over the molecule. c) MO theory accurately describes bonding in O2 and NO‚ VB theory does not. d) VB theory can describe molecular bonding in excited states. e) MO theory is used to accurately predict
Premium Chemical bond Atom Electron configuration