Lesson Learning Outcomes Upon completion of this lecture‚ students should be able to: understand the structures and properties of amino acids know the groups of amino acid What are amino acids? Amino acids are molecules that when combined with each other proteins. Amino acids contain a central tetrahedral carbon atom (α-carbon) amine group‚ carboxyl group‚ R-side chain The R-side chain determines the different amino acids There are 20 common amino acids Amino acids can join via peptide
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Organic Chemistry 12A Professor Alston October 28‚ 2014 Isolation of Chlorophyll and Carotenoid Pigments from Spinach Purpose The purpose of this experiment was to isolate ß-carotene‚ chlorophyll-A‚ and chlorophyll-B from spinach using column chromatography. Spinach was dehydrated using ethanol‚ and the pigments were extracted with dichloromethane. The spinach extracts were dried using CaCl2. Then‚ the solid pigments were run through a column using a non-polar solvent‚ hexane. The polar absorbent
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Tamilnadu. E-Mail: velmpharm24@gmail.com A precise‚ accurate and rapid RP-HPLC method has been developed and validated for the simultaneous estimation of Tamsulosin Hydrochloride and Finasteride in tablet dosage form. Chromatography was carried on RP – 18e Hiber RT (250 × 4.6) column using Water:Methanol (30:70%) v/v as mobile phase at a flow rate 0.7 ml/min and the effluent was monitored at 225 nm. The retention times of Tamsulosin Hydrochloride and Finasteride were 5.4
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raise by less than 0.06% as he is larger than an average male. 6. Name three methods for drug screening describe the advantages and disadvantages of each. Name Advantages Disadvantages Immunoassays High sensitivity Not 100% specific Thin Layer Chromatography Can identify hundreds of compounds in one run and is inexpensive Labour intensive and highly technical Ultraviolet-Visible Spectrophotometry Reliable compound recognition Cannot identify less
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Experiment 10 Thin Layer Chromatography Purpose! To identify how many components are in a mixture and the identity of a compound in a mixture by comparing the Rf of a known compound with the Rf of an unknown compound. Intodection. In this experiment the thin-layer chromatography the (TLC) will be used to determine the composition of various over-the-counter analgesic. We will be given two commercially prepared TLC plates with a flexible backing and a silica-gel coating with a fluorescent
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Matter Lab Perform Paper Chromatography Lab Turn-in: 1) Changes: Physical or Chemical Lab - Completed Procedure / Observations‚ questions‚ and a written description of most important concept that you learned in the discussion section. 2) Special Physical Properties of Matter Lab – Completed Procedure / Observations‚ questions‚ and a written description of most important concept that you learned in the discussion section. 3) Paper Chromatography Lab – Completed Procedure / Observations
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composition that was found out in the analysis. The % composition of the yield was found out by using the % composition of the products and a gas chromatography by measuring the peak areas for each gas chromatogram. The operations used in this lab in their order of usage were: Simple distillation‚ mixing‚ washing liquids‚ drying liquids‚ gas chromatography. 2. Procedure The volume of 20 mmol of 2-methylcyclohexanol was calculated and transferred into a round-bottom flask. Then 0.7 mL of 85 % phosphoric
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such as activity and protein assay were employed to determine the presence and purity of LDH. The cells were initially disrupted and proteins were solubilized. LDH was purified from the ammonium sulfate precipitated protein mixture by affinity chromatography and its activity was studied by spectrophotometric determination of NADH at 340 nm. From Pierce BCA assay of crude homogenate‚ initial protein concentration was shown to be 100 mg/ml. The final protein concentration of the pooled affinity sample
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Beam Chopping corrects for flame emission but not for scattering. Can distinguish the signal of the flame from the atomic line at the same wavelength. The higher the absorbance the higher the transmittance. Intro to IR Near IR- (0.78 to 2.8 micrometers) Mid IR- (2.5- 50 micrometers) Far IR- (50-1000 micrometers) Most common region used is Mid IR btwn 2.5- 15 micrometers Mid IR: based on diffracting gratings but now current instrumentation are of Fourier transform type Far-IR: useful
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extraction with 10 mM formic acid solution and precipitation at low temperature by centrifugation. The utilise of C-18 reversed-phase columns were slightly more polar performed to achieve a better separation of acrylamide. Eluent system of liquid chromatography used a time programme gradient in order to obtain a better selectivity. The mobile phase was a mixture of 10 mM formic acid and methanol. The performance parameters of precision‚ accuracy and recovery were conducted to assure that the analytical
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