validation for paracetamol drug includes the parameters such as accuracy‚ precision‚ selectivity‚ sensitivity‚ reproducibility and sensitivity. All this validation methods are applicable to bio-analytical methods such as Gas Chromatography and High Performance Liquid Chromatography. Unfortunately the most undesirable feature of drugs containing the DHP (dihydropyridine) group is high sensitivity to light .Such drugs are regarded as photo labile. Therefore the photo stability testing has become an important
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like red and blue are seemed to be absorbed. If blue and red wavelengths have more absorbance‚ the green doesn’t have a high absorbance. This lab is useful to help demonstrate the existence of various leaf pigments by using the process of paper chromatography.
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The process to determine a protein’s structure starts with the purification of the desired protein. A protein must be placed in an environment with specific conditions that will enable it to be isolated from impurities; different techniques of chromatography are extremely useful in these situations. Purification techniques are utilized by crystallographers because pure proteins must be crystallized in order to determine structure. If proteins crystallize with impurities‚ it can ultimately affect crystal
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1. In table format‚ provide the Rf values you recorded for each of the 3 compounds in Part I. List compound on one axis of your table and solvent system on the other. If multiple spots were present for a compound‚ give Rf value for each component and state if the component was major or minor. | 100% hexane | 25% EtOAc in hexane | 10% EtOAc in hexane | Fluorene | 0.34 cm | 0.94 cm | 0.67 cm | 9 - fluorenol | 0.00 cm | 0.49 cm | 0.05 cm | 9 - fluorenone | 0.00 cm | 0.69 cm | 0.21 cm |
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/50 Lab 9 Report Template: Acid Catalyzed Dehydration of 2-Methylcyclohexanol Abstract In Experiment 9‚ distillation was used to carry out the dehydration of 2-methylcyclohexanol using 4:1 mixture of phosphoric and sulfuric acid. Gas Chromatography was then used to measure the products. Two products were formed. The theoretical yield of the alkene mixture was 3.914 g. The actual yield of the mixture was 0.73 g. The percent yield for the reaction was 18.65%. Peak A had an area of 0.3 mm2
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of the GFP protein. This indicates that the molecule responsible for the observed green trait was protein. The transformation efficiency was found to be 793.75 transformants per microgram. The protein was filtered out of the cell and column chromatography was used as a means to isolate the protein by taking advantage of its hydrophobic properties. This was performed by washing the column containing the GFP protein amongst other proteins with buffers varying in salt concentrations. As expected
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Urine Testing for Drugs of Abuse U. S. DEPARTMENT OF HEALTH AND HUMAN SERVICES • Public Health Service • Alcohol‚ Drug Abuse‚ and Mental Health Administration Urine Testing for Drugs of Abuse Editors: Richard L. Hawks‚ Ph.D. C. Nora Chiang‚ Ph.D. Division of Preclinical Research National Institute on Drug Abuse NIDA Research Monograph 73 1986 DEPARTMENT OF HEALTH AND HUMAN SERVICES Public Health Service Alcohol‚ Drug Abuse‚ and Mental Health Administration National Institute on Drug
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melting point*Capillary tube *Tesco aspirin*Melting apparatus * synthesised aspirin | Apparatus for chromatography Materials for chromatography *Chromatography tank *Ethanol dichloromethane *Capillary tubes * Tesco pure aspirin *Weighing boat
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(Author’s name) (Professor’s name) (Course details) (Date Abstract The aim of the lab was to separate and analyse analgesic drugs in a drug tablet. The method used to separate the components was Thin Layer Chromatography (TLC) with silica adsorbent as the stationary phase and 0.5% glacial acetic as the mobile phase. In one plate‚ five known samples were used as the reference‚ that is: Aspirin; Caffeine; Ibuprofen; and Salicylamide. Aspirin and Salicylamide were the only samples that fluoresced
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than water) and will bond to the H+ ions. This resulted in a bright yellow mixture. Anhydrous CaSO4 is used as a drying agent. It absorbs all of the H2O in the mixture. If H2O is present‚ then the product cannot be analyzed by gas chromatography. Gas chromatography is used to determine the amount of
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