Search the web. Some interesting sites are listed below. Note that some of these sites go into much more depth than is reasonable for this course. http://en.wikipedia.org/wiki/Chromatography http://ull.chemistry.uakron.edu/analytical/Chromatography/ http://orgchem.colorado.edu/hndbksupport/TLC/TLC.html this is for TLC – similar to paper http://users.rcn.com/jkimball.ma.ultranet/BiologyPages/C/Chromatography_paper.html http://jchemed.chem.wisc.edu/JCESoft/Programs/CPL/Sample/modules/paprchrom/paprchromdesc
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Chromatography – Analyzing Analgesics by TLC and Isolation of β-Carotene by Column Chromatography Introduction/Background: Flavonoids are an important group of additives that can be defined as pure substances either natural‚ extracted from raw materials or synthetic. Chromatography is the separation of two or more compounds or ions caused by their molecular interactions with two phases – one moving and one stationary (Weldegirma 2012). Three types of chromatography are used
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Chromatography refers to a set of laboratory methods used in separating as well as purifying biomolecules. A variety of chromatography techniques exist‚ and all depend on the interaction between a stationary and a mobile state. Two types of chromatography methods were examined in this investigation. First‚ ion-exchange chromatography was used. This method separates ions and polar molecules based on their affinity to the ion exchanger [2]. Specifically‚ cation-exchange chromatography was performed
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Lab #3: Ion Exchange Chromatography Objective The purpose of this experiment was to separate proteins on the basis of their net charge at a particular pH. In cation exchange chromatography positively charged molecules are attracted to a negatively charged column. Conversely‚ in anion exchange chromatography‚ negatively charged molecules are attracted to a positively charged column. Experimental results could be monitored in a predictable way by controlling running pH‚ salt concentration‚ and by
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Introduction Paper chromatography is a method using a chromatography paper to identify the many mixtures of one simple color. The word chromatography comes from the Greek words‚ "chroma‚" meaning color‚ and "grafein‚" meaning to write. The process of this experiment is to place a colored dot‚ such as marker ink‚ onto the chromatography paper‚ one centimeter away from the triangular tip. Next dip your chromatography paper into the solvent‚ not allowing it to touch the colored dot. Let it sit for
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Paper chromatography is one of the easiest methods of chromatography. It is a method of planar chromatography (stationary phase is in form of a plane). Paper chromatography follows the basic principle of chromatography‚ which states that substances or components are distributed in between the stationary phase and the mobile phase. It is an analytical technique‚ where only a small amount of a sample is used for separating and identifying its components. Like any other method of chromatography‚ paper
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Solubility as a Property of Matter A Lab of Chemicals‚ Chromatography‚ and Crime! Chemistry is a natural science that deals with the composition of matter and the changes it undergoes. At crime scenes‚ investigators often find unknown materials that need to be identified. If an unknown material is a mixture‚ an investigator may want to know one or two things about it: What are the ingredients of the mixture? Is the mixture found at the scene the same as a known mixture? A mixture is a collection
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CHROMATOGRAPHY _TOPIC_ : 7.2 _RESEARCH QUESTION:_ What is chromatography? How many pigments are there in a plant? How the different pigments in a plant can be separated? _APPARATUS_ : Please refer to the handout _MATERIAL_ : Please refer to the handout _METHOD_ : Please refer to the handout _DATA COLLECTION_: Coloured band Pigment front distance (± 0.05cm) Solvent front distance (± 0.05cm) Green 11.7 13.0 Yellow 12.5 13.0 _DATA PROCESSING_: Coloured band Pigment
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provided for. Good luck!!! CHROMATOGRAPHY (By: Mayflor Markusic) KALIKASAN: Everyday Science in Action Volume 4 No. 1; pages 22-24 a) What is chromatography? 1 2 3 4 ____________________________________________________________ ________________ b) Give examples of mixtures which can be separated through chromatography. 4 5 6 7 ________________________________________ ______________________________________ c) How is chromatography done? 4 5 6 7 ________
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Paper chromatography is an important separation technique that depends upon differences in how strongly the dyes are adsorbed onto the paper (stationary phase) and how soluble the dyes are in the developing solvent (mobile phase). In paper chromatography‚ a small amount of the mixture to be separated is placed close to the edge of a piece of paper. The edge of the paper is then immersed in a developing solution. As the developing solution ascends up the paper by capillary action‚ the. components
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