GAS CHROMATOGRAPHY EXPERIMENT The purpose of this experiment is for the student: 1) to learn the general theoretical aspects of gas chromatography as a separation method‚ 2) to learn how to operate gas chromatographs specific to COD‚ 3) to become familiar with using the gas chromatograph (GC) to qualitatively identify components of mixtures‚ 4) to be introduced to and to interpret the quantitative data available via gas chromatography‚ 5) to gain insight into how the GC technique is used
Premium Chromatography Gas chromatography
of farUV: 180-240nm. 1. Near UV CD: 240n-320nm‚ Aromatic amino acids and disulphide bonds. 2. Visible CD: d-d transition in some metal protein complexes for eg Cu (II) prion. Principles of Chromatography Substances present in a mixture are allowed to distribute themselves between two phases: the stationary phase (fixed) and the mobile phase. As the mobile phase flows over the stationary phase‚ components of the mixture experience many transfers
Premium Chromatography High performance liquid chromatography
The Drosophila melanogaster In this experiment‚ the species Drosophila melanogaster‚ the common fruit fly‚ was studied to examine the processes of natural selection and genetic drift in the laboratory. The fruit fly has a sex linked gene determining eye colour. Located on the X chromosome‚ a fruit fly can exhibit the phenotype white eye (homozygous recessive)‚ or red eyed wild-type (dominant). Males of the species are XY‚ and as such only carry one eye colour determining X chromosome. Females
Premium Genetics Gene Biology
PAPER and COLUMN CHROMATOGRAPHY REPORT 1. 2. The unknown code is an amino acid‚ Glycine. To justify the reason is because the retention factor is the same exact number as the Glycine. The data of the unknown shows everything to be exact with the data of the Glycine 3. The mobile phase is the more polar during the capillary action of the experiment. As soon as the paper touches the mobile phase‚ the solvent rises to the amino acids. This is where you can find polarity of the amino acids.
Premium Amino acid
Analysis of Food Colorings by Paper Chromatography Introduction Paper Chromatography one method for testing the purity of compounds and identifying substances. This is a useful technique because it is relatively quick and requires small quantities of material. Separations in paper chromatography involve the same principles as those in thin layer chromatography. The substances are distributed between a stationary phase and a mobile phase. The stationary phase is usually
Premium Thin layer chromatography Chromatography Color
The fruit fly Drosophila melanogaster are an excellent specimen for research in genetics. Many reasons are that they have a rapid reproduction rate‚ easy to care compared other animals and less expensive. Researchers have determined the nucleotide sequence of nearly the entire 120 megabase euchromatic portion of the Drosophila genome (Cite). Drosophila has a simple genetic arrangement of only four chromosomes which contains three autosomes and one sex chromosome according to(--) and because of this
Premium Genetics Gene Biology
Genetics of Drosophila Lab Report: The test of heredity in fruit flies In my testings with breeding the fruit flies‚ I learned that the purpose of breeding them was to test how genetics pass from generation to generation‚ also known as heredity. We attempted to breed the flies and get them as close to a 9:3:3:1 ratio as possible. This would lead to an acceptable trait ratio. My hypothesis was that if we were to breed the flies in a stable environment‚ the most common fly (or the fly with the highest
Premium Male Female Sex
purpose of the experiment that has been conducted is to breed Drosophila melanogaster for specific traits. We used a dihybrid cross where the traits were sex linked. The cross is considered dihybrid due to the fact that we are crossing two different traits. The traits used for this dihybrid cross experiment are white eyes (w) and vestigial wings (vg). The white eyes are the sex linked trait. These traits were taken and crossed with wild type D. melanogaster. The experiment that we ran was
Premium
Chromatography is a process used to separate mixtures. The word chromatography is derived from the Greek words ‘khroma’ and ‘graphein’ meaning ‘color’ and ‘to write’ or to represent. Although there are a couple different types of chromatography‚ in each case a substance is placed onto or into a medium and a solvent is passed through the test substance. In chromatography science‚ the solvent is called the mobile phase or the carrier fluid and the medium is called the stationary phase. There are four
Free Chromatography
Candy chromatography is a method of analysis of separating and comparing of dyes used in candies and food coloring. It is also a proven method to analyze candy and its complex mixture‚ without having to taste it. These complex mixtures can be separated back into pure substances. To find out the ingredients of a favorite candy‚ chromatography would help to define every ingredient found in the candy sample. The word chromatography is derived from the two Greek words‚ "chroma" meaning color
Free Chromatography Thin layer chromatography