PROCEDURE Part I Setting Up Experiment 2D: Separation of a mixture by Paper Chromatography Objectives: 1. To assemble and operate a paper chromatography apparatus 2. To study and the significance of Rf values 3. To test various food colorings and to calculate their Rf values 4. To compare measured Rf values with standard Rf values 5. To separate mixtures of food coloring into their components 6. To identify the components of mixtures by means of their Rf values Materials / Apparatus: See “Health
Premium Color Primary color Food coloring
Brad Trent Biology 301L 22 April 2014 Drosophila melanogaster Lab Report Drosophila melanogaster Population Genetics Introduction Population genetics is a very important topic that has to deal with the structure of populations and how different factors and phenomena cause change in the populations genetic
Premium Genetics Evolution Biology
Experiment 9 Thin Layer Chromatography Experiment: 9 Title: Thin Layer Chromatography Introduction: Chemical analysis is conducted on specific analytes (specific substance of interest in a mixture)‚ however it is often found that these analytes must be separated for the chemical analysis to conduct their analysis. Chromatography is a set up of laboratory a technique that is used to separate a chemical mixture. This technique is very useful as it allows us to follow the course of
Premium Thin layer chromatography Chromatography
Chromatography of Commercial Analgesics Chromatography of Commercial Analgesics CHEMISTRY 200L EXPT 4 PAGE 8 - 11 CHEMISTRY 200L EXPT 4 PAGE 8 - 11 Janna Vernice R. Villalon*‚ Christian V. Villanueva‚ Cyd Vincent L. Zamora Department of Chemistry‚ College of Science *Corresponding author; e-mail: janna.villalon@rocketmail.com Abstract In analyzing the chromatography of analgesics‚ thin layer chromatography (TLC) was used. A very thin (micron) film of silica is coated on a glass
Free Chromatography Thin layer chromatography Chemistry
Austin Vandermey GS-1140 April 4th‚ 2015 Experiment We as a society have one big problem on our hands. This problem is sleep deprivation because there are not enough hours in the day to get stuff done. Between work‚ school‚ family‚ and leisure it seems like we do not have enough time to get are daily tasks done let alone have time for sleep. It is recommended by the U.S Department of Health that people need a solid eight hours of sleep to maintain good health. I don’t know about you
Premium Personal life Sleep Health
Chromatography Analysis of Black Pens to Determine Unknown Sample Purpose: Paper chromatography was performed on five different black pens‚ using four different solutions to determine which would be most appropriate to use on an unknown sample. Paper chromatography was then performed on the unknown sample to identify which pen was used to create it. Procedure: Each person participating in the analysis was assigned one solution to work with: V. Temple used distilled water‚ D. Sellers used
Premium Chemistry Experiment Analytical chemistry
Experiment #1: Introduction to Experimentation Submitted by: Neann Klara M. De Jesus BS Psychology II Submitted to: Dr. Geraldine E. Tria ABSTRACT SUMMARY The first experiment done by the class was called “Introduction to Experimentation”. Its main objective is to give basic knowledge about some of the logic of experimentation. The class was divided into groups of 2. In each group there was an experimenter (E) and a subject (S). The experimenter instructed
Premium Experiment Stanford prison experiment Theory
Problem How can you tell pigment separation by using Chromatography? Objective To prepare a chromatogram‚ separate pigments in a leaf and interpret the chromatogram. Hypothesis If I am to put a chromatography paper into a solvent‚ then it would separate the pigments depending on their Rf value. I think the pigments will separate in this order: Chlorophyll A‚ Chlorophyll B‚ Carotene‚ and Xanthophyll. Introduction Chlorophyll is the molecule that absorbs sunlight and uses its energy
Premium Chromatography Analytical chemistry Silica gel
Thin Layer Chromatography Introduction Thin Layer Chromatography or TLC is a technique used as a separation and identification technique. There are many forms of chromatography‚ but one thing that remains constant throughout all of the types of chromatography is that there is a stationary phase and a mobile phase. In the case of TLC the stationary phase is the silica gel on the TLC tray. Procedure Chromatograph method is a method of separating mixtures of two or more compounds. Two phases
Free Chromatography Thin layer chromatography
Title: Thin-Layer Chromatography Objectives: Part1: 1. To learn the technique of TLC and the visualization of colourless components. 2. To identify an unknown drug by a TLC comparison with standard compounds. Part 2: To learn the separation technique by using Thin Layer Chromatography plate in separating a mixture of compounds into individual pure compound by using Spinach Leaf. Introduction: (i) General Concepts Chromatography is a common and powerful method used to separate and analyze complex
Premium Chromatography Thin layer chromatography Analytical chemistry