A simple‚ specific‚ accurate and rapid reversed phase high performance liquid chromatographic method was developed for the simultaneous estimation of cilnidipine and chlorthalidone‚ using a Hiber® C18 (25cm x 4.6 mm)‚ Lichrospher® 100‚ RP-18 e (5µm) column and a mobile phase composed of phosphate buffer(pH 4.0 adjusted with orthophosphoric acid) : Acetonitrile (30:70‚ v/v). The retention times of Cilnidipine and Chlorthalidone were found to be 3.967 min and 6.427 min‚ respectively. Linearity was
Premium High performance liquid chromatography Chromatography Solubility
Gas chromatography is the common method used for the biogasoline analysis. Biogasoline catalytic cracking process requires detailed qualitative and quantitative analysis of the hydrocarbon composition of its oil and fraction. In this analysis‚ their fractional composition‚ saturated vapor pressure‚ density and antiknock rating will be investigated. By knowing the qualitative and quantitative composition of the gasolines‚ the content of n-paraffin‚ isoparaffin‚ aromatic‚ naphthene‚ and olefin (PIANO)
Premium Chromatography Gas chromatography Vapor pressure
References: Fessenden‚ J.‚ & Feist‚ P. (2001). Organic laboratory techniques. Australia: Brooks/Cole; pgs. 119-125‚ 133-138. Robards‚ K. (1994). Principles and practice of modern chromatographic methods. San Diego‚ California: Academic Press‚ Inc.; pgs. 1-34‚36-225. Williams‚ T. (1946). An Introduction to chromatography. London: Blackie & Son Limited; pgs. 22-34‚ 47-50. http://curlyarrow.blogspot.com/2011/11/lets-talk-about-tlcs-part-5-iodine
Free Chromatography Thin layer chromatography
.......................................... 409 16.2.3 Separation of Flavonoid Aglycones and Glycosides on Silica Gel and Reversed-Phase TLC....................................... 410 16.2.4 Use of Mathematical Methods for the Optimization of Chromatographic Conditions for Flavonoid Separations ........ 413 16.2.5 Multidimensional Planar Chromatography (Unidimensional Multiple Development and Two-Dimensional Development)..... 415 16.2.6 Quantitative Evaluation.....................................
Free Chromatography
is hard to distinguish the similar compounds because only one solvent is used unlike in 2-dimensional where in 2 solvents are used to separate the compounds. 3. What are the considerations in choosing a chromatographic solvent? - One of the considerations of a chromatographic solvent is whether it will bring out different Rf values of the samples used. If all of the compounds have the same Rf values then the separation of pigments would have failed due to the fact that all of them are
Free Chromatography Thin layer chromatography Analytical chemistry
Chapter II REVIEW OF RELATED LITERATURE AND STUDIES Review of Related Literature On mosquito larvae‚ the major larval habitats were anthropogenic‚ resulting from human behavior. According to the Journal of Vector Ecology (2012 p37) stated that “The management of insecticide resistance among malaria vectors needs urgent attention if insecticide-treated materials can continue to be used for malaria control.” Metarhizium anisopliae‚ a fungal pathogen of terrestrial arthropods‚ kills the aquatic larvae
Premium Mosquito
Results and Discussion – Expt. 3 (Crystallization) Name: Parts A and B: 1. Please comment on the relative purity of your recrystallized salicylic acid (Hint: You should use your empirical data to support your claim). -The Salicylic acid that I obtained had a melting point range around 157-158 degrees Celsius for Part A. For Part B the melting point range was 156-157.5 degree Celsius. Part B‚ I would say the sample is less pure because of the notion that impurities lower the melting point
Premium Temperature Purity Celsius
mixture. Example- analyte 1: analyte 2 = 1:2 2. preparative: for studying on a higher level There are three main components of which a chromatographic experiment consists of‚ these are the: 1. Mobile Phase: solvent into which sample is dissolved and helps sample travel through stationary phase. It is mobile. 2. Stationary Phase: Permanent part of chromatographic procedure‚ through which the mobile phase moves. It is stationary i.e. it doesn’t move. 3. Sample: This is the solution that has to
Premium Chromatography High performance liquid chromatography
and talked in a public speech to all people. She wants to change the habits of adults and children. Severn says‚ that she found fish with cancer. Is this the way we want to eat fish? We really have to think of what we can make better in waste seperation. So if for instance plastic is thrown in the sea‚ the fish will eat the plastic‚ and we eat the fish. Horrible! Another problem is the buying of unnecessary things. We buy and throw away. Why don´t we think of what we really need? In my opinion
Premium Rio de Janeiro Earth
High-performance liquid chromatography (formerly referred to as high-pressure liquid chromatography)‚ HPLC‚ is a chromatographic technique used to separate the components in a mixture‚ to identify each component‚ and to quantify each component. HPLC is considered an instrumental technique of analytical chemistry (as opposed to a gravimetric technique). In general‚ the method involves a liquid sample being passed over a solid adsorbent material packed into a column using a flow of liquid solvent.
Premium Chromatography High performance liquid chromatography Analytical chemistry