crystals started to melt in the fast ramp. The end temperature was 5°C above the end temperature in the fast ramp‚ and the ramp rate was 1°C /min. After running the slow ramp the melting point range was compared to possible known compound. The compound Fluorene was a possible match for what the unknown compound was. To confirm that the unknown compound was Flourene a mixed melting point was ran. In a weighing paper the unknown compound and flouerene was mixed and 1-2mm was placed in a capillary tube. A
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Experiment II. Separation Of a Sample Mixture By Liquid-Liquid Extraction Reading assignment: Techniques in Organic Chemistry 2nd ed pages 75-99. 3rd ed pages 113-140. Topics and Techniques i) identification of solvent layers of two immiscible solvents ii) partioning of a compound between two immiscible solvents and determination of KD iii) liquid-liquid extraction with aqueous acids and bases with organic solvents. iv) use of drying agents Introduction Liquid-liquid extraction is a method
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inhibiting enzymatic reactions involving para-aminobenzoic acid (PABA). PABA is needed in enzymatic reactions that produce folic acid which acts as a coenzyme in the synthesis of purine‚ pyrimidine and other amino acids. In part C‚ Fluorene was dissolved in three solvents. Fluorene(C13H10) is a polycyclic aromatic hydrocarbon. It forms white crystals that exhibit a characteristic‚ aromatic odor similar to that of naphthalene. It is combustible. It has a violet fluorescence‚ hence its name. For commercial
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protein and see the function of the protein. A protein can be analysed to study the structure of it and for ‘post-translational modifications’. This is when the protein is modified after the later stages of protein synthesis ‘translation’. Chromatographic method The purification of proteins requires several tehcniques and which technique to use is depedendant on what protein is being analayzed. The properties of the protein will be taken into consideration and what tecnhique suits best. The idea
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for H. sp. KHS3. To our knowledge‚ most of the studies on Halomonas sp. focused on degradation of monoaromatic rather than polycyclic and heterocyclic compounds. We have demonstrated that H. sp. KHS3 has the ability to growth using phenanthrene‚ fluorene and naphthalene as the only carbon source. Similar ability has been demonstrated by
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and 3423 cm-1. The C=O was found at 1681 cm-1 and the C-O was at 1280 cm-1. The IR for benzoic acid also displays its significant bonds‚ O-H and C=O. The O-H was between 2566 and 3222 cm-1 and the C=O stretch was found at 1685 cm-1. The IR for 9-fluorenone did not show any significant bonds except for at 1681 cm-1‚ the C=O stretch. To separate 4-aminobenzoate into an aqueous layer‚ HCl was added to protonate the NH2 side group and form a salt‚ creating its high solubility in water and low solubility
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scales. Identication (Thin Layer Chromatographic Identification Test for Crude Plant Drugs) – Test solution—Transfer 1.0g of dried allii sativi Bulbus Pulveratus to a suitable glass apparatus. Extract with 10mL of a mixture of methanol and water (95:5)‚ using any suitable method as described in Test Solution under Thin Layer Chromatographic Identification Test for Crude Plant Drugs. The filter is the Test Solution. Application Volume—5 µL Plate—Chromatographic silica gel. Developing solvent system—a
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the safe SEPERATION: Make regular bank run deposits throughout the day so large amounts of cash are not stored on premises. DUPLICATION: Not applicable – cannot duplicate cash Storage building – Our building could catch on fire due to the highly combustible type of storage materials. LOSS PREVENTION: Do not store highly combustible liquids inside of the building LOSS REDUCTION: Ensure adequate fire suppression equipment is located inside and is easily accessible SEPERATION: Divide the
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(separately!) combine your three samples of benzocaine‚ and combine your three samples of fluorenone. (If you misread this and mix everything together‚ no worries‚ you can just repeat the extraction to separate them again.) Now‚ one group is going to recrystallize the benzoic acid from water‚ one group is going to recrystallize the benzocaine from ethanol and water‚ and one group is going to recrystallize the fluorenone from
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foundations of this method were laid down at the end of 1960s and at the beginning of 1970s. The theory of chromatography has been used as the basis for System- Suitability tests‚ which are set of quantitative criteria that test the suitability of the chromatographic system to identify and quantify drug related samples by HPLC at any step of the pharmaceutical analysis. Retention Time (tR)‚ Capacity Factor k ’ and Relative Retention Time (RRT) The time elapsed between the injection of the sample components
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