Introduction: The purpose of this lab was to separate and purify a compound. This was achieved using techniques that allowed the extraction of the acid‚ the isolation of the neutral compound and the melting point classification of the neutral compound to test the purity of the sample. Chemical Reactions: HA + OH- A- + H2O R-COOH + OH- R-COO- + H2O Organic Acid Insoluble in H2O Conjugate base Soluble in H2O Procedure: A 0.170g sample consisting of a mixture of 0.110g
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Theoretical Ratio | Obs. Ratio | Obs.m.p. | Physical Appearance | Ethyl 4-aminobenzoate | .233g | .2093 | 89% | 30% | 20% | 89 ° C | Off white appearance; powdery | Benzoic Acid | .233g | .243 | 104% | 30% | 20% | 125 ° C | Clear crystals | 9-fluorenone | .311g | .623 | 200% | 40% | 60% | 82 ° C | Yellow‚ filmy‚flakes | Discussion: Based on the results‚ the most accurate extraction achieved was the first compound‚ ethyl 4-aminobenzoate. With an 89% actual yield of the compound‚ a 3rd or 4th
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Introduction Extraction involves dissolving a compound or compounds either from a solid into a solvent or from a solution into another solvent. Extraction is a method used to purify a substance by removing the impurities that was added to it. A solvent- solvent extraction is a type of extraction that is most commonly done by using two substances that are typically insoluble with each other. An acid-base extraction is a solvent-solvent extraction‚ in almost every case one of the solvents is water
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Date: Lab Section: Selected Known Compounds Name Structural Formula Literature Melting Point Acetanilide C8H9NO 114.3°C Fluorene C13H10 116.5°C Experimental Data Sample Composition 1. 100% Acetanilide 2. 10% Acetanilide 3. 25% Acetanilide 4. 50% Acetanilide Fluorene 5. 75% Acetanilide 6. 90% Acetanilide 7. 100% Fluorene Melting Range 108-116°C 108-112°C 105-112°C 102-109°C 105-112°C 109-115°C 108-114.5°C Graph of Data Discussion of Results The purpose
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of this experiment was to oxidize an alcohol (9-hydroxyfluorene) to a ketone (9-fluorenone) using aqueous sodium hypochlorite (bleach) as the oxidizing agent‚ while introducing techniques used in microscale experiments. Reaction: Results 1. Recrystallized Product Yield Product yield = (actual yield/theoretical yield) x 100% 3mL 9-hydroxyfluorene x (1mL/1000mL) x (0.09 mol/L) = 2.7 x 10-4 moles 0.05g 9-fluorenone / (180.20g/mol) = 2.77 x 10-4 moles Product yield: (0.000277 mol/0.00027 mol) x
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yielding a 95.8% recovery rate. As for the organic compound‚ 0.166g of fluorene was secured and its respective melting point range was 116.0 C – 117.0 C. The identity of final acidic product was revealed through the comparison in melting points of the three known products. The melting point range matched up similarly with 3-chlorobenzoic acid. The experiment began with 0.210g of the mixture of an unknown acid and fluorene. The yield in products
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made me think that we did something wrong and our results would not be accurate. Crystallization: Part B: Start Finish % Recovery Expected Remaining Sulfanilamide 0.093g 0.059g 68.6% 0.034g Part C: Start Finish % Recovery Expected Remaining Fluorene 0.044g 0.018g 48.6% 0.026g Melting Points: Compound Literature Melting
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inhibiting enzymatic reactions involving para-aminobenzoic acid (PABA). PABA is needed in enzymatic reactions that produce folic acid which acts as a coenzyme in the synthesis of purine‚ pyrimidine and other amino acids. In part C‚ Fluorene was dissolved in three solvents. Fluorene(C13H10) is a polycyclic aromatic hydrocarbon. It forms white crystals that exhibit a characteristic‚ aromatic odor similar to that of naphthalene. It is combustible. It has a violet fluorescence‚ hence its name. For commercial
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Experiment II. Separation Of a Sample Mixture By Liquid-Liquid Extraction Reading assignment: Techniques in Organic Chemistry 2nd ed pages 75-99. 3rd ed pages 113-140. Topics and Techniques i) identification of solvent layers of two immiscible solvents ii) partioning of a compound between two immiscible solvents and determination of KD iii) liquid-liquid extraction with aqueous acids and bases with organic solvents. iv) use of drying agents Introduction Liquid-liquid extraction is a method
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protein and see the function of the protein. A protein can be analysed to study the structure of it and for ‘post-translational modifications’. This is when the protein is modified after the later stages of protein synthesis ‘translation’. Chromatographic method The purification of proteins requires several tehcniques and which technique to use is depedendant on what protein is being analayzed. The properties of the protein will be taken into consideration and what tecnhique suits best. The idea
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