Determining the Effect of Light Intensity on Photosynthesis Avalon Pernell‚ et al Abstract: The purpose of this lab was to determine how light affected photosynthesis‚ specifically the production of O2 bubbles. It was predicted that when the light was more intense the O2 bubble production will be high. Conversely‚ when the light was less intense the O2 bubble production will be lower. Basically the plant that is closer to the light will produce more bubbles than the plant that is placed farther away
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2-15-11 Purpose: The purpose of this experiment was to take spinach leaves and extract the chlorophyll and carotenoid pigments by using acetone as the solvent. The chlorophyll and carotenoid pigments were extracted by using column chromography and alumina was used as the solvent. Solvents of different polarities were used‚ starting with the least polar‚ to extract the certain components from the leaves. They were then analyzed by using thin- layer chromatography. Procedure: The first part
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Experiment 3: Isolation of Chlorophyll from Spinach and Chromatography Performed February 14th and 16th By Kyle Herzog Organic Chemistry 344 Section 807 Spring 2012 Objective: The purpose of the experiment is to extract pigments from spinach leaves and separate them by column and thin layer chromatography‚ determining Rf values for the pigments. Equations: Rf= Distance travelled by a given compound/Distance travelled by a solvent solution 1. Crude- 4.25/5.5= 0.773 2. Carotene-
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behavior of two different genes for chlorophyll production in tobacco. Two sets of approximately 50-100 tobacco seeds were planted for one week. The plants were the F2 result of two sets of monohybrid F1 crosses Gg x Gg and CyCg x CyCg. A chromosome for these plants has two sets of genes. The gene at one locus has two alleles that code for normal chlorophyll production. Dominant allele represented as G produce the green phenotype. The mutant allele‚ g‚ produces no chlorophyll producing an albino phenotype
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Photosynthesis depends on an interaction between two sets of reactions: the light reactions and the Calvin cycle. Chlorophyll and the other molecules responsible for the light reactions are built into the thylakoid membranes. The enzymes that catalyze the Calvin cycle are located in the stroma. Beginning with the absorption of light by chlorophyll‚ the light reactions convert light energy into chemical energy in the form of ATP and NADPH. The ATP provides the energy‚ and the NADPH supplies the electrons
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Plant Pigment Lab Question: What pigments are present in Red Wandering Jews? Background: A leaf is filled with many pigments. The pigments are usually masked by the prevalence of the green chlorophyll. Anothocyanin(red or purple)‚ carotene(orange)‚ and xanthrophyll(yellow) are found in different proportions in different leaves. Leaf pigments can be separated by using paper chromatography. Paper chromatography is a technique that extracts pigments into a paper filter called chromatogram. What are
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fast green stock solution and chloroplast pigments‚ information about high absorbency wavelengths was gained. By plotting this information in an “absorption spectrum” graph‚ the maximum absorption was easily identified for fast green‚ chlorophyll A and chlorophyll B (refer to figures 1 and 4 within this lab). The graphs in general can be closely related and compared to Beer’s Law stated above. For example‚ when referring to figure 2 (within this lab) concentration curve for fast green‚ absorption
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From this experiment‚ I found that the ratios of chlorophyll: carotenoids: DNA: RNA: protein in Euglena cells were 1.000: 0.198: 4.254: 1.950: 37.017‚ respectively. The standard ratios of chlorophyll: carotenoids: DNA: RNA: protein are 1.00: 0.60: 5.00: 38.00: 446.00‚ respectively. By comparing the results‚ one will agree that the experimental order of abundance of each component
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photosynthetic eukaryotes (Biology 108 Lab Manual 2012). During eukaryotic evolution‚ red algae and green algae led to five supergroups of eukaryotes through a process of secondary endosymbiosis (Campbell et al.‚ 2008). Three basic photosynthetic pigments: chlorophylls‚ carotenoids‚ and phycobilins‚ each absorb light at different wavelengths due to different colors and different
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Introduction The purpose of this experiment is to determine the maximum absorbance of fast green‚ and the chlorophylls‚ also in the case of fast green create a concentration curve to determine an unknown substance. Each test will use the spectrophotometer. Spectroscopy is the study of light. A spectrophotometer is a machine used to determine the absorbance of light at any given wavelength. It does this by using a source of white light through a prism‚ which gives multiple wavelengths that
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