Protocol for Lab 5 – Aerobic Respiration Part 1 Isolation of Mitochondria from Cauliflower - Weigh 50g of rosettes cut from fresh cauliflower head. - Cut rosettes and place it on ice - Prepare juice extractor by placing ice and an empty 150 ml beaker into the right compartment. - Collect pulp from left compartment and record total volume of the extract. Approx. 20ml - Filter the pulp using six layered cheese cloth and collect it in a beaker sitting on ice. - Place two 50 ml test tubes
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ANAEROBIC AND AEROBIC METABOLISM Lab 5 BCEM 341 – Winter 2014 Nebojsa Kuljic 10066717 Partner: Kendra Skalyn B02 Introduction Cells of all organisms can obtain energy through the combustion of sugars‚ either in the presence of oxygen (Aerobically) or without oxygen (Anaerobically). The purpose of this experiment was to perform a quantitative investigation of the differences between Anaerobic and Aerobic metabolism using pea seedlings and yeast organisms [1].
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the mitochondrial membrane became "leaky" to hydrogen ions chemiosmosis stopped because there wasn ’t a gradient of protons between the outside and inside of the membrane. Without a gradient of protons the majority of the ATP produced by cellular respiration couldn ’t be produced which is essential to make fatty acids and breakdown glucose‚ therefore the energy in a persons metabolism comes from stored fat or protein‚ causing a person to lose weight as well as causing a lack of energy. In the same sense
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Conclusion I predicted that if the decolorization of DPIP is caused by photosynthesis and not cell respiration and spinach extract containing chloroplasts and mitochondria is incubated with DPIP‚ then the rate of DPIP decolorization should be higher if in bright light verses dark light because DPIP is reduced by photosynthesis and not by the mitochondria or any other cellular function. If DPIP was only decolorized by chloroplasts‚ then the percent transmittance of chloroplast suspensions would be
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know the elements of life – and how the reduction and oxidative of some of these drive bioenergetics oxidize carbon (i.e. glucose source) and reduce oxygen (electron acceptor) (CHNOPS) and iron (Fe; trace element; Fe also important to obtain cellular energy). Nutrition: CHNOPS Trace Elements: Iron (Fe)‚ Ni‚ Mg‚ Mo‚ Mn‚ Ca (important in humans due to osteoblastic activity)‚ Co Occupancy: beat out others for a spot to live‚ e.g. microbiota (normal flora) Resistance: defend against microbes‚ eukaryotes
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suggests a positive correlation. Plants require sunlight‚ water and carbon dioxide to grow. These are necessary in the process of photosynthesis‚ which makes glucose for the plants. The glucose is then used by the plant in the process of cellular respiration in order to make energy. 8. Conclusion:
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10.19.2011 TITLE: The Importance of Oxygen for Life PURPOSE: To determine whether test tubes filled with bacteria‚ a few salts‚ distilled water‚ and glucose would grow in the presence or absence of oxygen. By adding oxygen to certain tubes and withholding it from other tubes‚ one can determine which bacteria grow the most efficiently. HYPOTHESIS: The test tubes of bacteria with oxygen will grow and thrive over the test tubes without oxygen. METHODS: Using the data shown in Table 7-A-1
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Denisse Gayosso-Lucano BIO 1140 As industrialization and urbanization increased in America‚ plants‚ animals and humans became endangered by the rise in temperatures and carbon levels. It was then that Carla‚ a carbon atom was born at the Sahara desert. At night‚ Carla and her friends went outside to watch the stars peacefully. It had never occurred to them that the night would bring a sudden kidnap. All of Carla’s friends and Carla were attached to two oxygen atoms. Unfortunately‚ Carla fainted
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2. Describe the steps involved transforming energy from burning glucose to synthesis of ATP. Cellular respiration‚ in the process of ATP synthesis‚ refers collectively to intercellular reactions in which energy-rich molecules are broken down to form ATP‚ using O2 and producing CO2 (2). In most cells‚ ATP is generated from the disassembling of absorbed nutrient molecules in three stages‚ glycolysis: in the cytosol‚ the citric acid cycle: in the mitochondrial matrix and oxidative phosphorylation: at
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Respiration: How Cells Harvest Chemical Energy 1. A cell must work just to maintain its complex structure‚ for order is intrinsically unstable. Because of entropy (the second law of thermodynamics)‚ which states that any enclosed system tends toward disorganization‚ cells must constantly use energy to keep themselves in order. If the cell was to stop using energy and let itself go then it would eventually get so disorganized that it wouldn’t function properly. 2. With the help of enzymes‚ the
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