CELL TRANSPORT MECHANISMS AND PERMEABILITY Zack Johnson Human Anatomy & Physiology Lab 2101 Instructor: Alan Byboth‚ M.S. 10/4/12 Abstract- The cell is invaluable building block of all biological life on this planet‚ and one of its most important and unique characteristics is its ability to be selectively permeable with its plasma membrane. This outer membrane’s sophisticated mechanisms of transport through its bilayer are vital in maintaining homeostasis in the cell and the entire
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Lab 1: Cell Transport Mechanisms and Permeability Purpose The purpose of this experiment is to have a better understanding of the subject matter and to understand the difference between active and passive cellular transport. It will also help to define diffusion and describe the process that occurs when certain solutes come in contact and are either stopped or move through the plasma membrane. Hypothesis I believe the plasma membrane is selective about what is able to and not able to pass
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Experiment 2 The Cell Membrane Abstract The boundary between any cell and its environment is the plasma membrane‚ composed of a matrix of phospholipid molecules along with a number of different kinds of proteins. Membranes have different properties and a variety of functions‚ in large part determined by the specific proteins within the membrane. This experiment is designed to determine the stress that various factors‚ such as osmotic balance‚ detergents and pH‚ have on biological membranes. There are three
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BioLab3 Cell Membranes Lab Report Student Name: I. Diffusion Define the following terms. Solvent Solute Solution. Diffusion Concentration gradient Dynamic equilibrium EXERCISE 1 – Factors influencing rate of diffusion Predict how molecule size and temperature will affect the outcome of this experiment. Record the data from the information in the lab. Potassium Permanganate R.T. Methylene Blue R.T. Time (min) Total Diameter
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twentieth century‚ little was known about cell membranes. Until the early 1950s‚ the biological cell membrane was rarely mentioned in scientific literature. It was recognised that something was probably there‚ but hardly anything about it was known. Considering the lack of technical equipment available a century ago‚ scientists such as Charles Overton and Edwin Gorter were not only exploring new territory in looking at the properties of cell membranes‚ but laying the way for future cell biologists
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PGEG381 L50 Rock Mechanics & Reservoir Laboratory Spring 2010 Lab Session No.5 Absolute Permeability Using a Gas Table of Contents 1.0 Abstract ……………………………………………2 2.0 Equipments used ………………………………………2-3 3.0 Test Procedures ……………………………………...3-5 4.0 Results ………………………………………………….5-7 5.0
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3.1 BIOLOGICAL MEMBRANES 3.1.1 Properties of Cell Membranes • Separates living cell from its nonliving surroundings. • 8 nm thick. • Selectively permeable - allows some substances to cross more easily than others. 4.1.2 Fluid Mosaic Model • Singer and Nicolson (1972) - plasma membrane is a mosaic of proteins dispersed within lipid bilayer‚ with only bilayer‚ the hydrophilic regions exposed to water. Hydrophilic region of protein Phospholipid bilayer Hydrophobic region of protein
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PERMIABILITY TEST AIM OF THE EXPERIMENT: To determine the coefficient of permeability of a given soil sample by Variable head permeability test. APPARATUS REQUIRED: a) Special: i. Jodhpur permeameter frame consisting of sand pipe graduated scale‚ rubber tubing connected to permeameter mould. Permeameter mould. Accessories of permeameter mould including the cover‚ base‚ detachable collar‚ porous stones‚ dummy plate etc. Round filter paper. Dynamic compaction device. ii. iii. iv. v. b) General:
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CHEMICAL MEDIATORS OF INFLAMMATION Cell derived mediators Mediator | Source | Function | Vasoactive amines | Histamine | Mast cells‚ basophil‚ platelet | Vasodilation‚ ↑ vascular permeability‚ endothelial activation | Serotonin | platelets | Vasodilation‚ ↑ vascular permeability | Eicosanoids | Prostaglandins | Mast cells‚ leukocytes | Vasodilation (PGD2‚PGE1‚E2 & PGF2-α)‚ pain (PGE2)‚ fever | Thromboxane A2 | | Promotes platelet aggregation‚ vasoconstriction | Prostacyclin
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MOVEMENT ACROSS MEMBRANES Intracellular fluid Extracellular fluid Pond water Blood plasma K+ A- ClNa+ Cl+ K+ Na+ Cl- Na+ Na+ Gill epithelial cell Intestinal epithelial cells Cl- Cell membrane Anionic proteins (a) Ion concentration inside a single animal cell (b) Ion concentration across gill epithelium of a freshwater fish Blood capillary Intestinal lumen Glucose from meal Cross section of small intestine (c) Glucose transport across intestinal epithelium into
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