testing the effect of temperature on the plasma membrane of a beetroot. I will do this by measuring the percentage light transmission using a spectrophotometer. Once I have obtained my results‚ I will justify them using scientific knowledge explaining what I have observed whilst carrying out the experiment. Factors to be controlled "« The pH concentration "« Regular use of distilled water "« Ensuring that the surface area of each piece of beetroot is the same "« Use the same volume of water "«
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Investigating the Effect of Temperature on the Permeability of Membranes The permeability of membranes can be altered in several ways. From previous preliminary work I know that reagents‚ for example detergents and alcohol alter the arrangement of the phospholipids in the membrane allowing substances to leak out of the cell. Temperature also affects the membrane. As membranes relay on protein molecules to allow molecules to enter and leave the cell through facilitated diffusion the temperature must
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To investigate the effect of variation in temperature on the permeability of cell membranes using fresh beetroot Aims In this investigation‚ you will subject fresh‚ washed beetroot discs to different temperatures to investigate the effect of temperature on the permeability of the plasma membrane. Overview The beetroot discs will be placed in distilled water at different temperatures. You will compare the intensity of the beetroot pigment that has leaked out from the cells subjectively and possibly
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The effect of temperature on the cell membranes of beetroot cells and amount of pigment released. Apparatus • Corer size 4 • White tile • A Beetroot • Automatic Water Bath • Segregated knife • A thermometer • Stopwatch Method: • First take the white tile and the corer. Then collect a cylinder of beetroot by pushing the corer into the beetroot and withdrawing it. The cylinder remains inside the corer- so push it out with the end of a pencil. • Collect 3 cylinders
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Effects of Temperature on Beetroot Cell Membranes Background Information: A cell membranes is a thin structure that surrounds the whole cell. It contains the cytoplasm of a cell. The cell membrane is made up of hydrophilic region and a hydrophobic region. The hydrophilic region likes water‚ it is on the outside of the cell‚ the hydrophobic region is the inside of the cell where its protected from H2O. The cell membrane’s outer surface lets larger molecules into the cell. The inner surface deals
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the effect of the organic solvents on the cell membrane of the beetroot Objective: To investigate the effect of different organic solvents‚ such as alcohol and paraffin oil‚ on the cell membrane of the beetroot by using the red pigments in the beetroot as indicator. Hypothesis: Organic solvents dissolve the organic matter in the cell membrane (such as phospholipids). This destroys the cell membrane‚ and the permeability of the cell membrane is disrupted casing red pigments to leak out by
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temperatures will affect the cell membrane‚ thus would then releases the purple pigments out of the vacuole which causes the leakage of the purplish liquid. Background Information The outermost layer is the cell wall‚ which is present only in plant cells and is made up of a carbohydrate called cellulose and also has other protein substances embedded within it. The cell wall is a rigid layer and gives structural stability to the cell and also limits the permeability of large substances into and out
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Purpose: Examine the role of the cell membrane in the cell by disrupting its function using temperature (Biology 107 Laboratory Manual 2014). This will improve the general understanding of optimal growing temperatures and the breakdown of the cell membrane Procedure: Betacyanin solution of a known concentration was diluted to create a dilution series‚ then placed in a spectrophotometer set to 525 nm. The absorbance of the dilution was used to create a standard curve for betacyanin. Discs of living
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SITI ALIA IZZANI ISMAIL M13D Name : SITI ALIA IZZANI BINTI ISMAIL Class : M13D Date : 6th September 2013 Title : Movement through the plasma membrane Hour : 2 hours Assessment : Design Aim : To study the effect of lipid solubility of molecules on permeability of plasma membrane of animal cell. Introduction : Diffusion: In general‚ diffusion process occur when there is a concentration gradient between two region .Molecules (other than water) will move
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Beetr------------------------------------------------- The Effect of Temperature on Membrane Permeability in Beetroot Cells Stage 2 Biology 2013 Results Figure 1 Temperature | Absorbance at 530nm | 30°C | 0.407nm | 40°C | 0.457nm | 50°C | 0.518nm | 60°C | 0.622nm | 70°C | 0.672nm | 80°C | 0.859nm | The table above gives an example
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