ACTIVITY 1 Simulating Dialysis (Simple Diffusion) 1. Describe two variables that affect the rate of diffusion. __The size and the concentration. ___ 2. Why do you think the urea was not able to diffuse through the 20 MWCO membrane? How well did the results compare with your prediction? __ The molecules were to large to go through. My results matched._ 3. Describe the results of the attempts to diffuse glucose and albumin through the 200 MWCO membrane. How well did the results compare with your
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TITLE AND AUTHOR Lab 7 Analysis of purified Concanavalin A via:Hemagglutination INTRODUCTION The purpose of this lab was to test the biological activity of ConA by performing a hemagglutination assay. If ConA is active then agglutination will occur due to ConA’s free receptors being able to bind to the glucose residues on the sheep’s red blood cells. If ConA is not active then no agglutination will occur. To test the hemagglutination reaction‚ two types of ConA solutions
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01) The Cell Structure and function Abstract In this lab the structures of various cells were viewed through a compound microscope. The bacteria in yogurt was viewed a long with a slice of potato‚ and a thin skin of red onion. The potato was dyed with iodine while the red onion was dyed with methane blue. The students had to assemble the first three slides. Two more slides were provided these are the yeast and protozoa cells. Those two are also viewed
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BioLab3 Cell Membranes Lab Report Student Name: I. Diffusion Define the following terms. Solvent Solute Solution. Diffusion Concentration gradient Dynamic equilibrium EXERCISE 1 – Factors influencing rate of diffusion Predict how molecule size and temperature will affect the outcome of this experiment. Record the data from the information in the lab. Potassium Permanganate R.T. Methylene Blue R.T. Time (min) Total Diameter
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Under Microscope Lab Report A. The purpose of the investigation is to determine the time an onion cell spends in each part of the cell cycle. B. The significance of this investigation is to help us understand and know how long cells are undergoing mitosis. My personal interest is to know the phases of mitosis in cells in order to someday help find cures for cancer. C. The cell cycle is a chronological set of events‚ culminating in cell growth and division into two daughter cells. Some of the reasons
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Mammalian Cell Culture Lab Manual Student guide of Cell Biology Laboratory Techniques 2012 Jay M. Feldman Cell Biology Professor Lagier 11/26/2012 Mammalian Cell Culture Lab Manual Student guide of Cell Biology Laboratory Techniques 2012 Jay M. Feldman Cell Biology Professor Lagier 11/26/2012 Table of Contents I. Introduction * About the author page 2 * Preface page 3 II. Laboratory Techniques * Cell Culture Technique page 4-5 * Sterile Technique
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Title: The effect on temperature on beet cell membranes. Introduction A cell membrane or plasma membrane is very important in protecting cells. A cell membrane is a thin layer that protects the cells. The membrane is filled with lipids and proteins. The cell membrane protects the cell from ions and molecules that enter and exit the cells. I hypothesize that at -5 degrees Celsius‚ the color intensity will be a 1. I hypothesize that at 5 degrees Celsius‚ the color intensity will be a 2
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John Leone Lab Partner(s) Ethan Davis & Ryan Hanna Date: 12/10/14 Instructor: Dr. Chuckran Period: H Plants and Animal Cells Abstract: In this lab‚ you will find out how plant and animal cells are alike and how they are different. The onion elodea cells were both plants‚ so they had a cell wall. The cheek cells were animal cells‚ so they did not have a cell wall. Eukaryotic cells contain structures called organelles that carry out life processes. Eukaryotic cells can be classified by the types of organelles they contain
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and centrifuged for 20 seconds. A polyacrylamide gel was assembled and the samples were loaded into it. The gel ran for approximately 45 minutes at 160 V. A western blot analysis was done in order to examine the Erk1/2 phosphorylation of the NIH3T3 cells. Three pieces of Whatman paper were wet in transfer buffer and placed on the anode plate. A wet PVDF membrane was placed on top of the Whatman paper. The gel was removed from holder and the stacking gel was removed. Forceps were used to place the gel
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Methods for studying cells in the lab Centrifugation A centrifuge is a device for separating particles from a solution according to their size‚ shape‚ density‚ viscosity of the medium and rotor speed. Centrifugation can be used to separate cells from one culture or to isolate an organelle from the rest of the cell. The process relies on speed; the faster and longer the particle is centrifuged‚ the smaller the particles are that will be separated. Separation occurs by causing the larger particles
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