Cell Fractionation * Cell Fractionation: takes cells apart and separates the major organelles and other subcellular structures from one another. * Tissue cells are the first one to be homogenate or broken apart. * Plasma membranes are broken up so that there internal contents spill out and mix together and this is called homogenate. * Homogenate is in spun in a higher rate of speed in a process called centrifugation. And that speed can vary that why it’s called differential
Premium Cell Organelle Bacteria
Cell Fractionation: Isolation of Mitochondria from Cauliflower and Determination of Specific Enzyme Kinetics Introduction Mitochondria is an organelle found in eukaryotic cells that play a role in biochemical processes such as respiration and energy production. Mitochondria even play an important role in apoptosis‚ or programmed cell death. This is achieved by disruption of electron transport‚ oxidative phosphorylation‚ and ATP production or even the release of proteins that trigger activation
Premium Citric acid cycle Cellular respiration
Cell Fractionation i) EQUIPMENT: Cold Liver‚ Blender‚ Gauze‚ Fridge‚ Isotonic Solution‚ Centrifuge‚ Tubes‚ Scalpel‚ HOMOGENIZATION • Take chilled liver tissue and cut into smaller pieces. • Place liver into blender with cold isotonic solution. • Blend until you have a thick soup like substance (homogenate). • Filter the mixture to remove large debris through gauze into a tube. DIFFERENTIAL CENTRIFUGATION • Attach tubes of homogenate to the refrigerated Centrifuge and turn on to spin
Premium Temperature Celsius Mitochondrion
Result: Part A: Table 1: Fractionation of amino acid Fraction No | Response to Ninhydrin test(level of blue colour change) | Response to Sakaguchi test (colour change) | 1 | X | yellow | 2 | Dark blue | yellow | 3 | Dark blue | Pale orange | 4 | Blue | Pale orange | 5 | Pale blue | Pale orange | 6 | Blue | Pale orange | 7 | Pale blue | Pale orange | Discussion Part A In the separation and purification of a single protein or amino acids‚ a solution containing the desired
Premium Amino acid Protein Acid
Cell Transportation Lab Eggs are a great example of a cell. Underneath the hard shell is a thin membrane that is just like the cell membranes in your cells. There are microscopic pores in the membrane that allow substances to move in and out of the cell. Way Cool! Recall that the function of the cell membrane is to control the internal balance of the cell. It helps to maintain homeostasis. In this lab you will use vinegar‚ an acid‚ to dissolve the shell off of the egg to expose the membrane
Premium Cell membrane Membrane biology Cell wall
Introduction Using microscopes allows humans to see things they’ve otherwise would have never seen before‚ like cells. A cell is the basic unit of life. All living things are made of cells. All cells come from preexisting cells through a process called cellular division. There are two types of cells‚ eukaryotes and prokaryotes. Prokaryotes are very simple and small. They are unicellular and have no membrane bound organelles. Their DNA is found directly in the cytoplasm since they have no nucleus
Premium Cell Organelle Eukaryote
Lab #1: Cell Membrane Prepared for Gary V. Lawrence Biology 0983 By Zane Jeffels Partners: Lily Juno‚ Huynh‚ and Lin Yan Sun Preformed: October 11th‚ 2010 Due: October 25th‚ 2010 King Edward Campus- Rm. 3275 Vancouver Community College Abstract The purpose of this lab was to determine if hemolysis would occur‚ and how long it would take to occur to red blood cells when blood suspension is introduced to solutions prepared at different temperatures
Premium Red blood cell Cell membrane Protein
Cell Biology Lab - Homework 1 Due to the week of Oct. 6-10th This is NOT a lab group project. Do the work individually. You may use any source other than your fellow students. Show your work‚ use your own words and cite all references. 1. (20 points) The HeLa S3 cancer cell line is considered to be “continuous” due to acquired mutations that enable the cells to evade senescence. This property is one of several hallmark characteristics of cancer cell lines. Describe three hallmark characteristics
Premium Cell culture
The Onion Cell Lab Background: Onion tissue provides excellent cells to study under the microscope. The main cell structures are easy to see when viewed with the microscope at medium power. For example‚ you will observe a large circular nucleus in each cell‚ which contains the genetic material for the cell. In each nucleus‚ are round bodies called nucleoli. The nucleolus is an organelle‚ which synthesizes small bodies called ribosomes. Ribosomes are so small you cannot see them with the light microscope
Premium Cell nucleus Bacteria Cell
STRUCTURE AND FUNCTION OF LIVING CELLS Name Answer the following questions as you work your way through the lab material typing in your answers. Then submit your completed lab report through the “Assignment” feature. This lab report is worth 50 points towards your final lab grade. Also‚ per the Honor Code‚ this work must be your own. CELLS Using your Biology Textbook answer the following questions. 1. What types of organisms are Prokaryotes? The Prokaryotes are organisms that are
Premium Brain Nervous system Neuron