The control group of this experiment was room temperature. The constant variables were‚ the amount of distalled water‚ catechol substrate‚ and potato extract in each tube was the same. (2ml) The size of the test tubes were the same also.
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large pieces in the solution. The solution created was catechol. Five different solutions were prepared as blanks with each test tube containing 6.0mL of a different pH (pH 4‚ pH6‚ pH7‚ pH8‚ pH10) of phosphate buffer‚ 1.0mL of the enzyme and 1.0mL of water. Five more solutions were prepared in the same fashion except without the 1.0mL of water. These five experimental solutions were capped and mixed by inversion. After mixing‚ 1.0mL of catechol was added to each of the five experimental solutions and
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NAME: Aleema Chelsea Chinchamee LAB PARTNERS: Karishma Ramrattan‚ Vishma Ramsumair and Sharona Badree ID #: 814003081 DATE (of lab session): Tuesday 24th March‚ 2015 DEMONSTRATOR: Maurissa Course Code & Title: BIOL 1362- Biochemistry I Title of Lab: Investigating Enzymatic Activity in Sweet Potato‚ Irish Potato Extract and Milk. Aims: 1. To determine the effect of ascorbic acid on Polyphenol Oxidase (Phenolase). 2. To determine the level of specificity of Phenolase using the
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water‚ 6mL of Catechol‚ 1.5mL of Extract(potato)‚ a Spectronic 20 (spectrophotometer)‚ Wax pencil‚ a Test tube rack‚ three Parafilm‚ tissues to clean the Spectronic 20 and the test tubes‚ 3 pipets and a timer. So we won’t confuse ourselves‚ we used the wax pencil to label each test tube as we placed the required amount of substance in each of them. To avoid any slight errors‚ we also used three different pipets for the three required materials‚ which are the distilled water‚ catechol and the extract
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Epinephrine Structural Diagram- page 72 [pic] About: - Epinephrine has several uses‚ including increasing ventilation of lungs through relief of bronchodilation‚ increasing cardiac output‚ increasing systolic blood pressure‚ dilating blood vessels involved in crisis response - Clinically‚ epinephrine plays a lifesaving role in countering the effects of anaphylactic shock. It relaxes smooth muscle‚ though at different receptors. Physical Properties: - high boiling points and melting
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Benzene 1 Benzene Benzene Identifiers [1] CAS number 71-43-2 PubChem 241 ChemSpider 236 UNII J64922108F EC number 200-753-7 KEGG C01407 ChEBI CHEBI:16716 ChEMBL CHEMBL277500 RTECS number CY1400000 Jmol-3D images Image 1 [2] [3] [4] [5] [6] [7] [8] [9] Properties Molecular formula CH Molar mass 78.11 g mol−1 Appearance Colorless liquid Odor aromatic‚ gasoline-like
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Cyclic voltammograms for 50 moldm-3 catechol were recorded with plain GCE‚ PEDOT-GO‚ PEDOT-rGO and PEDOT-rGO incorporated with LAC at pH 7.0 and scan rate 50 mVs-1 Fig. 4.3.4. Fig 4.3.4A present CVs and the anodic peak current observes for dopamine are bare small redox peak was observed. The modification of PEDOT-GO and PEDOT-rGO were increase in increase in the redox reak compared with bare electrode. The incorporation of LAC on the PEDOT-rGO gives decrease in the oxidation and increase in the
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“CLONING OF HORSE RADISHPEROXIDASE GENE IN E.coli FOR EXTRACELLULAR EXPRESSION” Project report submitted to Jawaharlal Nehru Technological University Hyderabad in partial fulfillment for the award of degree of Bachelor of Technology in Biotechnology Submitted by M.RAVI TEJA (08311A2338) & V.MAYURI (08311A2358) Under the Guidance of DR.CHAND PASHA Department of Microbiology Osmania University [pic] Department Of Biotechnology SREENIDHI INSTITUTE OF SCIENCE AND TECHNOLOGY Yamnampet
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Question 1 1. How is the neurotransmitter acetylcholine inactivated at the parasympathetic terminal receptor site? Answer A. The the enzyme catechol O-methtransferase (COMT) B. By the reuptake process C. By the enzyme cholinesterase D. By the enzyme monoamine oxidase E. By the enzyme nicotinase 1 points Question 2 1. You adminster an inhaled bronchodilator that is known to have adrenergic side effects on the heart. What clnical sign should you watch for in
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dopa decarboxylase. Breakdown: Dopamine can be broken down by either reuptake or metabolism. With metabolism dopamine breaks down to 3‚4 DOPAC by monoamine oxidase or dopamine is broken down to 3-MT by catechol 0-methyltransferase. The3‚4 DOPAC than breaks down to homovanillic acid by catechol-o-methyltransferase and 3-MT breaks down to homovanillic acid by monoamine oxidase‚ aldehyde dehydrogenase. (e) Briefly outline one mechanism for the possible genetic basis of alcoholism. GABA is a neurotransmitter
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