An Investigation on the rate of reaction of the enzyme Catalase on the substrate Hydrogen peroxide. Plan Aim: To investigate the rate of the effect of Catalase on hydrogen peroxide. Introduction This investigation will be carried out to investigate the rate of reaction of the enzyme catalase on the substrate hydrogen peroxide. Enzymes are biological catalysts‚ which speed up the rate of reaction without being used up during the reaction‚ which take place in living organisms. They do this by
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THE EFFECT OF CATALASE ON HYDROGEN PEROXIDE The aim of the experiments is to see if increasing the surface area of the enzyme Catalase‚ affects the relative activity of the substrate Hydrogen peroxide. Then to observe and measure the effect the Catalase has on the chemical breakdown of the hydrogen peroxide. My theory is if you keep increasing the surface area of Catalase‚ the more active sites are available to join with the substrate causing an increase in the breakdown of the hydrogen peroxide
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Biochemical Engineering James M. Lee Department of Chemical Engineering Washington State University Pullman‚ WA 99164-2714 jmlee@wsu.edu Chapter 1. Introduction ................................................................ 1 1.1. 1.2. 1.3. 1.4. 1.5. 1.6. Biotechnology .............................................................................. 1 Biochemical Engineering............................................................. 2 Biological Process....................................
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Enzymes Enzymes are the sparks that start the essential chemical reactions our bodies need to live. They are necessary for digesting food‚ for stimulating the brain‚ for providing cellular energy‚ and for repairing all tissues‚ organs‚ and cells. There are three types of enzymes: metabolic enzymes‚ digestive enzymes‚ and food enzymes. Metabolic enzymes catalyse‚ or spark‚ the reactions within the cells. The body’s organs‚ tissues and cells are run by metabolic enzymes. Without them our bodies
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Enzymes Enzymes are… * Biological catalysts Lower the energy level needed for a biochemical reaction to occur. This energy level is called activation energy. * Proteins Polypeptide chains made up of 100’s-1000’s of amino acids in a specific sequence. * Do not get “used up” in a reaction The number of “uses” of an enzyme depends on the enzyme. * Work more efficiently at certain optimum temperatures. * They are “reaction-specific”. Each enzyme is included in one reaction.
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Enzymes are biological catalysts or assistants that consist of various types of proteins that work to drive the chemical reaction required for a specific action or nutrient. They can either launch a reaction or speed it up. Catalase is a common enzyme found in nearly all living organisms exposed to oxygen. It is a very important enzyme in protecting the cell from oxidative damage by reactive oxygen species (ROS). The catalase used in this experiment will come from five different sources: Spinacia
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Based on the figure above‚ it is clearly showed that the trend line was gradually increased before it dropped after achieved at one maximum point. This maximum point‚ pH 6 was the optimum pH for the turbidity removal for this water sample. Note that the final pH of treated water were dropped slightly from its initial pH. For the optimum pH‚ the pH was dropped from pH 6.50 to 4.11 which caused the water to become acidic. The pH were dropped because when the alum dissolved in water‚ aluminium‚ sulphate
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Root mean square deviation (RMSD): The root mean square deviation (RMSD) is a measure of structural differences that are observed on superimposition of two protein structures at a different time points i.e. current position and previous position. The RMSD values for backbone atoms of each residue were calculated for entire trajectory to see the evolution of structure with time. The RMSD plot is shown in Supplementary Figure S7 where timestep is plotted on X-axis while RMSD (Å) is plotted on Y-axis
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Structure: Enzymes are globular proteins that act as catalysts‚ they have a specific 3D shape that is the result of their amino acid sequence. There is a specific region of the enzyme that is functional‚ this is called the active site. The active site is made up of a small number of amino acids and forms a small depression within the larger enzyme molecule. Moreover‚ the molecule that the enzyme acts upon (substrate) fits precisely into the depression and forms an enzyme-substrate complex. The substrate
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Potato Enzyme Lab Enzymes are protein catalysts that speed up chemical reaction in living organisms. The purpose of this lab was to; observe the breakdown of hydrogen peroxide toxin by potato’s enzyme catalase‚ determine factors that influence how quickly the reaction takes place‚ and determine factors that influence how well enzymes function. The first test‚ we were supposed to see what would happen when we add sand in hydrogen peroxide. We filled a test tube with 5mL hydrogen peroxide and we added
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