from boiling water) •4 coffee cups •cheesecloth to filter the potato extract •a wax pencil or Sharpie marker •a ruler •scale or balance •250 ml graduated cylinder •oven mitt or tongs Preparation Step 1: Research (online) the reaction between catalase and hydrogen peroxide and be familiar with the reactants‚ products‚ and enzyme. You will include the overall reaction in your lab report for this experiment. We will measure enzyme activity by measuring the height of the bubbles produced. Questions
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designed to investigate the effects of temperature on reactions between the enzyme catalase found in animal tissue with the substrate H2O2. The hypothesis stated that an increase in the temperature of the substrate would create a subsequent increase in the rate of reaction between the enzyme and the substrate. This hypothesis was tested by immersing 1cm cubes of animal tissue (sheep liver) which contained the enzyme catalase into the substrate (H2O2 ) mixed with detergent which foamed showing a visual
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OBJECTIVE: 1. To distinguish the bacteria abilities to metabolize various substrates and end products formed. 2. To observe the growth of different bacteria species in term of structures and its morphology based on different chemical substance applied. 3. To observe physiological and immunological properties utilized by different species of bacteria. INTRODUCTION: Bacteria biochemical testing can determine the types and numbers in terms of colony forming units of bacteria present in a
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Purpose: Cells produce toxic wastes‚ in this experiment hydrogen peroxide‚ and without some sort of molecule to break it down the cell will die‚ along with the organism itself. However with the aid of an enzyme‚ catalase‚ hydrogen peroxide is able to be broken down into an intermediate and thereafter harmless substances water and oxygen. The goal of this lab is to measure the reaction rate of this process in different substances such as a liver‚ a vegetable‚ and breast tissue. By using variables
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Part B: Practical Report The Effect of Temperature on Enzyme Activity Aim: To investigate how temperature effects the enzyme catalase. Hypothesis: If the temperature of water is increased then the enzyme will react quicker to form oxygen and water‚ when compared to cold water. Purpose: To design and conduct a plan of a practical about the effects of temperature on enzymatic activity with a partner. Introduction: An enzyme is a protein‚ which speeds up a specific chemical reaction without altering
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TEKNOLOGI MARA BIO 411 CELL BIOLOGY PRACTICE 3 REDOX -REACTION/CATALASE ACTIVITY GROUP MEMBERS: Syazani Zahier Bin Zakaria (2014292204) Nur Fatin Syamimi Binti Ahmad Tarmize (2014236664) Zuliakha Binti Zolkafali(2014279698) Nur Izzati Binti Azizan(2014284578) GROUP:ASB1Cb DATE OF SUBMISSION :12 November 2014 INSTRUCTOR: Miss Ellia Kartini Mujar OBJECTIVES 1. To demonstrate reduction-oxidation reaction on living tissue. 2. To compare enzyme catalase on plant and animal tissues. 3. To measure the rate on
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which catalase decomposes H2O2. After adding H2SO4 for different time lashes‚ etc.‚ the resulting data will be graphed at which the catalase decomposed by catalase. Background: The four different activities to the enzyme catalyst lab have similar but different backgrounds. Activity A’s background is to investigate the specific reaction of the decomposition of hydrogen peroxide by the enzyme‚ catalase. Hydrogen Peroxide decomposes slowly into water and oxygen‚ and the addition of catalase lowers
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reaction rate of liver catalase. Hypothesis: Null Hypothesis: If the concentration of the Hydrogen Peroxide is changed then there would be no change in the reaction rate. Alternate Hypothesis 1: I there is an increase in concentration in concentration of Hydrogen Peroxide then the reaction rate of the liver catalase will increase. Alternate Hypothesis 2: If there is an increase in concentration in concentration of Hydrogen Peroxide then the reaction rate of the liver catalase will decrease. IV: concentration
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How is pH affecting catalase activity? Hypothesis The catalase activity is assumed to be the most active in pH7. The higher or lower concentration away from the optimum pH of catalase‚ the slower the rate of activity is. Data Collection & Processing I collected the results of pH4‚ pH7‚ pH10 and pH13 after 2 minutes‚ and I repeated the experiment 3 times. Below is a table to show the results‚ their averages and standard deviations: | 1st time (sec) | 2nd time (sec) | 3rd time (sec)
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Sample Lab Report: Factors which Affect the Activity of the Enzyme Catalase Purpose: Must include: background information about concepts involved in the lab‚ statement of purpose of the lab identification of independent and dependent variables. A hypothesis is often not necessary or appropriate. Enzymes are proteins that speed up chemical reactions in cells. They break down molecules called substrates. Each enzyme has only one substrate that it breaks down. Enzymes are produced in
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