Determining the Effect of Varying Substrate Concentration Question: How does concentration of potatoes affect the amount of oxygen produced with hydrogen peroxide and potatoes (enzymes)? Introduction The enzyme used for this experiment is Catalase. Catalase is inside mostly any living organism which uses oxygen. Its job is to break down hydrogen peroxide‚ into oxygen and water. (Formula) 2H2O2 ---> 2H2O + O2 (lab manual). There are limiting factors which if altered‚ can alter the procedure of
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Enzyme Catalase Activity in Reaction with the Substrate Hydrogen Peroxide Abstract We performed these experiments to observe the effects of enzymes on the rate of reactions. We tested and compared the activity of the enzyme catalase on the substrate H2O2 in various states and percentages‚ and observed the absorption values of the enzyme-substrate relationship at different concentrations. Our results show that the more substrate available‚ the quicker the reaction will happen except in one test
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Practical: The Effect of the Enzyme Catalase from Chicken Livers on Hydrogen Peroxide [pic] Strand1: SA1‚ SA2‚ SA3 Date: Group Members: Marks: /12 (Part A) + /18 (Part B) = /30 INTRODUCTION: |There are many different reactions that are happening in our bodies all the time. There are reactions that build up molecules‚ | |synthesis/anabolic reactions‚ as well as reactions that break down molecules‚ catabolic reactions. Together
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OBJECTIVE: The experiment was carried out to investigate the effects of the increase in the enzyme concentration on the rate of reaction. By using self investigative and experimental skills‚ the experiment was done in order to determine how the rate of reaction will be altered‚ whether it will increase‚ decrease or remain constant when the different concentration of enzymes added. INTRODUCTION: Enzymes are produced naturally in plant‚ animal‚ and microbial cell. There are thousands of different
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transfer 10 mL of H2O2 into an unlabeled 60-mL cup. Then‚ we used a transfer pipet to add one mL of catalase solution into the unlabeled 60-mL cup that we put H2O2 in. After that‚ we observed the solution for one minute. Then we tested the effect of boiling on enzyme activity. First we used a transfer pipet to transfer 4 mL of catalase into a test tube. After that‚ we placed the test tube filled with catalase in a boiling water bath for five minutes. While we were waiting‚ we rinsed the unlabeled cup we
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conditions affect the rate at which enzymes accelerate/cause reactions In this lab students measured the height of the foam after catalysis between catalase (enzyme) and 7 other (solutions) to determine which solution had the fastest reaction rate.. The control variable of the experiment would be the solution of only hydrogen peroxide‚ water‚ and catalase. The independent variables of the experiment were the abiotic factors such as PH level‚ temperature‚ and the amount of salt within the environment
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Tittle : Investigation of the Enzymatic Effects of Materials on Hydrogen Peroxide Solution Objective: To investigates the enzymatic effect of various materials in the hydrogen peroxide solution. Table 1 Test Tube Contents with 5 cm3 hydrogen peroxide Observations before and after using wood splint Observation of after Observation of after adding hydrogen using wooden glowing peroxide splinter 1 Fresh liver Moderate
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Prac 3 The effect of change substrate concentration on the activity of an enzyme. Purpose To investigate the effect of change on the substrate concentration on the activity of an enzyme(catalase). Materials Potato‚ mortar & pestle‚ 4 test tubes‚ test tube rack‚ spatula‚ hydrogen peroxide 6%‚ 3%‚ 1.5%‚ pipettes‚ scalpel‚ ruler. Procedure 1. Cut using scalpel several raw potato cubes each measuring 1 cubic centimeter (cc). 2. Each cube was ground with a mortar & pestle and then added
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concentration of the catalase which the disk is soaked in‚ it will have a direct correlation on the rate of hydrogen peroxide being broken down into oxygen gas. Prediction: Since the rate of reaction can be lowered by the addition of catalysis such as an enzyme. Moreover to increase the effectiveness of an enzyme one must add additional amount of enzyme due to saturation. Therefore the 100% will have the greatest effect because is has the highest concentration of the catalase.
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conclusion that my bacteria was a positive cocci. After the gram stain I performed specific tests based of those results of the stain to determine what my unknown could be. The first test I did was the catalase test. It had a positive reaction meaning the bacteria produced the enzyme catalase. This helped me narrow my unknown down to Micrococcus and Staphylococcus. I inoculated my unknown onto a mannitol salt agar. The growth and color change to yellow indicated mannitol fermentation because acid
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