The Effect of Temperature on the Enzyme Catalase Stephen Francis Biology 183 Abstract This experiment was performed to determine the resultant effect of temperature change on the reaction between the enzyme catalase and hydrogen peroxide. This experiment was performed by measuring and comparing the amount of oxygen bubbles produced and the absorbance of the catalase and hydrogen peroxide solution over time at room temperature‚ 2°C‚ 50°C‚ and 60°C. The overall result of this experiment proved
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concentrated‚ which was hard to identify the shape of the bacteria‚ while other sections were less concentrated and was easier to analyze the shape. The next step according to the result‚ will be a catalase test.
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be affected by changes in something like temperature. A catalyst is a substance which changes the role or speed at which a reaction occurs. The catalyst in our experiment was the enzyme‚ catalase. An enzyme is a specialized protein which catalyzes reactions in cells. The enzyme in our experiment was catalase‚ which came from the potato juice. The function of an enzyme is to catalyze reactions in cells. The specific function of the enzyme in this experiment was to see if it affected the activity
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Temperature (⁰C) of the catalase and H2O2 Both of the solutions will be poured into test tubes/beakers and then cooled and heated to certain temperatures (10‚ 22‚ 30‚ 37‚ 45‚ and 60⁰C) and with the use of ice and a water bath ( in a separate beaker) I attempted to control the temperature of the catalase mixture as well as the H2O2 solution Dependent Variable: Rate of reaction (cm3/second) The gas syringe (± 0.5) is used to measure the amount of oxygen produced from the catalase reaction. A rubber stopper
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influence on the enzyme activity will be the subject of this investigation. Catalase of potato tuber cells will be the enzyme used in the experiment. RESEARCH QUESTIONWhat is the influence of different values of pH on the activity of catalase in potato tuber cells?VARIABLESIndependent: potato discs treatment (placing them into buffer solutions of different pH values‚ mixed with hydrogen peroxide)Dependent: the activity of the catalase‚ reflected by the rate at which oxygen is evolved‚ measured at every
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THE EFFECT OF CATALASE ON HYDROGEN PEROXIDE The aim of the experiments is to see if increasing the surface area of the enzyme Catalase‚ affects the relative activity of the substrate Hydrogen peroxide. Then to observe and measure the effect the Catalase has on the chemical breakdown of the hydrogen peroxide. My theory is if you keep increasing the surface area of Catalase‚ the more active sites are available to join with the substrate causing an increase in the breakdown of the hydrogen peroxide
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Title : THE EFFECT OF PH ON THE ACTIVITY OF CATALASE Aim : To study the effect of pH on the activity of catalase. Introduction : Catalase‚ an enzyme found in many different tissues‚ catalyses the breakdown of hydrogen peroxide into water and oxygen. 2 H202 → 2H20 + O2 Hydrogen peroxide is a toxic substance that can be formed during aerobic respiration and catalase removes this product. The activity of catalase can be measured by finding the rate of oxygen release from hydrogen peroxide
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INTRODUCTION The enzyme catalase converts hydrogen peroxide (H2O2) to water and O2. The evolution of O2 causes bubbling. Thus‚ catalase-positive organisms that are mixed into hydrogen peroxide will cause bubbling (catalase-negative organisms will not). This test is good for distinguishing between Gram-positive cocci in chains (catalase negative) versus Gram-positive cocci in clusters (catalase positive). The coagulase test is used to differentiate Staphylococcus aureus from coagulase-negative
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The Effect of catalase reaction on temperature Table of Contents: Bibliography ………………………………………………………………………………………………….. Page 1 Introduction……………………………………………………………………………………………………Page 2-3 Methods/ Procedures……………………………………………………………………………………..Page 4-5 Results ………………………………………………………………………………………………………….. Page 6 Analysis of Data ……………………………………………………………………………………………. Page 6 Graph …………………………………………………………………………………………………………... Page 7 Conclusion……………………………………………………………………………………………………
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Reaction of catalase with hydrogen peroxide AIM: I aim to find the rate of reaction between catalase and hydrogen peroxide. Enzymes such as Catalase are protein molecules that are found in living cells. They are used to speed up specific reactions in the cells. Each enzyme just performs one particular reaction so they are all very specific. Catalase enzymes found in living cells e.g. in yeast‚ potato or liver‚ speed up (in our case) the breaking down of hydrogen peroxide. The lock and key analogy…
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