Aim: To find the effect of temperature on enzymes‚ using a potato as a catalyst. The source of catalase is in the potato cells. 2H2O2 → O2 + H2O Planning: Introduction: An Enzyme is any one of many specialised organic substances‚ composed of polymers of amino acids‚ that act as catalysts to regulate the speed of the many chemical reactions involved in the metabolism of living organisms Enzymes are classified into several broad categories‚ such as hydrolytic‚ oxidising‚ and reducing‚ depending
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EFFECT OF TEMPERATURE‚PH AND SUBSTRATE CONCENTRATION ON ENZYME ACTIVITY | Aim To investigate the effect of Temperature‚Ph and substrate concentration on the rate of enzyme activity. Hypothesis
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the enzymes‚ when it happens‚ and why it denatures the way it does. b. Hypothesis Materials and Methods a. Materials 50ml beaker of fresh potato catalase Reaction chamber Ring stand and clamp 10ml graduated cylinder 100ml graduated cylinder 3% hydrogen peroxide Pan (water bath) Pipette Hot plate Ice Thermometer Boiled Catalase Buffers of varying pH: 4‚7‚10 Distilled water Balance NaCl (salt) b. Methods Refer to handout c. Statistical Methods Results a. Dependent
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factors determining a positive or negative result. Test Performed Media/Reagents Used Positive Test Observations Negative Test Observations Gram Stain Crystal violet Gram’s iodine 95% Alcohol Safranin Blue or violet cells Pink cells Catalase Glass slide 3% H2O2 Agar plates with colonies Toothpick Bubbles produced No bubbles produced OF Test 2 tubes of OF medium (glucose agar with bromthymol blue) 1 tube with sterile oil 1mL pipette Pipette and pump Colour change
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Microbiology Final exam Study reference 1. Gram Stain * Verify if bacteria are present or not. * Controls – positive (purple) – S.aureus negative ( red/pink) – E.coli 2. Endospore Stain Positive controller – B. magneterium Green spore- Positive Pink (vegetative ) – Negative 3. Acid fast Positive control – M. smeagmatis Blue – negative Pink /red- positive 4. Motility Positive control - P.vulgaris A. Non motile is negative test B. Motile is a positive test 5. Carbohydrates
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quickly denature. Hydrogen peroxide (H2O2) is naturally formed in living organisms‚ however it is very harmful and is broken down immediately by several enzymes including catalase. This enzyme catalyses the breakdown of hydrogen peroxide to water and oxygen. Persons with acatalasemia (a hereditary condition) have extremely low catalase activity and‚ although present worldwide‚ it is more commonly found in Koreans. Hydrogen Peroxide is usually used as a topical disinfectant in wounds and the bubbling
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200cm³ Liver Catalase Solution 100cm³ of following Buffer Solution – pH5 pH7 pH9 pH11 Method: The materials were collected. The test tube rack one with 4 test tubes had been labelled A to D. The 2cm³ of each buffer solution had been inserted to test tubes A to D. 3cm³ of hydrogen peroxided was introduced to the tubes then a few drops of detergent had been included to the tubes A to D. We then used test tube rack two with 4 test tubes and labelled them 1 to 4. The 2cm³ of catalase solution
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Title: Enzyme Activity Aim: To investigate the activity of the enzyme catalase in liver and potatoes‚ and to investigate the effect of temperature‚ surface area‚ pH and certain chemicals on the activity of catalase. Equipment: Dilute hydrochloric acid solution x 1ml 10 volume hydrogen peroxide x 100ml Copper sulphate solution x 1ml Aluminum nitrate solution x 1ml Zinc nitrate solution x 1ml dilute NaOH solution x 1ml Mortar and pestle x 1 25ml Beaker x 1 Hot plate x1
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Investigate Concentration of Substrate on Catalase enzyme 1. Aim/Prediction. Aim of this experiment is to investigate concentration of substrate on catalase enzyme‚ as well to see how they work in different concentrations of hydrogen peroxide (H2O2). Prediction would be‚ that the filter paper discs that were used‚ will rise up to the surface in a time in the tube of different concentration of hydrogen peroxide. And in different concentrations the time will be different. As higher the concentration
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poisonous chemicals into harmless substance. When we tested the liver with 2ml of hydrogen peroxide for a normal reaction it showed that it was exothermic. We added more hydrogen peroxide and the reaction rate of the liver was 3. We learned that the catalase is reusable because the liver reacted both times when we put in the hydrogen peroxide. We also tested another enzyme reaction for pH. In this test we learned that high pH equal high reaction rate. The 4 items tested were acetic acid (vinegar)‚ sodium
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