Catalase Experiment Research Question: How does the weight of a piece of liver (0.4g‚ 0.8g‚ 1.2g and 1.6g in this case) affect the amount of gas created after 2 minutes when combined with hydrogen peroxide? Aim: To see find out how the amount of catalase correlates with the amount of gas created when in combination with hydrogen peroxide. Materials: 15g of liver 25 ml of hydrogen peroxide 1 scale 1 bucket 1 100ml graduated cylinder 1 250ml flask with bung 1 50cm tube (able to fix onto top of flask)
Premium Oxygen Hydrogen peroxide Enzyme
Investigating How Different Concentration Effects The Rate Of Reaction. Strategy A Possible Factors * Source of catalase * Concentration * Surface Area of enzyme * Concentration of enzyme * pH * Temperature Chosen Factor We chose to investigate the concentration of enzyme as we had previously investigated the optimum temperature for catalase in the preliminary investigation. Concentration of enzyme is also fairly easy to investigate‚ as you need to only increase
Premium Enzyme PH Catalase
Biology Enzyme Catalase Investigation Aim: The aim of this investigation is to study and observe whether or not the concentration of hydrogen peroxide (varying from 10 – 30 millilitres) affects the rate of reaction. Hypothesis: With the increase of the concentration of Hydrogen Peroxide 3% substrate‚ I prediction that the rate of pressure increase will begin to amplify. The pressure is bound to increase because the catalase quickly reacts with the hydrogen peroxide; this is why the more substrate
Premium Catalase Measurement Gas
produced as a bi-product of some metabolic reactions. Hydrogen peroxide is a highly active chemical used in household products for bleaches and cleansing wounds. In a cell its build up would be highly toxic. However‚ liver cells contain an enzyme‚ catalase‚ which immediately breaks down hydrogen peroxide. It is a peroxidase and breaks up the toxic hydrogen peroxide to water and oxygen which are both nontoxic. The reaction is exothermic‚ meaning that energy is released in the form of heat. It is the
Premium Oxygen Hydrogen peroxide Enzyme
substrates. Enzymes change the rate in chemical reactions. It does this without having to change its own shape‚ which makes enzymes different from other proteins. A common enzyme that we have is catalase‚ which breaks down hydrogen peroxide into the products water and oxygen. To observe this catalase activity I used small amounts of liver samples slightly smaller than the size of a pea to see the reaction when hydrogen peroxide is added to it. In this experiment I exposed the liver samples in
Premium Temperature Chemical reaction Enzyme
balance‚ 250 ml graduated cylinder‚ and oven mitt or tongs. Methods: 1. Research (online) the reaction between catalase and hydrogen peroxide and be familiar with the reactants‚ products‚ and enzyme. You will include the overall reaction in your lab report for this experiment. We will measure enzyme activity by measuring the height of the bubbles produced. 2. Prepare the potato extract (catalase) using the following technique: - peel 3 medium size potatoes and cut them into quarters -add the potatoes
Premium Enzyme Water Catalase
Since it was gram positive‚ I knew my organism had a thick peptidoglycan and no outer membrane. After finding out my organism B was gram positive‚ I did the catalase test on it. After adding the two drops of H202 and seeing bubbles‚ I ruled out Streptococcus lactis. My organism did have the catalase enzyme; meaning it was either Bacillus subtilis or Staphylococcus. To distinguish the two‚ I performed the simmons citrate test on them. Since the tube stayed green I confirmed
Premium Bacteria Staining Gram staining
Observing Enzyme Activity Purpose: The purpose of this experiment was to test whether the pH affected the enzyme reaction rate. Hypothesis: If the enzyme is in a basic solution‚ then it will react faster because the enzyme (catalase) reacts better in basic solutions. Materials: 10 potato cubes (1 cm3) -Pipet Baking soda solution -50 ml glass beaker Bleach Water Lemon juice Vinegar 5 glass test tubes Drying rack Timer Graduated cylinder Hydrogen peroxide Procedure:
Premium PH Enzyme Catalase
Computer Enzyme Action: Testing Catalase Activity 6A H2O2 is toxic to most living organisms. Many organisms are capable of enzymatically destroying the H2O2 before it can do much damage. H2O2 can be converted to oxygen and water‚ as follows: 2 H2O2 → 2 H2O + O2 Although this reaction occurs spontaneously‚ enzymes increase the rate considerably. At least two different enzymes are known to catalyze this reaction: catalase‚ found in animals and protists‚ and peroxidase‚ found in plants. A great
Premium Enzyme PH Chemical reaction
in the body produce toxic by-products which need to be converted. The liver produces hydrogen peroxide‚ which is extremely poisonous. When the catalase comes in contact with the hydrogen peroxide‚ it is broken down into water and oxygen. Aim: The aim is to investigate and determine the effect of change in pH on the activity of the enzymes catalase. Equipment: Test tubes Test tube rack Pipettes Stopwatch Ruler Pen Safety goggles Gloves Materials: Liver pureed 3% of hydrogen
Premium Enzyme PH Oxygen