AP Biology Lab: Catalase (Enzymes) Abstract In this laboratory exercise‚ studies of enzyme catalase‚ which accelerates the breakdown of hydrogen peroxide into water and oxygen. The purpose was to isolate catalase from starch and measure the rate of activity under different conditions. The laboratory was also conducted in association with a second laboratory that measured the effects of an inhibitor on the enzymes. Changes in temperature and pH along with Substrate Concentration and Enzyme
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Potato Osmosis and Diffusion Lab Report Emily Cocq Research Question: Will there be a difference in the experimented potato cores after being subjected to high concentrations of sugar? Introduction: Water can move through protein channels in cell membrane/cell walls. The water will move due to the water potential of the cells. If there is a greater concentration of solutes (chemicals) inside the cell than outside the cell and the chemicals can not move‚ then water will respond by moving
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Rate of Catalase in Various Concentrations of Hydrogen Peroxide QUESTION: How long does the catalase take to float to the top of a cup filled with different amounts of hydrogen peroxide concentration? PREDICTION: A prediction that can be made for this experiment is that the higher the concentration‚ the faster time it takes the catalase to react with the solution. As the concentration increases from 1% to 100%‚ the average reaction rate of the catalase will decrease‚ as the catalase will speed
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Appearance of steel wool after 20 minutes From (relatively) shiny metal strips to notsoshiny strips that are stained with dark‚ reddishbrown stuff. Part II: Decomposition of hydrogen peroxide Appearance of potato and hydrogen peroxide combination: Lots of white‚ foamy bubbles around the potato slices. Day 2 Observations: Part III: Reaction between zinc and acetic acid Appearance of penny in vinegar after 30 minutes: Pretty dark coloured. Quite hard to see Lincoln’s head or the Memorial
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different sucrose solutions. The following data was observed and collected throughout the experiment. Observations: -Qualitative: The potatoes were: Tubed shaped Yellow in color Initially hard in texture -Quantitative: Table 1: Weight of the potato in grams before and after placed in a different concentrations of sucrose solutions. Sucrose solution (M) Weight Before (g) ± 0.01 (A) Weight After (g) ± 0.01 (B) 0 4.56 4.31 0.2 4.22 4.28 0.4 4.13 4.08 0.6 4.57 4.45 0.8 4.60 4
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Pseudomonas aeruginosa Pseudomonas aeruginosa is the bacteria of my initial unknown project. Determining which bacteria I had was completed by many steps. The one fact that I had‚ was that it was a gram negative bacteria because on the gram stain it appeared pink. Its cell wall is composed of a plasma membrane‚ periplasmic space‚ peptidoglycan and an outer membrane (lipopolysaccharide and protein). By looking at the agar plate it was easy to tell that it was not a swarmer and it did not have any
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Potato Lab Background information: A hypertonic solution is a concentration that is higher than the solution. A Hypotonic solution is a concentration that is lower than the solution. An isotonic solution is a concentration and solution that is the same amount. Hypothesis question: What will happen if you put a potato in hypertonic solution? What will happen if you put a potato in hypotonic solution? What will happen if you put a potato in isotonic solution? Hypothesis statement: If you
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Diffusion and Osmosis Introduction: Purpose: 1. To simulate and observe the diffusion of solutes and the osmosis of water through a semipermeable membrane through color change and sugar tests. 2. To speculate osmosis occurring in dialysis bags and potato cores by comparing percentage change in masses. Background information: Molecules are always in random‚ constant movement due to their kinetic energy. This causes the molecules of a cell to move around and bump into each other. The random movement
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substrate and maintains a firm grip‚ known as induced fit. This allows for great variations of enzymes. Abstract In this experiment‚ my lab partners and I tested how time effects a catalase reaction. The amount of hydrogen peroxide was recorded after the reaction for the certain time given has taken place. We used sulfuric acid to stop the reaction with the catalase from occurring. This process is known as denaturing (Campbell 152). The potassium permanganate in this experiment was used as hydrogen
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Osmosis and Diffusion Lab Observations: Diffusion: | Before | After | Color of liquid sack | clear | blue | Color of liquid in beaker | dirty yellow | golden | Glucose present Stick Test | no | yes | Color of the test stick | No change in color | Green | Osmosis: | Fresh | Salt | Beginning mass of potato | 2.51 | 2.16 | Texture of potato | Dry | Dry | Overnight mass | 3.12 | 1.84 | Overnight Texture | Hard‚ strong | Squishy‚ pliable | Data and Analysis: Diffusion:
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