Purpose: The purpose of this lab was to observe and understand the effects of changes in temperature‚ pH‚ enzyme concentration‚ and substrate concentration on the reaction rate of an enzyme-catalyzed reaction. Another purpose of the lab was to explain how environmental factors affect the rate of enzyme-catalyzed reactions. Hypothesis: I believe that if there is an increase in enzyme concentration‚ an increase in temperature‚ or an increase in pH‚ then the intensity of the reaction will
Premium Chemistry Chemical reaction Enzyme
catalyzes the substrate molecule into a product. Enzymes have the capability to break or build compounds which keep cell systems functioning. For example‚ our digestive tract has catabolic enzymes which break apart food for storage and reuse. Our lab experiment studied the relationship of benzoquinone production when altering the pH and temperature of the reaction‚ as well as enzyme and substrate concentrations. We used a Spec 20 to evaluate absorbance at 540 nm. Absorbance represents rate of
Premium Enzyme
I have been asked to investigate the effects of osmosis on potato cell sap. I will test potato cylinders in different strengths of sucrose solution and record any results in a table. I will then analyse my findings and work out the isotonic point of the potato cell sap. Prediction I have looked at my scientific knowledge from the background theory I carried out and I will base my prediction upon this information. In this experiment‚ I expect the different concentrations to show fairly noticeable
Premium Concentration Sucrose Measurement
Purpose: To find out the densities and to find out the name of the unknown metals. (Based on the extensive and intensive properties) Check up the words mass‚ volume‚ density‚ extensive properties‚ and intensive properties. Where do the units for mass and volume) come from and what do they mean? What is the density of distilled water? What is Archimedes principle? Does temperature affect the density of a solid? Liquid? Gas? Materials: Safety glasses‚ 10‚ 25 or 50 mL graduated cylinders
Premium Water Liquid Density
Unknown Lab Report April 25th‚ 2006 Introduction The purpose of this lab was to identify two unknown bacteria cultures using various differential tests. The identification of these unknown cultures was accomplished by separating and differentiating possible bacteria based on specific biochemical characteristics. Whether the tests performed identified specific enzymatic reactions or metabolic pathways‚ each was used in a way to help recognize those specifics and identify the unknown cultures
Premium Enzyme Staphylococcus Staphylococcus aureus
The Effect of pH on the Rate of Enzyme Catalysis of Catalase Objectives: The objective of this lab was to develop a protocol to investigate the effect of an environmental variable on the catalytic function of an enzyme. More specifically‚ the objective was to perform an experiment in order to test the effect of pH on the function of the enzyme catalase. Introduction: Enzymes are proteins that act as catalysts for reactions. This simply means that enzymes lower the activation energy required
Premium Enzyme Chemical reaction PH
Potato Osmosis Biology SL - ATh INVESTIGATION “Potato Osmosis” INTRODUCTION Osmosis is a process that occurs at a cellular level that entails the spontaneous net movement of water through a semi-permeable membrane from a region of low solute concentration to an area of high solute concentration in order to equalize the level of water in each region. Involved in this process are hypotonic‚ hypertonic and isotonic solutions. A hypotonic solution is one with a lower osmotic pressure‚ indicating
Premium Osmosis
Biological Macromolecules Lab Cells‚ the fundamental units of life‚ are composed of various combinations of organic macromolecules: carbohydrates‚ lipids‚ proteins‚ and nucleic acids. This lab exercise is designed to show you the qualitative tests commonly used to detect their presence. Objectives Test for the presence of monosaccharides by using the Benedict’s test Test for the presence of starch‚ a polysaccharide‚ by using the Lugol’s iodine test Test for the presence of lipids by using the
Premium Carbohydrate Glucose Polysaccharide
salinity of solutions has on the process of osmosis and net weight gain/ loss by potato cells. This would be done by using similar size potato cubes and covering them with different concentrations of saline solutions (0.5% and 2.0%) in beakers then measuring the change in weight of the potato cubes. If the salinity of the solution is high then the weight of the potato cubes will decrease. Materials • 9 X 2cm2 potato cubes • 3 X
Premium Osmosis Concentration Chemistry
Investigation on the effects of Temperature‚ pH levels‚ and Enzyme Concentration on the reaction rate of the Decomposition of Hydrogen Peroxide by Catalase The purpose of this investigation is to figure out how temperature‚ pH‚ and enzyme concentration affect the reaction rate of an enzyme. It’s important to understand how certain factors affect enzymes because of their crucial role in the metabolic processes of life. Enzymes lower the activation energy necessary for a chemical reaction to
Premium Enzyme Metabolism