The Catalase Lab Stephen Human Anatomy & Physiology 9/30/12 Problem- How do different environments affect the reactivity of catalase? Hypothesis- If more catalase is added then more oxygen (kPa) will be produced in a faster rate because there is more catalase to react upon. If less catalase is added then less oxygen (kPa) will be produced in a slower rate because there is less catalase to react upon. Variable- Independent- Amount of Catalase (Filter Paper) Dependent- Amount of
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Arnesh Kaewloyma 9/30/14 Enzyme Catalase under optimum conditions‚ speeds up the decomposition of hydrogen peroxide The students have studied that Hydrogen peroxide is a jeopardy to metabolism if it’s not obliterated. Enzymes are proteins produced by living cells of that act as catalysts which affects the rate of biochemical reaction by speeds up the breakdown of hydrogen peroxide into water and oxygen gas. The students have formed a hypothesis toward this experiment. If the speed of
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the bacterium was a gram negative or a gram positive. After performing the gram stain‚ I concluded that by the appearance of purple spherical clusters resembling grapes that is was a gram positive cocci. A Catalase test was then performed using hydrogen peroxide. A positive catalase test was observed when bubbles started to form on the culture distinguishing Staphylococcus spp. form on the culture distinguishing Staphylococcus spp. from Streptococcus spp. Finally‚ a Mannitol Salt Agar
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decreasing the energy of activation. The main function of enzyme catalase is to convert hydrogen peroxide (H2O2) in our bodies into oxygen and water. This can be visually seen when hydrogen peroxide is put on a wound and the peroxide bubbles. Enzymes can also be found in plant cells and fungi. (Huston.) In this experiment we test the many variables that can change the rate of this reaction such as temperature‚ concentration levels of enzyme catalase and pH values. We are able to track these changes using
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Abstract: Enzymes‚ molecules that speed up chemical reactions‚ are specific to one substrate. In this experiment the substrate hydrogen peroxide and the enzyme catalase will be used. The higher the concentration of potato extract‚ or catalase‚ the faster the reaction and the more substrate present will result in a decrease in the time of the reaction. The amount of concentrations of enzymes and substrates are changed to determine if the reaction is further catalyzed by a greater concentration of
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Enzyme Activity and Oxygen Production Throughout this report you will gain information as to how temperature effects the amount of oxygen produced in an enzyme- catalase experiment. In the experiment we used liver extract as a catalase and created a chemical reaction within a reaction chamber between the catalase and hydrogen peroxide as well as three different controlled temperatures. In the procedure below there will be a step by step process as to how the experiment was created. As
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Effect of Temperature ( C ͦ) on Enzyme Catalase Activity in potato Aim: To investigate the Effect of temperature (10‚ 37‚ 60) Celsius (C ͦ) on enzyme catalase activity in potato using 2% of hydrogen peroxide (H202) as the substrate measuring the height (cm) of oxygen gas (bubbles) and calculating the volume of oxygen bubbles produced (cm3) Introduction: Enzymes are biological catalysts that speed up metabolic reactions without being affected. They lower the activation energy needed to start
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Osmosis Lab Introduction: In order for cells to interact with their environment‚ molecules must be able to move through the cell membrane. Movement within the cell occurs by diffusion. Molecules move through the cell membrane by osmosis. Diffusion is the movement of molecules from a region of higher concentration to a region of lower concentration. This happens because of random molecular motion. Molecules move around randomly until there is an even mixture throughout cell and mixture. The overall
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Retrieved October 29‚ 2013‚ from http://prezi.com/wii0fgw48euc/potatoe-enzyme-lab/ What enzyme is present in liver and potatoes. (n.d.). Retrieved October 29‚ 2013‚ from http://wiki.answers.com/Q/What_enzyme_is_present_in_liver_and_potatoes#slide2 What Is the Optimum PH for Catalase - Ask.com. (n.d.). Retrieved October 29‚ 2013‚ from http://www.ask.com/question/what-is-the-optimum-ph-for-catalase What is the optimum PH for potato catalase and why? - Yahoo! Answers. (n.d.). Retrieved October 29‚ 2013‚ from
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The purpose of the lab was to investigate osmosis‚ which was successfully achieved because there was a change in the potato’s mass which was due to the net movement of water either into the potato cells or out of the potato cells. In the 0.400M solution‚ the potato decreased in mass‚ this was due to the reason that the solution was hypertonic. There was a higher concentration of solute and lower concentration of water in the solution than there was in the potato cells. This led the water to travel
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