Abstract The aim of this experiment was to investigate the effect of temperature on the enzyme catalase. The original research question was exploring the effect temperature would have on a yeast catalase reacting with hydrogen peroxide (H2O2). To address the latter question a series of experiments were conducted. The various temperatures experimented with were as follows: 22 degrees Celsius (room temperature)‚ 0 degrees Celsius (freezing)‚ 100 degrees Celsius (boiling)‚ and 37 degrees Celsius.
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crystal violet for 30 seconds. Afterwards‚ it should be gently rinsed off with distilled water and be covered again with Gram’s iodine for 10 seconds. Then gently rinse off the Gram’s iodine with distilled water and decolorize it with ethanol. The ethanol should be rinsed off with distilled water again and then be covered with safranin for 30 seconds. The last step would be to rinse off the safranin with distilled water and blot it dry. The results of the bacterium was stained purple‚ indicating that it
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The Effect of Temperature on Enzyme Activity and Oxygen Production Throughout this report you will gain information as to how temperature effects the amount of oxygen produced in an enzyme- catalase experiment. In the experiment we used liver extract as a catalase and created a chemical reaction within a reaction chamber between the catalase and hydrogen peroxide as well as three different controlled temperatures. In the procedure below there will be a step by step process as to how
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Substrate concentration and yeast catalase Aim: To see how the substrate concentration in hydrogen peroxide affects the rate of an enzyme controlled reaction using yeast catalase. Introduction: An enzyme is a biological catalyst made of protein. Enzymes are protein molecules found in living organisms and in this case I will use a yeast catalase. Catalase is an enzyme that catalyzes the reduction of hydrogen peroxide. Hydrogen peroxide is a poisonous by-product of metabolism‚ so it is very
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REDUCTION Oxygen makes up only about 20% of the air‚ yet is the essential component for so many reactions. Without it fuels would not burn‚ iron would not rust and we would be unable to obtain energy from our food molecules through respiration. Indeed animal life on the planet did not evolve until a certain concentration of oxygen had built up in the atmosphere over 600 million years ago. The term oxidation has been in use for a long time to describe these and other reactions where oxygen is added
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graph shows that the highest rate of reaction is at pH 7. This therefore supports my theory that at pH 7 the rate of reaction will be highest. This relate to other resources‚ such as books and the internet‚ which show that the optimum pH value for catalase is pH7‚ therefore there is no difference between the optimum pH and the pH used in the experiment‚ hence the enzyme structure is not affected by the pH and is most active. However the graph inadequately supports the second part of the prediction
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Catalase Lab Purpose: To observe the effects of hydrogen peroxide on living tissue and non-living materials‚ showing the presence or absence of catalase as observed by the presences of bubbles. Hypothesis: If we pour H2O2 on certain substances‚ the catalase will break down and bubbles will appear at the surface. Data table: Name | Time | Observation | Raw Potato | 1 min. 30 sec. | Slow reaction‚ barley any bubbles. | Raw Liver | 5 min. 30 sec. | Extremely fast reaction‚ very foamy‚ Big
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On the periodic table‚ oxygen is the 8th element. It is located in period 2 and group 16. Oxygen is classified into many things. For example‚ on the periodic table‚ oxygen is classified as a nonmetal gas with an atomic number of 8 and atomic weight of 15.9994. In addition‚ it has 6 valence electrons. The symbol of oxygen is O and oxygen has 8 protons‚ 8 neutrons‚ and 8 electrons. The electron configuration of oxygen is 1s2‚ 2s2‚ 2p4. Oxygen had been produced by several chemists prior to its discovery
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to maintain the internal environment [2]. Hydrogen peroxide is an oxidizing agent that can be decomposed by catalase [11]‚ the liver is able to decompose hydrogen peroxide enzymatically because it contains catalase [12]. The reaction rate of an enzyme catalysed reaction under the effect of changes in temperature was tested in this experiment. It was hypothesised that the fastest reaction rate would occur a 37 degrees Celsius. Liver and hydrogen peroxide were placed into 4 different test tubes‚ all
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of 1% hydrogen peroxide solution was made. In order to get this‚ the stock solution‚ which was 3%‚ was diluted using 10 mL of hydrogen peroxide and 20 mL of water. In the second beaker‚ a ¼ dilution of potato extract was made using 2 mL of potato extract and 6 mL of water. The third plastic beaker contained 8 mL of water. Using the first and second beaker the experimental assay was performed. Using forceps a small paper disk was dipped into the second beaker containing the potato catalase for
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