Identifying sites of protein synthesis in Chlamydomonas using erythromycin and cyclohexamide as protein synthesis inhibitors. October 16‚ 2009 Introduction: In living cells‚ prokaryotic or eukaryotic‚ the synthesis (construction) of proteins is accomplished by similar machinery. Amino acids‚ ribosomes‚ messenger RNA (mRNA)‚ and transfer RNA (tRNA)‚ are all necessary for the building of functional proteins in a cell. Ribosomes are the site of protein synthesis in a cell‚ and there are two
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Sarah Khan Bivins-5th April 20th‚ 2013 Protein Synthesis Every day‚ you take in an abundance of different biomolecules; one of them being proteins. Have you ever wondered how proteins are made? They don’t just grow from a tree or fall from the sky‚ they are made through a process called protein synthesis. Protein synthesis is broken up in two two steps: transcription and translation. Transcription starts inside the nucleus when the DNA is unzipped by helicase. Following that‚ the mRNA nucleotides
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Question 5: The chromophore in this assay is Coomassie Brilliant Blue dye. Question 6: It is important to set up a blank to separate the solute (saline) from the protein (stock). By subtracting the absorbance of the blank (which has no protein present) from the original absorbance the absorbance of the protein at each concentration will remain. Question 7: The Lowry method relies on two different reactions. The first is the formation of a copper ion complex with amide bonds‚ forming reduced
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Experiment No. 7 HYDROLYSIS AND ANALYSIS OF CARBOHYDRATES Methodology Materials: |1% solutions: | conc. H2SO4 | |glucose‚ fructose‚ maltose‚ sucrose‚ lactose‚ |Molisch reagent | |agar-agar‚ gum arabic‚glycogen‚ cotton‚ |I2 in KI solution (Lugol’s
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Denaturation of proteins involves the disruption and possible destruction of both the secondary and tertiary structures. Since denaturation reactions are not strong enough to break the peptide bonds‚ the primary structure (sequence of amino acids) remains the same after a denaturation process. Denaturation disrupts the normal alpha-helix and beta sheets in a protein and uncoils it into a random shape. Denaturation occurs because the bonding interactions responsible for the secondary structure (hydrogen
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The main purpose of carbohydrate loading is to reestablish both the glycogen of the muscles and liver in order to keep hunger away during some types of competitive sports. These types of competitive sports include: marathons‚ long distance swimming‚ and cycling. Carbohydrate loading is done anywhere from a week out from a competition or event to only twenty four hours before. There are three main benefits to carb loading for athletes of sports like marathons‚ long distance swimming‚ cycling‚ and
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17.1 Genes specify proteins via transcription and translation * George Beadle and Edward Tatum worked together with mutated (Neurospora crass) bread mold to figure out that they were missing a specific enzyme (gene) that catalyzed and synthesized a pathway required. They concluded that they were missing that enzyme because it was lacking the amino acid that coded for the enzyme‚ thus was mutated and incapable of growing. Led to the one enzyme-one gene hypothesis. The Products of Gene Expression:
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BASICS OF INTEREST RATE SWAPS A swap is an agreement between two institutions to exchange future cash flows. Suppose there is a bank receiving fixed 8% return on 2 year money lent. This bank can swap its revenue stream against another firm’s variable rate 2 year money. In practice swaps tend to be more complicated and can involve more simultaneous variations of interest rates and currency as well. When settled‚ only the differential between the would-be payments are exchanged between two parties
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Does the PH level of acids in our stomach affect the digestion proteins? Yes‚ if the acidity in my/a stomach is high in terms of the PH balance then the proteins in our stomach will be digested faster because acids are known to breakdown and eat away at many objects. The purpose of this project was to observe how the digestion of protein varies at different pH levels. This project in my eyes helps society by finding the best types of food (i.e. ones with higher/ lower acidity and pH balance)
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Investigation of Action of Saliva and 3 M Hydrochloric Acid in Two Carbohydrate Solutions Title : Investigation of Action of Saliva and 3 M Hydrochloric Acid in Two Carbohydrate Solutions Objective : To investigate the action of saliva and 3 hydrochloric acid in two carbohydrate solution Results Table1: Observation Conclusion Solution A Benedict’s test: Blue coloration turned to brick red precipitate. Reducing sugar is present in the solution A. Iodine test: The coloration remained
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