CHARACTERIZATION OF PROTEINS Abstract Different techniques and principles for protein extraction and characterization were demonstrated in this experiment. Various proteins were extracted from different sources: 1.67 g yeast invertase‚ 1.03 g egg white albumin‚ and 5.15 g of milk casein. Activity assay for invertase was performed using Benedict’s test and the enzymes inverting action on sucrose was confirmed. Warburg-Christian Method and Bradford Assay were also employed to determine the protein concentration
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The purpose of the lab was to measure the effectiveness of antacids at neutralizing excess stomach acid. A neutralization reaction is when an acid and a base react to form water and salt. To illustrate‚ H+ hydrogen ions from the acid react with the hydroxide ions OH- from the base. Antacids are taken to relieve heartburn or indigestion caused by excess stomach acid. For instance‚ antacids such as alka-seltzers contain baking soda which is highly reactive with acids‚ settling the discomfort of indigestion
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happens between an analyte and a titrant. A redox titration is done just as a normal titration is done‚ however instead of titrating an acid against a base‚ an oxidizing agent is titrated against a reducing agent and the equivalence point is reached when the reducing agent is completely oxidized by the oxidizing agent. (Chang‚ 2011). An indicator is used just as in an acid-base titration‚ although in this experiment because of its self-indicating property KMnO4 the titrant‚ it is also used as indicate
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large to penetrate the molecular weight cut off pores and no simple diffusion can occur. So it seemed like the easiest way for a solute to pass through a semipermeable membrane was‚ if it either was small enough to pass or had some sort of carrier protein that helped it along. We expected to see continuous results that do not have much difference in the five experiments that are to be performed. Experiments were conducted in order to gain a better understanding of a cell’s selectively permeable membrane
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During the lab experiment six different test were conducted. Each test consists of chemicals to be tested using 3 to 10 drops of the additive in order to observe a change. With any experiment you compose a hypothesis; where you make an educated guess. The hypothesis will primarily be‚ which base chemical will produce the greatest change based on the additive. Experiment 6.1 (Starch) when adding the three drops of Iodine to each tube observe the color change. When performing this test the chemical
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Submit your completed lab report to the Lab: Photosynthesis Lab assignment link for grading. For information on how this assignment will be graded‚ please visit the Course Information sectionChlorophyll and Accessory Pigments A pigment is any substance that absorbs light. The color we see comes from the wavelengths of light that reflect. Chlorophyll‚ the green pigment common to all photosynthetic cells‚ absorbs all wavelengths of visible light except green. The green reflects back to our eyes
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Biology 160 Lab Manual Tacoma Community College Last update 06/03/2013 This page left intentionally blank Table of Contents Table of Contents i Laboratory 1: Principles of the Scientific Method 4 In-Lab Report Sheet 12 This page has been left intentionally blank 27 Laboratory 2: Scientific Methodology & Enzyme Activity 28 In-Lab Report Sheet 28 Objectives 33 Introduction 33 Parts of the Swift M5 Microscope 33
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Protein Monique Rowland Nutrition / 241 Instructor: AN-CHIAN CHEN October 4‚ 2013 Protein All proteins are molecules that are the boosters of living organisms and are part of each organ‚ tissue‚ and cell within the human body. The protein in consumed food turns into amino acid. The amino acid is used later replinish the proteins used. Proteins have two structures. the first and primary structure of protein is the sequence of the amino acid in the protein (Kim‚ 2005). The amino acid
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that some mitochondrial proteins stimulate apoptosis in the cells (Wang‚ 2001). This research has aided in finding cure for cancer and Parkinson’s disease among many other diseases (Labbe et al.‚ 2008). Also‚ many drugs produced contain drug classes that can inhibit the function of mitochondria by preventing or altering a stage in ATP production in the Krebs cycle or electron transport chain or acting as an inhibitor of an enzyme that carries out a certain reaction in citric acid cycle or ETC (Dykens
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Science Lab- Determining the Most Effective Antacid Robby Grewal SNC2D0 Introduction: Heartburn is a form of indigestion by acid digestion it affects many people worldwide. A solution to defeat heartburn is to take an antacid. An antacid is medicine which neutralizes your stomach acidity. In this experiment‚ we will be using three liquid antacids which are Phillips Milk of Magnesia‚ Life Brand Milk of Magnesia and Diovol plus AF and determine which one is the most effective at neutralizing
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