Tatum Student ID: 4593119 Date: 7 Dec 2014 Course and Section Number: SCIN131 A004 Fall 14 Lesson 4 Lab: Chromatography and Ionic versus Covalent Bonds PART 1 Begin by viewing the following Thinkwell video 15.1.3 CIA Demonstration: Chromatography After you watch the above video‚ answer the questions below in sufficient detail: (a) (3 pts.) This video discusses 3 different types of chromatography. List each one mentioned‚ and describe their differences in as much detail as possible (your points
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Column Chromatography of Plant Pigments Jaybee Balilea‚ Sharmaine Baysic‚ Maria Anjelette Patricia Belen 3Bio-7‚ Department of Biological Sciences‚ University of Santo Tomas‚ Manila‚ Philippines Abstract Column Chromatography is a form of solid-liquid adsorption chromatography and depends on the essential principles as does in thin layer chromatography. It was used in this experiment in separating and analyzing the different components of Capsicum frutescens (siling labuyo) with the use
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5.05 Mixtures and solutions Lab report Ink Chromatography Chromatography is also used to compare and describe chemical substances. The chromatographic sequence of sorbed substances is related to their atomic and molecular structures. A change in a chemical substance produced by a chemical or biological reaction often alters the solubility and migration rate. With this knowledge‚ alterations or changes can be detected in the substance. Chromatography serves mainly as a tool for the examination and
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Introduction Flash-Column Chromatography is another useful technique used to separate and purify complex mixtures of compounds. In this experiment‚ TLC is used to determine the best solvent system for the column. Due to Flash-Column Chromatography1 being an inexpensive technique‚ many scientist use this process for drug and natural product purification. Method By using the information from Experiment 6B‚ the TLC solvent system used was 30% EtOAc: 70% Hex. Experiment 7 was broken into two parts‚
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EXPERIMENT 5: CHROMATOGRAPHY Abstract Paper Chromatography uses a chromatogram paper as its stationary phase and the solvent as the mobile phase. Retention factor is the ratio of the distance travelled by the sample to the distance travelled by the solvent. This experiment aims to separate organic compounds‚ to compute Rf values and to identify unknown compounds using Rf values. The ten samples underwent paper chromatography to determine the components of the unknown sample. The mobile phase allowed
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Paper chromatography Presented by – Miss. Shruti Vilas Kharat. SYBSc / FS13164 History- Paper Chromatography (PC) was first introduced by German scientist Christian Friedrich Schonbein (1865). PC is considered to be the simplest and most widely used of the chromatographic techniques because of its applicability to isolation‚ identification and quantitative determination of organic and inorganic compounds. Definition- Paper chromatography is an analytical method technique for separating and
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by Laboratory Reagents‚ Legacy Product Code: S/0380/48‚ CAS Number:8047-15-2) were powdered and analyzed as potassium bromide (KBr) pellets using FTIR (Model-JASCO FT/IR 4100 LE‚ made in Japan; Range: 4000-400 cm-1) Prabhu et al. (2013). Gas Chromatography–Mass Spectrometry (GC–MS) analysis: Acid hydrolysis of saponin was carried out according to the method described by Metwally et al. (2012) with some modification. Saponin was hydrolyzed with 2 N HCL (6 hr at 100 o C) under reflux‚ the residue
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Chromatography Abstract Paper chromatography is one of the methods under chromatography‚ it can use in identifying unknown compounds using known compound and it can also use as a separation technique based on the differences in affinities of components of the mixture to a stationary phase and a mobile phase. In the experiment‚ the stationary phase was the filter paper onto which the dye samples were dropped onto while the mobile phase was the solvent mixture containing ethanol and water which
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In this lab‚ there was a comparison between accuracy and precision of densities measured by volume. Mass measurements are often used units in the metric system. Volume can be examined a borrowed unit. The volume measurements were made in mL. Every measurement had a level of hesitancy affiliated with it. The experiment could feel uncertain from the object used for measurement and from the accuracy of the person doing the measuring of the volume. Because of this uncertainty‚ the experiment must be
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trending and new yet known by some people. At this this stage consumer’s demand for more goods increases therefore‚ it will result in a positive outcome‚ meaning sales would increase and profit margins will
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