Lab Report Factors That Affect Enzymes Reaction Rate Name of lab: Effects of temperature‚ pH‚ Enzyme Concentration‚ and Substrate Concentration on Enzymatic Activity Introduction: Enzymes are the most important types of proteins‚ they act as catalysis (speed up chemical reactions). If enzymes didn’t exist‚ biochemical reactions would act to slowly and they couldn’t keep up with the metabolic functions. Enzymes have a three-dimensional structure that is really complex. This structure consists
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temperature on reactions between the enzyme catalase found in animal tissue with the substrate H2O2. The hypothesis stated that an increase in the temperature of the substrate would create a subsequent increase in the rate of reaction between the enzyme and the substrate. This hypothesis was tested by immersing 1cm cubes of animal tissue (sheep liver) which contained the enzyme catalase into the substrate (H2O2 ) mixed with detergent which foamed showing a visual display of the reaction. After 10 seconds
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Enzymes are globular proteins that are synthesized by the ribosomes in a cell. They act as catalysts during biological reactions; therefore‚ enzymes are able to speed up these reactions without undergoing a permanent change themselves. These proteins are able to do this by lowering the activation energy of a reaction. To add on‚ enzymes require specific conditions under which they can work best. Reactions occur at faster rates when the temperature is higher. However‚ the rate of an enzyme-catalyzed
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Running head: LAB 5: INVESTIGATING AN ENZYME-CATALYZED REACTION Lab 5: Investigating an Enzyme-Catalyzed Reaction September 24‚ 2014 Principles of Biology 120.601 Mrs. Annemarie Duncan Abstract: (Burmania) This experiment was performed in order to examine ways in which a potato catalase enzyme reacts to various assays with differing variables. To do so a baseline assay (undiluted extract and room temperature H2O2) was used within the experiment with only one other variable changed in
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How Enzyme Concentration can Affect Rate of Reaction The purpose of this investigation was to see how the concentration of an enzyme affected the rate at which a substance was broken down. We did this by using a white protein called casein. Casein is found in milk powder‚ it is a protein and used mainly as a binding agent in foods‚ because it is mad to proteins and joins to a phosphoric acid it belong to a group called the phophoproteins. In terms of in milk it is said to be healthier if it is
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The purpose of this review paper is to discuss the effect of temperature on enzyme- catalyzed reactions. This is relevant because many diseases can be diagnosed and controlled by the processes of enzyme activity (Worthington 2015). If more information is not found about enzyme activity and how it is affected‚ many diseases may go undiagnosed and uncontrolled. Temperature is a type measurement that does not only consist of heat. It is the measurement of the mean kinetic energy of any group of particles
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that the enzyme concentration will affect the rate of reaction but only up until a certain point because there is a limited supply of substrate. Enzymes are protein molecule that acts as biological catalyst by increasing the rate of reactions without changing the overall process. They are long chain amino acids bound together by peptide bonds. Enzymes are seen in all living cells and controlling the metabolic processes in which they converted nutrients into energy and new cells. Enzymes also help
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Inhibitory and Non-Inhibitory Competition‚ Enzyme-Substrate Concentration‚ Along with Varying Temperature and pH-Balanced Environments on the Enzyme-Catalyzed Reaction of pNPP Abstract: Introduction: Many of the chemical reactions‚ which take place in in living things are controlled by enzymes. In such cases‚ the enzyme is a protein in the cell which lowers the activation energy of a catalyzed reaction‚ which serves to increase the rate of the reaction. Alkaline phosphatase is made throughout
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Factors that Affect the Rate of Reaction of Peroxidase Purpose: To determine the effect of various factors on the rate of reaction between an enzyme and its substrate‚ and also to determine the optimal ranges under which the enzyme activity is maximized. Also to determine whether saline and alcohol are inhibitors or activators Hypothesis: PH factor prediction: I predict that as the pH increases so the activity of the enzyme will increase until it reaches optimum pH range (pH 7) because the
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Purpose: To determine the effect of various factors on the rate of reaction between an enzyme andits substrate‚ and also to determine the optimal ranges under which the enzyme activity ismaximized. Also to determine whether saline and alcohol are inhibitors or activators Hypothesis: PH factor prediction: I predict that as the pH increases so the activity of the enzyme willincrease until it reaches optimum pH range (pH 7) because the enzyme is less denaturedwhen it reaches the preferred pH level
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