England Buffalo High School Abstract In this lab‚ the Beer-Lambert Law is tested by using a spectrophotometer. This is a quick and easier way to determine the concentration of a solution. Keywords: Beer-Lambert Law‚ Spectrophotometer‚ concentration Beer-Lambert Law The Beer-Lambert-Law states that there is a direct correlation between the concentration
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phosphate solution using a pH meter and construct a titration curve of an amino acid to determine the pka values of its ionisable groups to identitfy an unknown amino acid. Method: The ratio of [HPO42-] to [H2PO4-] required to produce buffer solutions at pH values 5.9‚ 6.9 and 7.9 were calculated. 0.1M of H2PO4- and 0.1M HPO42- were used to mix appropriate volumes to 25mL of each of the buffer solutions. The calibrated pH meter was used to measure and record the pH of each buffer solution and then
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Biochem. J. (1995) 305‚ 17-20 (Printed in Great Britain) 17 RESEARCH COMMUNICATION The effect of low temperatures Nicole MORE‚ Roy M. DANIEL* and Helen H. PETACH on enzyme activity Thermophile Research Unit‚ University of Waikato‚ Private Bag 3105‚ Hamilton 2001‚ New Zealand The stability of two enzymes from extreme thermophiles (glutamate dehydrogenase from Thermococcales strain ANI and f‚- enzymes‚ glucosidase from Caldocellum saccharolyticum expressed in Escherichia
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L angmuir 1995‚11‚ 2344-2347 2344 Coating Polystyrene Particles by Adsorption of Hydrophobically Modified Dextran C . Fournier‚ M. Leonard‚* I. Le Coq-Leonard‚ and E . Dellacherie L CPM‚ URA C NRS 494‚ ENSIC B.P. 4 51‚ 54001 Nancy‚ France Received March 14‚ 1995. I n Final Form: May 22‚ 1995@ I n order to decrease their hydrophobicity and thus t o reduce the protein adsorption at their surface‚ polystyrene-divinylbenzene (PS-DVB) particles have been coated with dextran. To favor its adsorption
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[OH-] in 0.025 M HCl ? 3. What is the pH of 0.50 M Sr(OH)2 ? 4. The pH of an aqueous solution is 10.32. The [OH-] is …………………… 5. The [H+] in 0.050 M Mg(OH)2 at 25oC is ………………………. 6. The pH of 0.20 M HNO3 is ……………………. 7. A solution is prepared by adding 200. mL of 0.125 M of HCl to a 500.0mL volumetric flask and filling it to the mark with water. The pH of this solution is ………. 8. Consider the equilibrium for an indicator: Hind(aq) + H2O(l) ⇌ H3O+
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vinegar solution has a H3O+ concentration of 0.015 M. What is the pH of the solution? a) 1.8 b) -1.8 c) 0.015 M d) 3.3 e) -3.3 2. What is the pOH of the solution in Problem #1? a) 10.7 b) 17.3 c) 12.2 d) 15.8 e) 7.0 3. The Ka of acetic acid is 1.8 x 10-5. What is its pKa? a) 1.8 x 10-5 b) 5.18 c) 4.74 d) -4.74 4. What is the pH of a 0.100 M solution of acetic acid? a) 1.34 x 10-3 b) 2.87 c) 1.00 d) 4.74 e) 3.74 5. What is the acetate ion (Ac- or CH3COO-) concentration of the solution in Problem
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and measure the enzyme activity using spectrophotometer respectively. For Experiment 5‚ a mixed solution of ONPG solution and Sodium phosphate buffer pH 7.3 is incubate at different temperature‚ 25‚ 40‚ 50‚ 60 and 70 °C. The mixed solution is then incubated for another 10 minutes after the enzyme is added in their respective temperature. Then‚ using spectrophotometer‚ the absorbance in the mixed solution incubated in different temperature is recorded. For Experiment 6‚ 5 different
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and heating the solution to boiling point. Upon reaching just below boiling point‚ decolorizing carbon was added to the solution‚ and the solution was allowed cooled for 2-3 minutes. The dark black solution was filtered through a gravity filtration system‚ leaving dark residue behind on the filter paper‚ and colorless to slightly pink liquid in the beaker. Then‚ 9 mL of sodium acetate buffer‚ to maintain a relatively constant pH and 1.8 mL of acetic anhydride were added to the solution‚ and then it
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chloride hydrate (MW 371.81 Da)‚ poly vinyl alcohol (MW 89‚000 Da) and 15 and 50 mL Corning centrifuge tubes were purchased from Aldrich (St. Louis‚ MO‚ USA). Acetone was purchased from Fischer Scientific Laboratory (Fair Lawn‚ NJ‚ USA). Phosphate buffer pH 7.0 was purchased from EMD Chemicals Inc. (Gibbstown‚ NJ‚ USA). 0.2 micron syringe filters were obtained from Millipore Corporation (Carrigtwohill‚ Ireland). 1.1. Collection and preparation of crude methanolic plant extract (CMPE) of Callistemon
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ml Beaker 1 container of parafilm 1 set of 20 spec tubes 1 regular test tube rack 1 small test tube rack 1 box Kimwipes Eye Droppers 1 thermometer 2-10ml Graduated Cylinders 1 Spectrophotometer 37 °C waterbath with test tube racks Solutions: 1 flasks of pH 7 buffered ONPG 1 flask of Lactose 8% 1
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