actual concentration of an acetaminophen elixir. Five standard solutions of acetaminophen and an acid buffer solution were created and analyzed with a potentiostat to determine the peak anodic voltage and current during the scanning process. Three solutions of Tylenol®—containing acetaminophen—and acid buffer were then created and scanned at the same scan rate as the five standard solutions. Additionally‚ the most concentrated standard solution was scanned at five different scan rates (between 100 and
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OBJECTIVE: The experiment was carried out to investigate the effects of the increase in the enzyme concentration on the rate of reaction. By using self investigative and experimental skills‚ the experiment was done in order to determine how the rate of reaction will be altered‚ whether it will increase‚ decrease or remain constant when the different concentration of enzymes added. INTRODUCTION: Enzymes are produced naturally in plant‚ animal‚ and microbial cell. There are thousands of different
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Abstract A buffer is a solution that resists changes in pH when H+‚ OH-‚ or H20 is added. By using standard lab equipment‚ a lab pro diagnostic tool‚ and acidic and basic solutions‚ the pH can be found. By recording the pH while adding a base or an acid gradually to a buffer solution you can find the capacity of each buffer to resist drastic changes in pH. The best buffers will keep a solution from becoming either too acidic or basic with the addition of a strong base or acid. Introduction The
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Huganir RL (1996) Interaction of the N-methyl-D-aspartate receptor complex with a novel synapse-associated protein‚ SAP102. J Biol Chem 271: 21622-21628. 12. Wessel D and Flugge UI (1983) A method for the quantitative recovery of protein in dilute solution in the presence of detergents and lipids. Anal Biochem 138: 141-143.
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Learning how to Prepare Buffers of Various pH levels and Preparation of Acetate Buffer. Introduction A buffer is a solution having the capacity to resist changes in pH levels. Mostly a buffer consists of a weak acid and a salt of strong base or a weak base and a conjugate salt of strong acid e.g. acetate buffer is the most common buffer in which equimolar mixture of acetic acid and sodium acetate solution is used. CH3COOH CH3 COO - + H+ CH3COONa CH3COO - + Na+ Buffers are divided into 2 parts
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because they contain starch. The optimum pH for pancreatic amylase is the pH of 7. In the experiment I have used buffer solutions with the pHs of 2.8‚ 4 and 6.5. I have also used iodine and starch. Normally‚ iodine is orange-yellow‚ however when you add starch to it‚ the solution will turn blue-black. Aim: The aim for this experiment is to investigate how the different buffer solutions work on the enzyme amylase. This will be investigated by using iodine and by timing the experiment using the
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discover the activity of the enzyme catecholase in different pH levels as well as its absorbance in differently concentrated solutions. A spetrophotometer was used to measure the absorbance of the enzyme catecholase in different pH solutions as well as to measure the absorbance of catecholase in solutions with different concentrations of potato juice and phosphate buffers. Absorbance of the enzyme catecholase was at an optimum level when pH was close to neutral. When pH was acidic or basic‚ the
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Joanne Kathleen Saori T. Suzuki‚ Laurent Santos‚ Val Justin D.M. Tongco* College of Science‚ University of Santo Tomas‚ Manila Philippines Abstract Salivary amylase‚ found in humans‚ is enzyme that catalyzes the hydrolysis of starch into simpler compounds. Its enzymatic activity is affected by several factors‚ such as temperature and pH. The rates of enzymatic activity of salivary amylase in different temperatures and pH were measured and resulted to be very near 50 C and 7 respectively. However
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Experiment 1: "A cis to trans conversion" Saturday‚ June 25‚ 2005 4:19 PM Notes on Theory • Alkenes o They are hydrocarbons with a C=C double bond • Double bonds are stronger and more reactive than single bonds o Hydrocarbons with double bonds used to be known as "olefins"‚ because they had an oily appearance • "Oleum" means oil • "Ficare" means make • Isomers o Geometric isomers have the same molecular formula‚ but a different geometric arrangement • They also have different physical properties
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required to work some of the problems in this chapter. 1. In which one of the following solutions will acetic acid have the greatest percent ionization? A. B. C. D. 2. Which one of the following is a buffer solution? A. B. C. D. E. 3. 0.40 M HCN and 0.10 KCN 0.20 M CH3COOH 1.0 M HNO3 and 1.0 M NaNO3 0.10 M KCN 0.50 M HCl and 0.10 NaCl Which one of the following combinations cannot function as a buffer solution? A. B. C. D. E. 4. 0.1 M CH3COOH 0.1 M CH3COOH dissolved in 1.0 M HCl 0.1 M CH3COOH
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